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肌动蛋白

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Actomyosin were purified from human heart, and further isolated into CMLC with urea degradation and ammonium sulfate precipitation, CMLCⅠ and CMLCⅡ were purified later from this CMLC by the improved PAGE method, both CMLCⅠ and CMLCⅡ were without any protein contamination.

从人心肌提取肌动球蛋白,用尿素降解法和硫酸铵沉淀法从肌动球蛋白中分离、纯化CMLC,应用改进的聚丙烯酰胺凝胶电泳法从总CMLC中分离CMLCⅠ和CMLC Ⅱ,二者均不含有任何杂蛋白。

Methods:Via a cannula iserted into adominal aorta, Gey's balanced salt solution containing 0.025% collagenase P was perfused into rat normal pancreas. After digestion with collagenase P and protease, Nycodenz density gradient centrifugation was performed to isolate PSC. PSC was identified by its morphology, cytoplasmic lipid droplets and immunocytochemical staining for desmin, GFAP and α-SMA. Results:The production,viability and purity of isolated PSC were(15.3 ± 4.6)× 103/g body weight,(95.0 ± 3.5)%, and ﹥80% respectively.

大鼠腹主动脉插管灌注后,经胶原酶消化、Nycodenz密度梯度离心,分离得到PSC;通过观察细胞形态、胞质内脂滴和免疫细胞化学方法检测结蛋白、神经胶质酸纤维蛋白(glial fibrillary acidic protein,GFAP)、α平滑肌肌动素(α-smooth muscle actin,α-SMA)的表达来鉴定PSC。

However, the role of Abl in regulating force development and actin cytoskeleton in smooth muscle has no been investigated.

而Abl在调节平滑肌肌动蛋白骨架结构中的作用及其对于平滑肌张力方面的作用则有待进一步发掘。

Various lines of evidence suggest that nuclear actin is required for efficient transcription by RNA polymerases I, II and III.

众多前人实验证据表明,细胞核内肌动蛋白是RNA聚合酶I、II和III所介导的高效率转录所必需的。

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从有历史以来,英国,在此地早期居住的是凯尔特人,已经被征服了三次。

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