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肌动蛋白

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Bioenergy transfer appears in the motor-protein, actin, actomyosin, collagen-protein and so forth, so thatα-helical protein with three-chain becomes the object of study.

由于生物能量传递是在马达蛋白,肌动蛋白以及胶原蛋白等中进行的。

It plays a key role in the process of cell division, cell movement, pollen tube elongation and so on.

ADF蛋白是一种在真核生物中广泛存在的低分子量的肌动蛋白结合蛋白,在细胞分裂、细胞运动、花粉管伸长等重要的生命活动中发挥着重要的作用。

At day 4, embryoid body was found and cells were positive for actin, nestin and keratin.

培养第4天形成拟胚体,肌肉特异性肌动蛋白、巢蛋白、广谱细胞角蛋白均呈阳性表达。

Result:This example main clinical manifestation for the abdominal pain, the platoon is black then, epigastrium CT, the magnetic resonance and the gastroscopy prompt the duodenum tumor, the good duodenum tumor excision method, after the technique the pathology inspection shows the duodenum fusiform shape cell lump, the immunity group result shows tumor cell CD117, CD34, the union protein, the S-100 protein, the muscle moves the protein, finally diagnoses for the duodenum benignity the nature lump.

结果:该例主要临床表现为腹痛、排黑便,上腹部CT、磁共振和胃镜检查均提示十二指肠肿瘤,行十二指肠肿瘤切除术,术后病理检查示十二指肠梭形细胞瘤,免疫组化结果示肿瘤细胞CD117,CD34,结合蛋白,S-100蛋白,肌动蛋白,最后确诊为十二指肠良性间质瘤。

It is proposed that N RAP serves as a link between the terminal actin of the myofibril and the protein complexes at the cell membrane and thus as an organizing center in the initial phase of myofibril assembly.

小鼠伴肌动蛋白相关锚定蛋白是已知的与肌纤维生成有关的细胞骨架蛋白,但是,与此对应的人类N-RAP基因的cDNA序列及其功能一直是未知的。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞,用100 TCID50柯萨奇B3病毒(CVB3)感染心肌细胞,建立病毒性心肌炎实验模型,然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞,观察心肌细胞的形态和搏动,用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白,四唑蓝比色法测定心肌细胞活性和病毒抑制率,流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

Immunocytochemical staining was used to identify sarcomeric actin and intercalated disc-like structure when the cells were cultured for additional 1 week, 2 or 3 weeks, respectively. RESULTS: Sarcomeric actin positive cells were observed in 1 week, 2 or 3 weeks after 5-aza treatment. Some cells stained positive for connexin43 at 1 week and 2 weeks after 5-aza treatment, with brown pellet located dispersively around nucleus.

结果: 5-aza处理后1周、2周、3周均可见横纹肌肌动蛋白染色阳性细胞。5-aza处理后1周及2周连结蛋白43在部分细胞上表达:呈核周散在分布的棕黄色颗粒,5-aza处理后3周连结蛋白43 在细胞密度大的区域呈核周聚集成线形分布的棕黄色颗粒,个别细胞间可见此结构,类似正常心肌的闰盘结构。

In rat hippocampal slices, TBS induced phosphorylation of p21-activated kinase and its downstream effecter cofilin, signaling proteins that enable actin filament growth.

在鼠海马切片中,短暂快速脉冲刺激导致蛋白激酶21的磷酸化,在它的下游区产生了cofilin ,一种能使肌动蛋白丝生长的信号蛋白。

ADF/Cofilins Proteinic Family Constellation in Cytoskeletal Protein: How Does Depolymerization Occur through Combination of ADF/Cofilins with Actin?

CEPS 思博网--文章书目;-1页数:5 需求点数:20 电子全文:请登入。篇名细胞骨架蛋白中ADF/cofilin蛋白家系的作用:如何与肌动蛋白结合及发挥解聚作用?

The sensitive nymph instar and sexual differences of wing dimorphism in the brown planthopper, Nilaparvata lugens , were studied, and the developmental differentiations of both the indirect flight muscles and the wing buds in the brown planthopper were observed. Molecular biological studies were performed to amplify the indirect flight muscle specific actin gene.

本文研究了褐飞虱Nilaparvata lugens翅型分化的若虫敏感临界龄期和翅型分化在性别上的差异,观察了褐飞虱间接飞行肌和翅芽发育的分化过程,在此基础上,以扩增、克隆褐飞虱间接飞行肌特异性肌动蛋白基因为目的,做了部分分子生物学工作。

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