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PAP-C23, amplified from mature pokeweed antiviral protein gene by PCR, was cloned into the expression vector PET101, and then the recombinant plasmid was transformed into Escherichia coli BL21(DE3). The recombinant mutant PAP was expressed high-levelly induced with IPTG.

用KCL溶液染SDS-PAGE胶，切下表达的目的蛋白，冰浴研磨后用磷酸缓冲液溶解，再加入等体积的福氏不完全佐剂，乳化完全后，免疫家兔，制备抗PAP蛋白的多克隆抗体，间接ELISA法测定所制备的抗血清的效价为1：1000。

In the normal rats (n=5、6), CGRP-LI were mainly present in small and some medium-sized neurons and the primary afferent fibers and ter- minals in laminae Ⅰ,Ⅱ,Ⅴ,Ⅹ and Lissauer's tract of the spinal cord; GAL- and SP-LI were mainly present in small neurons and the superficial layers of the dorsal horn. After the adjuvant injection both CGRP-and SP-LI were in- creased corresponding to the development of arthritis. On day 2, CGRP-and SP-LI were moderately increased in laminae Ⅰ,Ⅱ and the posterior commis- sure of the spinal cord and small neurons in DRG, while SP was obviously in- creased in the superficial layers and DRG. On day 14, CGRP-LI were marked- ly enhanced in the above regions with the development of polyarthritis. Some- times many strongly stained long fibers could be seen in the posteriot commis- sure.

在DRG中，CGRP主要分布于小和中等神经元之中，SP和GAL主要分布于小神经元之中；2、注入弗氏完全佐剂（含冻干结核菌0.5mg）后第2天动物（各5~6例）产生具有明显红、肿、热、痛和痛敏的急性关节炎，免疫组化实验观察到注射侧脊髓背角和DRG中CGRP-和SP-LI明显增加；在佐剂后第14天，动物患肢肿胀、痛敏和自发痛均进一步加重，部分动物健肢也出现红肿，产生多发性关节炎时，脊髓背角浅层和DRG中CGRP-和SP-LI增加更为明显，并且在灰质后联合中可见许多深染的CGRP-LI长纤维；在佐剂后第21和28天，随着佐剂性关节炎的逐渐恢复，脊髓背角和DRG中CGRP-和SP-LI也接近正常。

By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段，用双脱氧链终止银染测序法测定了该片段的全序列，发现它含有一个完整。。。

B: i'd love to have my hair pomade .

我想把头发染一下，然后再稍微烫一下。。。

"Jeez," Jack muttered, brushing back a recently styled silver-tinted pompadour,"et too?"

"上帝"杰克喃喃自语，将自己刚刚染成时尚银色的头发捋到后面，"你也这样？"

L/S yarn dyed poplin stripe spread collar 1 open PKT tail bottom .

L/S纱染府绸条纹扩展到衣领1开口处，后面又是什么意思啊

Droperidol （ potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道，可能是 QT 间期延长的机制。

One was the successful introduction of the power loom into American manufacture; the other was the organization of production so that all four states of the manufacture of cotton cloth could occur within one establishment.

一是动力织机被成功引进美国制造业；二是生产的有序化使棉布制造的四个过程，即纺、织、染、剪，可以在同一工厂完成。

They themselves did not go into the Praetorium, so that they might not become unclean, but might take the Passover.

18：28 众人将耶稣、从该亚法那里往衙门内解去，那时天还早，他们自己却不进衙门、恐怕染了污秽、不能吃逾越节的筵席。

Then they led Jesus from Caiaphas into the Praetorium, and it was early; and they themselves did not enter into the Praetorium so that they would not be defiled, but might eat the Passover.

约18：28 众人将耶稣、从该亚法那里往衙门内解去。那时天还早。他们自己却不进衙门、恐怕染了污秽、不能吃逾越节的筵席。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。