测定...的时间

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

This paper illustrates the potential of cosmogenic neon exposure age in studying the eruption., surface morphology, and erosion history of young volcanic rocks, which are difficult to date using other conventional methods,such as K-Ar or 40Ar/39Ar dating.

本论文阐述了宇宙中氖的暴露时间在火山爆发，表面形态和新形成的火山岩的侵蚀过程研究中的潜力。这些研究用常规的方法，如钾-氩或40氩/39氩测定年代较难进行。

Some important control parameters,including the concentration of samples,the pumping rate,the ultrasonic intensity and dispersion time,and the selection of the deflocculant,are optimized for the establishment of a standard operation process for soft clay.

采用马尔文公司生产的Mastersizer Micro(MAF5000)激光衍射粒度仪测定江苏连云港地区软土的粒度分布，对样品质量浓度、泵速、超声波强度与时间、分散剂选取等控制参数进行了优化设计，确定合适的控制参数，便于对相同类型的软土测量确定标准操作规程。

To evaluate the potential of several biodiesels, which had different content of fatty acid methyl ester, as substitute solvent for quizalofop-p-ethyl emulsifiable concentrate, the type and content of fatty acid methyl esters in tested biodiesels were analyzed by gas chromatography, and 5% quizalofop-p-ethyl ECs were prepared with different biodiesels, respectively. The control test against gramineous weeds by the 5% quizalofop-p-ethyl ECs were carried out in greenhouse.

为评价不同来源生物柴油作为溶剂配制精喹禾灵乳油的效果，采用气相色谱法测定了几种生物柴油中脂肪酸甲酯的种类及含量，利用相应制备的5%精喹禾灵乳油进行了防治禾本科杂草的盆栽试验，同时比较了不同生物柴油对药剂稀释液表面张力、干燥时间和在叶片表面沉积量等药液物理性状的影响。

The article reviews the advanced research on screening sheepbreeds with high resistance against fasciolosis. Screening sheep breeds with high resistance against fasciolosis has been tested by measuring parameter of plasma such as packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin concen tration, glutamate dehydrogenase, gamma glutamyl transferase and aspartate trans ferase and percent take of metacercariae. High resistance of Indonesian thin tail sheep against Fasciola gigantica has been confirmed. The mechanism of high res istance and the time of killing Fasciola gigantica has also been described.

本文综述了抗片形吸虫病绵羊品种的研究概况，通过红细胞压积、红细胞平均体积、红细胞平均血红蛋白浓度、血清谷氨酸脱氢酶、γ谷氨酰胺转移酶、天冬氨酸转氨酶等血液学参数，囊蚴感染成活率的测定等方法来筛选抗片形吸虫病的绵羊品种，研究证明印度尼西亚细尾绵羊对大片吸虫感染具有较高的抵抗力，并概述了其抗虫感染的机制及杀虫作用时间。

Results. Semiquantitative assessment of histologic changes indicated that 4-week-old rabbit was equivalent to fetus to 2-year-old human and 160-week-old rabbit was equivalent to 11- to 30-year-old human, particularly 11- to 16-year-old, which corresponds to transition period from notochordal to fibrocartilaginous NP.

结果：组织学改变上的半定量测定显示，4个月的兔子相当于2岁的孩子，160个月大的兔子相当于11到30岁大的成人，尤其相当于11到16个月大，相对地，这段时间正好是脊索向纤维软骨NP转换的时期。

Results. Semiquantitatie assessment of histologic changes indicated that 4-week-old rabbit was equialent to fetus to 2-year-old human and 160-week-old rabbit was equialent to 11- to 30-year-old human, particularly 11- to 16-year-old, which corresponds to transition period from notochordal to fibrocartilaginous NP.

结果：组织学改变上的半定量测定显示，4个月的兔子相当于2岁的孩子，160个月大的兔子相当于11到30岁大的**，尤其相当于11到16个月大，相对地，这段时间正好是脊索向纤维软骨NP转换的时期。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Four kinds of red oxisols (fine, mixed, acid, hyperthermic paleusult), with diverse capacity of adsorption, were mixed with 50ppm and 100ppm Cu(superscript +2)/Cd(superscript +2) and measured the ion concentrations of Cu(superscript +2)/Cd(superscript +2) under inequilibrium state at different time during the adsorption process.

本研究使用吸附能力不同的四个大度山红壤分别与50ppm和100ppm的铜离子或镉离子溶液混合，在吸附反应的过程中，测定未平衡状态不同时间的金属离子浓度，可以得到动态吸附现象的数据。

Membrane permeability, malondialdehyde contents and activities of antioxidant enzymes in Kochia prostrata seedlings were studied under different concentrations of salt stress. The result showed that the membrane permeability and MDA contents increased with the increasing of salt concentrations.

通过不同浓度的盐胁迫对木地肤Kochia prostrata幼苗细胞膜透性、丙二醛和3种保护酶活性的测定，获得以下结果：随着盐浓度的增加和胁迫时间的延长，幼苗叶片的细胞膜透性呈递增趋势，且浓度≥150mmol/L时，细胞膜透性与对照有极显著差异(P.01)。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。