染

Based on introducing the control principle of the jigger system, the analysis of control requirement for the system was achieved, and the control scheme was proposed. The construction of hardware platform based on programmable logic controller was built. The control strategies about the velocity-tension transmission system and the temperature of dyeing liquor were presented.

在介绍卷染机控制原理的基础上，对系统的控制要求进行了分析，并确定了控制方案，搭建了基于可编程逻辑控制器的卷染机硬件控制平台，提出了卷染生产过程中速度张力传动控制策略和染液温度控制策略。

The results showed that the acid releaser HTA was able to lower the pH values gradually and to increase the levelness without changing the dye fastness.

探讨了释酸剂用量、加入方式以及起始pH值等对锦纶弱酸性染料染色的上染速率和上染率的影响，结果表明：释酸剂HTA能逐渐降低染液的pH值，在不改变染色牢度的前提下，可提高匀染性

The level of VEGF mRNA was elevated after 6h when 8Gy irradiation was processed and maintained to 72h. Sense and anti-sense expression vector were transfected into vascular endothelial cells and stable cell lines were established. Cell line transfected sense expression vector of VEGF_(165) gene showed reduced inhibition of prolifetation, MNF, MNCF and apoptosis compared to that of control vector, while opposite results were obtained in anti-sense transfected cell line.

转染正义表达载体的ECV-304稳定细胞系经过4Gy γ射线照射后，与转染空载体的细胞相比，细胞增殖抑制率减弱，MNF、MNCF以及凋亡减少；而转染反义表达载体的细胞系与转染空载体的细胞相比，细胞增殖抑制率增强，MNF、MNCF以及凋亡增加。

To observ the toxicity of infected mice and establish mice reproductive toxicity model;②To observe the infected BALB/c female mice ovulation quantums and ovum chromosome;③To observe the oestrogenic hormone( FSH, LH, E2 )level of infected BALB/c female mice;④To observe zygote developmental condition before nidation;⑤To observe embryonic development condition of infected mid-gravidity mice to research the reproductive toxicity and development toxicity of toxoplasma gondii to the BALB/c female mice.

目的： 本实验采用弓形虫感染BALB/c雌鼠，①观察染虫雌鼠的毒性反应，建立生殖毒性的小鼠模型；②染虫雌鼠排卵情况及卵母细胞染色体的变化；③染虫雌鼠性激素(FSH、LH、E2)水平的变化；④着床前受精卵的发育情况；⑤孕中期鼠染虫后胚胎的发育情况，探讨弓形虫对雌鼠生殖功能及胚胎发育的毒性作用。

Methods In this study,a plasmid vector,rAd5,and rAAV2 were used to transfect rat cochlear marginal cells of stria vascularis by injection into the perilymph through the round window membrane,and the transfection efficiency, target tissue accessibility, cell/ tissue toxicity, time course of expression, and effect on hearing were evaluated in vivo and in vitro.

结果三种载体对原代培养的大鼠耳边缘细胞的转染研究发现，腺病毒的转染效率最高，而腺相关病毒转染对细胞活性影响最小。rAAV2和rAd5对大鼠耳蜗组织的感染实验发现，腺病毒和腺相关病毒携带的增强型绿色荧光蛋白可在多种耳蜗组织中及转染对侧耳蜗组织表达，且并不引起明显的耳蜗组织细胞凋亡。rAAV2携带的EGFP基因在第90天时仍可检测到大量表达。rAd5携带的EGFP基因在第30天时表达明显减弱。

Adenovirus 5第二军医大学博士学位论文英文摘要胸心外科专业(WAd5) and PDC-MLC-VEGF were transferred to the adenoviral packaging cell HEK 293cell by lipofectamine 2000 mediated gene transfer method to pack the virus; The desired Advectors were purified by density gradient ultracentrifuge and titrated in 293 cells afteridentified.

Ad.mlc-hVEGF165 体外转染心肌细胞的实验研究分离培养乳鼠心肌细胞，在同一 MOI 值下，Ad.mlc-hVEGF165 和 Ad.hVEGF165分别转染心肌细胞，以 RT-PCR 法和 Western 印迹分析法分别检测转染后心肌细胞内VEGF mRNA 的转录及 VEGF 蛋白的表达，以 ELISA 法检测转染后心肌细胞分泌的VEGF 蛋白质，MTT 等方法观察其对促内皮细胞增殖作用。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

The "color dreams" dyeing of Ningxia beach Woolfell was carried out using the environment-friendly discharge agent from German Basifu compary, combined with intermittment discharge technology by the means of water bath dyeing, spray-painting dyeing and knot printing dyeing, fuming and braisign, and so on.

采用德国巴斯夫公司环保型拔染剂，结合水浴染、喷染、印染、扎染、熏蒸等方式的间歇式拔染技术，实现宁夏滩羊毛皮"梦幻色"染色。

Shandong Shengli Bioengineering Co., Ltd., Jining 272037, Shandong, China Abstract: The cause of infection in the process of avermectin production was studied from infected time, scope and bacterial type. The test method to check the infection caused by other bacteria was also raised. The ways to prevent infection was ascertained from preparation of microbial strain, air purification, medium disinfection, ductwork deployment and systematic dead angle cleaning. Meanwhile, adjustment measure was summarized in different infected period such as microbial strain, seed pot and yeastiness.

山东胜利生物工程有限公司，山东济宁 272073 摘要：从染菌的时间、类型、范围三方面探讨了阿维菌素发酵染菌的原因，提出了发酵过程中杂菌的检查方法，分别从菌种制备、空气净化、培养基灭菌、管路配置、系统死角清理等方面明确了防止发酵染菌的措施，得出了斜面菌种、种子罐、发酵罐不同时期染菌后的补救措施。

Starting from the solubility of disperse dyes, the solubility, up-taking rate, migrating rate of dis_perse Yellow M-4G, Red 3B and Blue 2BLN at different concentration of Leveling Agent GS were tested by poly_mer thin-film bag method, thus demonstrating the hyperchromic effect and achromic effect of leveling agent during dyeing with disperse dyes.

从分散染料溶解度的角度出发，采用聚合物薄膜袋法测定了分散黄M－ 4G、分散红3B、分散蓝2BLN 三种分散染料在匀染剂GS 不同浓度时的溶解度以及上染率、移染率，从而展示了匀染剂在分散染料染色中的&增色效应&和&消色效应&，并从染色理论上推论了产生这两种效应的原理。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。