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肌动蛋白

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Actin and myoglobulin are the compositions building muscles' stretching function. Microtubulin is the basic substance which provides the flagellum and cilium the function of movement.

肌动蛋白及肌球蛋白乃是构成肌肉伸缩机构的组成部分,微管蛋白是赋予鞭毛及纤毛能动的基本物质。

Endothelial cell form and contractility are regulated by cytoskeleton proteins,such as actin and myosin.

内皮细胞形态变化和收缩性的改变主要受细胞骨架蛋白如肌动蛋白和肌球蛋白的影响。

The mean proportional volumes of mucous acini were more than those of serous acini except salivary glands of rodents.Conclusion: The mean proportional volume of MeCs in salivary glands associates with animal species and secretion of salivary glands.

HHF 35是近几年开始使用的识别特异性肌动蛋白(muscle-actin-specific)的单克隆抗体,作者使用它来区别肌上皮细胞和腺泡,希望通过定量测定比较肌上皮细胞的平均体积比,以了解不同动物涎腺中肌上皮细胞的发育是否存在物种间的差异及与分泌功能的关系。

The identified proteins were divided into two following categories: one is regulatory proteins, for example, caffeic acid 3-O-methyltransferas, HSP70, glyoxalase I , actin, and so on. They are involved in protein biosynthesis and processing, cellular structural organization and stress responses. The other is function proteins, for instance, ascorbate peroxidase, cytoplasmic malate dehydrogenase, cytosolic phosphoglycerate kinase, S-adenosyl methionine synthetase, ATP synthase, and so on. They are involved in photosynthesis, photorespiration, redox homeostasis, and metabolisms.

通过分析可以将这些差异蛋白分为两大类:第一类是调节性蛋白,如咖啡酸三氧转甲基酶,热激蛋白70,醛酮变位酶I,肌动蛋白等,它们主要参与蛋白质的合成和加工、胁迫响应、细胞骨架稳定等过程;第二类是功能性蛋白,如抗坏血酸过氧化物酶,细胞质苹果酸脱氢酶,细胞质磷酸甘油酸激酶,S-腺苷蛋氨酸合成酶,ATP合酶等,它们则参与氧化还原平衡、光合作用、光呼吸和各种代谢等过程。

Dystrophin protein plays a key structural role in muscle fiber function by connecting sarcolemma with the intracellular actin filaments.

dystrophin蛋白次通过连接与细胞内肌动蛋白丝肌膜肌纤维中的功能键结构的作用。

The major muscle protei , actin and myosin, convert chemical energy to mechanical work, while protei in tendo bind muscles to bones, skin, hairy fingernails, and toenails are pertinacious protective su tance.

重要的肌肉蛋白—肌动蛋白和肌球蛋白把化学能转变成机械能;而腱中的蛋白质则是将肌肉粘连在骨骼上。皮肤、毛发、指甲是蛋白质类的保护物质。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示,甲醛刺激后19个蛋白斑点发生变化,肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点,已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ,磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6,这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示,甲醛刺激后19个蛋白斑点发生变化,肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点,已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ,磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6,这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

MSC induced by 5-aza could be identified by the positive staining for Troponin I and Connexin43. The number of cells stained positively in the 10umol L-1 and 20umol L-1 5-aza was visually greater than that of other groups , but more than 50% the cells necrosed in 20umolL -1 5-aza. Electron microscopy revealed sarcomeric organization and gap junction.

结果MSCs体外贴壁生长,呈成纤维细胞样形态,经5一aza诱导后细胞体积增大,变为球状或者长杆状,4w后荧光免疫细胞化学方法检测肌钙蛋白、肌动蛋白及Connexin43表达阳性,10umol·L一'5一aza组及20umoz·L一'5一aza组阳性表达较高,但20umol·L一'5一aza组约50%细胞死亡。

After removing DNA, histones and part of NHPs, we found that actin and tropomyosin exist in the polytene chromosomal scafiold by immunofluorescence experiments.

对去除DNA、组蛋白和大部分非组蛋白的果蝇多线染色体的免疫荧光实验表明,肌动蛋白和原肌球蛋白存在于果蝇多线染色体骨架上。

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