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The infrared spectrum and main anionic properties were tested. Levelling ability in wool dyeing indicated that LJC-1 can solve the problem of unlevel dyeing of wool effectively.

讨论了其对弱酸性色性能的影响,其在羊毛色中的匀性能表明:匀剂LJC-1能有效地解决羊毛色中色不匀问题,是一支性能优良的匀剂。

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只,随机分为电穿孔组和非电穿孔组,每组9只,制作右下肢趾长伸肌失神经支配模型;EP组为将质粒pEGFP-N1溶液50μl(0.8μg/μl)注射入右趾长伸肌后,立即于两侧腱腹交接处给予电穿孔,电穿孔参数为:电场强度为200V/Cm,脉冲100μs,频率1Hz,施加10次脉冲;NEP组仅质粒pEGFP-N1溶液注射;转后1、2、3周,荧光显微镜下观察趾长伸肌中GFP的表达情况,转后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况,检测和优化体内转效率。2、健康雌性SD大鼠78只,随机分为失神经对照组、RC2基因治疗组(RC2组),MAFbx基因治疗组,每组24只,制作右下肢趾长伸肌失神经支配模型,余6只为正常组;分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌,之后给予电穿孔,方法同上;治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化,治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积,肌细胞超微结构变化以及肌纤维中蛋白含量变化。

METHODS: The MGC-803/ E2F-1 gastric cancer cells which were stably transfected with E2F-1, and containing unpossessed carriers, and the cells without transfection were performed MTT test to draw growing curve, and to compute cell proliferation rate. Cell cycles were examined by flow cytometry.

对稳定转E2F-1的胃癌MGC-803/E2F-1细胞、转空载体的MGC-803/EV及未转的MGC-803细胞进行MTT法检测,绘制生长曲线和计算细胞增殖率;流式细胞仪检测各组细胞周期分布。

In this study, we developed the ammonia sensing material by using MCM-41 impregnated with bromocresol green. MCM-41 is one kind of mesoporous molecular sieves. It can be applied to many fields due to well-defined pore shapes, narrow distribution of pore sizes, large pore volumes, high surface area, excellent thermal, hydrothermal, chemical and mechanical stability. And the bromocresol green dye is commonly used as a pH indicator with colorimetric from pH 3.8 to pH 5.4.

本研究之目的是结合变色剂及中孔洞分子筛MCM-41进行氨气吸附,研究中所选择之变色剂溴甲酚绿是一种偏酸性之大分子有机剂,其变色范围在pH 3.8到pH 5.4;而MCM-41由於其孔洞大小分布范围狭窄、孔洞体积大、高比表面积、具有良好的吸附容积、表面反应性强及易於改质等优点,故期望利用此复合材料,经气体吸附达到氨的移除并同时在吸附饱和前能产生显色效果。

Cotton and silk fabrics were dyed with natural carminic acid after the Iron ion pre-mordant treatment. The K/S values of the fabrics were characterized. The single factor process experiments were carried out for mordant concentration, temperature and time. The optimal process parameters were mordant concentration cotton: 1×10^(-3mol/L and 10 min at 90℃; silk: 1×10^(-3)mol/L and 10 min at 40℃.

将铁离子前媒处理后的棉、丝织物采用天然料胭脂虫酸进行色,以色织物的K/S值进行表征,对媒剂浓度、温度、时间3个因素进行了单因素工艺实验,得到了铁离子前媒棉、丝织物的最佳工艺参数(棉:1×10^(-3)mol/L, 90℃,10分钟;丝:1×10^(-3)mol/L, 40℃,10分钟)。

As long as you control the boiling yeum time dyeable good color, which can help to dyes, easily manipulated to 0.5% to relatalion 1.5%.

只给把持好沸工夫不离可好颜色,便于操纵,缓剂用量为0.5%~1.5%。

The technique and process for producing differential dyeable slub PET filament yarn are introduced, the differential dyeing principle is analysed, and the influences of the spinning lateral blow.

介绍了异型涤纶竹节丝的生产技术与工艺流程,分析了异原理,讨论了纺丝侧吹风、后拉伸倍数、拉伸罗拉绕丝圈数、拉伸温度、定型温度等工艺参数对成品品质与异效果的影响。

Argazol EC reactive dyes have excellent compatibility. It has excellent stability, especially suitable for sensitive shades such as olive, coffee, gray and so on . Argazol EC reactive dyes are highly stable to liquor ratio, salt , alkali, fixation time, so dyehouse can get excellent reproducibility.

雅格素 EC型活性料具有优异的配伍性,卓越的稳定效果,适合橄榄绿、咖啡、灰色等较难的颜色,对浴比、温度、盐、碱及固色时间的敏感性小,有优异的色重现性,配合合理的工艺,能充分满足工厂高的&一次成率&的要求,具备优良的各项牢度。

Methods: Mice were inoculated with tumor cells. Inhibitory effect of transfection with pCH510 on murine tumor origined from different inoculative dose and inhibiting effect of immediate transfection pCH510 after chemotherapy on tumor were observed, respectively. By cell culture technique, the influence of chemotherapeutic drug to activation of marcrophages and lymphocytes after i.p. injection of drug was observed. By cell counting method, the kinetics of the change of number of peripheral blood immunocytes induced by administration of chemotherapeutic drug was observed. The inhibitory effect of transfection with pCH510 five days after chemotherapy on murine tumor was observed.

采用瘤细胞接种建立小鼠肿瘤模型:通过基因转,观察pCH510对不同接种量所形成的小鼠肿瘤的抑制作用以及小鼠肿瘤化疗后立即进行pCH510转的抑瘤效果;采用细胞培养技术,观察小鼠体内注射化疗药物后,其对小鼠腹腔巨噬细胞和脾淋巴细胞激活功能的影响;采用细胞计数的方法,观察化疗药物所致小鼠腹腔巨噬细胞和外周血免疫细胞数量变化的动力学;另外小鼠肿瘤化疗后第5天进行pCH510转,观察抑瘤效果。

Adult rats are divided into control group, intrapericardial injection group and negative group, sublingual vein injection group and negative group. 6ds after transfection, issues of heart, liver, lung and kidney are stained with X-gal to observe transfection to myocardium and non-target transfection. 3. CaN Aβ gene silencing in vivo. Adult rats are divided into Control group, hypertrophy model group, intrapericardial injection group and negative group, sublingual vein injection group and negative group.

二整体动物水平上探讨心肌有效转途径:以PLacZ为报告基因,将成年大鼠分为空白对照纽(生理盐水心包腔内注射后超声导入);转质粒心包腔内注射组及其阴性对照组:质粒+微泡+酶的混合物心包腔内注射,超声导入;转质粒舌下静脉注射组及其阴性对照组:质粒+微泡混合液经舌下静脉注射,超声导入。

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