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Recombinant expression vector k14/c-myc-paav was constructed bearing the mouse c-Myc cDNA derived by the skin specific human keratin 14 promoter, then the linear vector was microinjected into the two pronucleus of the C57BL/6 fertilized eggs to produce transgenic mice.256 microinjected eggs were transferred, 6 of the foster mothers were pregnant and 44 adult mice were produced, two of them were positive transgenic mice confirmed by PCR and Southern blot analysis, transgenic rate was about 4%.

以CMV-PAAV载体为基础载体,以皮肤特异人K14(人角蛋白14)基因启动子替换原质粒中的CMV启动子后,在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列,构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术,将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中,然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中,共移植了256枚受精卵,6只受体怀孕,最终获得44只成年小鼠。

Experimental results show the behavior clips segmented by this algorithm are prototypical and meaningful.

能否准确地将一个有意义的行为序列从整个视频序列中分割出来,直

In this paper, we use computer to generate pseudo random number and low-discrepancy sequences.

在本文中,我们利用计算机分别产生了伪随机数序列和低差异数序列

Pseudorabies virus ; Glycoprotein G ; Nucleotide sequence ; Amino acid sequence ; Gene expressing

伪狂犬病病毒;糖蛋白G ;核苷酸序列;氨基酸序列;基因表达

By producing pseudorandom number series, we rectify the power spectrums of normal random inputs in the same shape as power spectrums of simulating signals. Through Laplace transformation, we can acquire the frequency response functions of rectifier. Two simulating examples were presented. The power spectrum curves based on one peak function model were almost coincided with the power spectrum curves of simulating loads shown in the figures. Simulating results of input loads had good accuracy. Creating a simulating method and program of input load in this paper was effective.

本文利用随机过程仿真的一般理论在已知随机载荷的分布特性、均值、方差和功率谱函数以后,实现随机载荷的计算机仿真,即利用仿真技术得到符合已知载荷的分布特性和功率谱特性的随机载荷的时间序列。1 产生伪随机数序列及分布整形采用乘同余法产生伪随机数的递推公式为[2 ] :yi+1 =λyi ( 1 )式中M =2

The standard Bruker pule sequence NOESYGPPR1D , which is a presaturation experiment for water suppression incorporating the first increment of the NOESY pulse sequence and a spoil gradient .

期待有人能为我解答这个压水峰的脉冲序列做实验时用到这个脉冲序列压制,但不知道原理,文献中提到

A putative signal peptide of 28 amino acids was predicted in the N-terminal region of all three proteins.

在这三个基因推测的氨基酸序列中,N端都存在一个含有28个氨基酸残基的推测信号肽序列

The paper introduces a method of query, i.e., window query about time series, which is the integrative technique of forward query and inverse query.

本文介绍了一种关于时间序列的窗口查询,它是一种关于时间序列的前向查询与反向查询的综合查询技术。

Firstly, image is decomposed into two serials, which are predicted and updated by two-order Neville filter on rectangle and quincunx grid by turns.

首先将图像分解为两个序列,用2阶Neville滤波器构造预测和更新算子,对两个序列以矩形栅格和梅花形栅格的格式进行交替预测和更新;再以各个波段的方差作为融合的特征,进行特征级第二代小波加权融合,最后对图像进行第二代小波重构。

We consider Theorem 1 to be highly significant and apply it to obtaining the rank of a certain augmentation quotient group by proposing Theorem 3 and giving its complete proof.

应用具有Np-序列有限p-群的特殊性质和重量函数,基本序列等概念以及已有的一些结果,分别研究了类为1的pk(k 2)阶A bel基本p-群和类为2的p4阶基本p-群之增广商群Qn的结构,得到了当n足够大时Qn作为A bel基本p-群的秩。

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推荐网络例句

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。