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Finally, we also examined the diferentiation efect of Ad-GFP on myoblasts.3.Study of the transfection of Ad-GFP to myoblasts which Transplanted in the intrarectal of the immune defect rat.The immune defect rats Were randomized in to 2 groups( 10 in each group):The experimental group transplanted with myoblasts transfected by Ad-GFP; the control group injected PBS.

并观察转染了Ad-GFP后是否会对成肌细胞的分化融合有影响。3、转染Ad-GFP的成肌细胞在免疫缺陷小鼠直肠内移植的实验研究将20只免疫缺陷小鼠随机分成2组(每组10只)，移植组肛管直肠粘膜下层注射转染Ad-GFP的成人成肌细胞，对照组注射PBS。

Swr1400 Series is the kind of dye jigger with the biggest cloth roll and the widest cloth.It is self-designed and developed by our plant based on absorption of advanced technology of various dye jiggers from Japan,Denmark,italy,etc and in the light of specific conditions.It is the essential dyeing equipment in the dyeing and puinting trade.

SWR1400系列常温常压巨型卷染机是目前国内卷布直径和布幅最大的卷染机，本机在吸收了日本、丹麦及意大利等国各种卷染机的先进技术，并结合广大用户实际情况，由我厂自行设计开发的，是印染行业不可缺少的染色设备之一。

There is a long standing debate about the magma origin of Lutao. Two different models of crust contamination and source contamination are proposed. Combining helium results with strontium ratios of Lutao lavas, it shows that Lutao arc magma has experienced approximately 5% of crust contamination.

绿岛岩浆成因长久以来存在有地壳混染或是源区混染的争论，本研究利用绿岛斜辉石及堇青石巨晶的3He/4He比值及其母岩浆的87Sr/86Sr比值，得到了绿岛岩浆经历过5%左右地壳混染的结果。

By using liposome-mediated gene transfection technology, plasmid pCMVSV40T/PUR containing SV40Tag was transfected in primary embryonic precartilaginous stem cells, while non-transfected cells served as negative controls.

利用脂质体介导基因转染技术将含有SV40Tag的质粒pCMVSV40T/PUR转染原代胚胎前软骨干细胞，以未转染细胞作阴性对照。

All the transfected and un-transfected rMSCs were attached to Allo-DBMs. These new biomaterials were implanted in muscle bags and segmental radius defects of the New Zealand white rabbits, and some controlled material groups were established for comparison. All the biomaterials and the controlled materials were assessed by gross observation, radiographical and histological methods.

取兔自体骨髓基质细胞培养扩增，用脂质体介导的方法转染人骨发生蛋白2基因，将转染和未转染人骨发生蛋白2基因的自体骨髓基质细胞附和于异体兔脱钙骨基质形成新的生物植骨材料，连同空白及单纯脱钙骨基质对照组植入兔前肢肌袋和桡骨缺损。2、4、6、8周分别取材进行大体，放射线和病理观察。

The late phase expression cassette suited for adenovirus vectors was also constructed. The HBV S gene, HBV preS2+S gene and the late phase expression cassette +HBV S genes were respectively cloned into the Ad5 vector downstream of the E3 promoter. Semiconfluent monolayers of 293 cells were cotransfected with the above constructed plasmids and Ad5 DNA EcoRI A fragment. The progeny adenoviruses named rAd5S, rAd5MS, rAd5S2S were harvested.

通过共转染优化实验，建立了脂质体转染腺病毒的有效方法，并构建了适合腺病毒载体的晚期表达盒，进一步将乙型肝炎表面抗原基因、HBV preS2+S、腺病毒晚期表达盒+HBV S基因插入E3区，与EcoRI消化的Ad5 DNA A片段共转染细胞，并获得重组腺病毒，相应命名为：rAd5S、rAd5MS、rAd5S2S。

In the process of dyeing polyester fabrics, this aggregation causes dyeing polyester fabrics, this aggregation causes dyed polyester fabrics to appear unleveled or specked.

在染多元酯织物的程序，这一个集合引起染多元酯织物，因素染了多元酯织物出现的这一个集合不消除或者斑点了。

Of CBMC can be purified by Mini-MACS as CD34〓 stem cells. B. The number of CD34〓 stem cells can expand to 40.24±9. 86 fold after 14 days. C. No matter in the expression of CD1a, CD80, CD86, and HLA-DR, or in the function of stimulating xenogenous lymphocyte proliferation, there was no difference between CD34+ stem cell DCs or monocyte DCs. D. The percentage of CD3〓CD56〓 cells is the same in CIK cells co-culture with DCs transfected with SKOV3 RNA, CIK cells co-culture with DCs, and CIK cells. E. The expansion rate of CIK cells can be accelerated by co-culturing with loaded or unloaded DCs. However, the expansion rate between loaded or unloaded group is the same. F. The strongest cytotoxicity against SKOV3 cell line was achieved by CIK cells co-cultured with DCs loaded with SKOV3 RNA.

结果：1、Mini-MACS分选系统可自CBMC中提取0.78±0.31%的CD34〓细胞；2、体外培养14天后可获得原始CD34〓细胞量40.24±9.86倍的细胞；3、不论在CD1a、CD80、CD86及HLA-DR的表达上，或是刺激异体淋巴细胞增生的功能上，脐带血CD34〓细胞与单核细胞来源的DC都没有差别；4、CIK细胞中CD3〓CD56〓双阳性的表达率在与RNA转染DC共培养的CIK细胞组、与DC共培养的CIK细胞组及单纯CIK细胞组3组间比较无差异；5、脐带血CIK细胞增殖显著，培养14天时可扩增18.18±5.59倍，培养21天时可扩增35.02±6.30倍；5、与未转染或转染DC共培养的CIK细胞在培养第14天后增殖速率大于单纯CIK细胞。

Results The cells transfected with E1AF is remarkably more invasive than those of control group. The image analysis and PCNA expression of the tumor cells demonstrate that transfected cells are m ore actively proliferative than the untransfected cells.

结果 转染后细胞的侵袭能力较转染前明显增强，肿瘤细胞图像分析和PCNA的表达，均显示转染后的肿瘤细胞增殖明显活跃。

Results UCMSCs could be effectively infected by adenovirus containing GFP gene in vitro, the transfection efficiency has the dose-effect relationship with multiplicities of infection.

结果 腺病毒介导的GFP基因对于UCMSCs具有较高的转染效率，转染效率与MOI值具有量效关系，当MOI为100倍时，转染效率达95%。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。