细胞器官

Methods The plasma levels of CGRP in 59 cases of normal pregnant women, 57 cases of PIH, and 10 cases of nonpregnant women were determined with a sensitive radioimmunoassay.

是由周围血管神经纤维末梢和甲状腺C细胞合成、分泌的，广泛分布在神经组织以及某些器官中，是体内较强的血管舒张物质，参与血压动态平衡的维持及器官血流的调节［2，3］。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The goal of animal cell technology includes accomplishments of clonal expansion of differentiated cells with useful ability, optimization of their culture conditions and pharmaceutically important proteins and the application of animal cells to gene therapy, artificial organs and functional foods.

动物细胞技术的目标有有用的能力包括使有差异的细胞的无性系的扩大的成就，他们文化状况和pharmaceutically 重要蛋白质和动物细胞在基因治疗，虚假器官和职能的食品的应用的最优化

Among its many biological activities, the major function of GM-CSF is to stimulate hematogenie stem cells prolification from silent stage to S phage, so that the stem cells can differentiate into granulocyte-macrophage cells.

有4个外显子和3个内含子，基因定位于5号染色体长臂5q21-5q32区，大多数器官均能产生GM-CSF，GM-CSF有多种生物活性，作用于多种细胞谱系，其主要作用是刺激造血祖细胞增殖即从静止期进入S期，并分化为粒细胞---单核巨噬细胞。

Objective : To measure the differences of total T cell percentages of peripheral blood, peripheral immune organs and central immune organs of maters of interspecific, intraspecific embryo implantation and pseudopregnant mice at corresponding time, and to analyze the effects of these differences on interspecific embryo implantation.

目的：研究种间胚胎植入期母体外周血、外周免疫器官、中枢免疫器官中总T细胞的百分比变化，并探讨这种变化对种间胚胎植入的影响。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中，雏鹅临床表现为高发病率和高死亡率，食欲和饮水减少，拉白色、黄绿色稀粪，伴有神经症状；常规组织学观察，脾脏和胰腺肿大，有白色坏死斑点，肠道出血和坏死，肝脏肿大变性，肾脏肿大变性有尿酸盐沉着等；用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究，结果表明：胰腺中发现病毒包涵体，肠道中大量纤维素以及胶原纤维增生，肾脏、肺脏大量含铁血黄素沉着，免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少；电镜下多种细胞呈现线粒体水肿，多种实质细胞的染色质固缩，染色质边集，特别是细胞内容物如脂肪滴增多的病理变化特征。

To attain more comprehensive understanding of flounder immune system, it is necessary to characterise and localise lymphoid cells within the different lymphoid organs. Use polyclonal antibody or monoclonal antibody as a tool will benefit this kind of study.

制备针对免疫球蛋白的多克隆或单克隆抗体，作为研究工具，对免疫器官中产生抗体的组织和细胞进行定位，可以了解免疫器官在防御疾病过程中是如何发挥作用的，以便对应用疫苗奠定理论基础。

Each male ratwas paired with 2 female rats in proestrus when administration terminated. Femalerats were examined the next morning for the presence of sperm in vaginal smears andunderwent a cesarean section on day 13 of gestation. Then the reproductive indiceswere calculated as follows: copulation index, pregnancy index, and fertility index,which can evaluate male fertility directly. The sperm motility and morphology wereevaluated by computer-assisted sperm analysis, moreover, the caudaepididymal sperm ultramicrostructure were analyzed by transmission electronmicroscope, which can evaluate the influence on sperm maturation bydutasteride ; sperm survival rate was assessed by SYBR-14 and propidium iodidefluorescent staining; serumal dihydrotestosterone and testosterone of ratswere detected by enzyme-labeled immunoassay, which can evaluate the effecton androgenic levels; the weights of testes and epididymides, were determined byprecise electronic balance; histological examination of above tissues were evaluatedby HE staining and TEM, which can evaluate treatment on histology of reproductiveorgans by dutasteride.

给药结束后雄鼠与处于动情前期的雌鼠按2：1合笼，计算雌鼠的交配指数、受孕指数和生育指数，直接评价雄鼠生育力；采用计算机辅助精子分析系统分析大鼠附睾精子活力和形态，在此基础上合并使用透射电镜技术对各组附睾尾部精子进行透射电镜分析，评价度他雄胺对大鼠精子成熟的影响；采用SYBR-14和propidium iodide双重荧光染色计算精子存活率；采用Elisa法测定大鼠睾酮(testosterone， T)和双氢睾酮(dihydrotestosterone， DHT)血清浓度，评价度他雄胺对雄鼠雄激素水平的影响；采用精密电子天平对各组睾丸、附睾、前列腺和精囊进行称重，评价度他雄胺对大鼠生殖器官重量的影响；采用HE染色法对各组睾丸和附睾进行组织学分析，同时采用透射电镜技术对附睾上皮细胞超微结构进行分析，评价度他雄胺对大鼠主要生殖器官组织学的影响。

It is zeatin that mainly determines root activity and sustainleaf physiological function. However, not every kind of cytokinins hasthe capacity to promote or keep physiological function.

决定根系活力和维持叶片生理功能的细胞分裂素是玉米素，但是，不是每一种细胞分裂素都有促进或保持生理活性的作用，部分种类的细胞分裂素很可能与器官的衰老有关。

It is zeatin that mainly determines root activity and sustainleaf physiological function.However,not every kind of cytokinins hasthe capacity to promote or keep physiological function.Some kinds ofcytokinins are related to senescence.

决定根系活力和维持叶片生理功能的细胞分裂素是玉米素，但是，不是每一种细胞分裂素都有促进或保持生理活性的作用，部分种类的细胞分裂素很可能与器官的衰老有关。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。