序列

The aim of this project was to obtain the flanking sequence of phycoerythrin gene operon,to characterize the structure and gene expression regulation function of the sequence.

本课题拟得到藻红蛋白亚基基因操纵子的旁侧序列，研究该序列的结构及基因表达调控功能。

Cloning and sequencing of the genes coding a and 3 subunit of phycoerythrin of a red alga-Corallina officinalis are reported firstly(GenBankaccession number: AF510986). The sequence contain 1,140 nucleotide, including 10 nucleotide that locate in the upstream of the initiation codon ofP subunit, 534 coding for P subunit(coding 177 amino acid), 101 nucleotide for gene spacer and 495 nucleotide that draw near the 5 terminal of a subunit(coding 164 amino acid).

首次测定了珊瑚藻藻红蛋白α和β亚基基因的全序列(GenBank登录号：AF510986)，测定的基因序列长为1，140个碱基，包括β亚基起始密码子上游的10个碱基，6亚基编码区的534个碱基(编码177个氨基酸)，101个碱基的间隔区以及α亚基编码区近5'端的495个碱基(编码164个氨基酸)。

Here we report a total of 39.9 Mb of expressed sequence tags from 29 animals belonging to 21 phyla, including 11 phyla previously lacking genomic or expressed-sequence-tag data.

在这里，我们报告从29属21门动物，其中包括11门动物以前所缺乏的基因或表达序列标记的数据动物的39.9表达序列标签MB的总数。

ITS1 and ITS2 length of Phyllodium pulchellum is 225 bp and 209 bp.

排钱树的ITS1序列长度为225 bp， ITS2序列长度为209 bp，应可为区别。

It presents a novel hypothesis that the limit angle of the opposite phyllotaxis in plants is a πω2 by the theory of analogy at first time.

采用差分方程理论和互生植物叶序分数序列的极限ω2 导出植物对生叶序序列和叶序分数。

The species observed can be categorized into 5 kinds of Fibonacci sequence groups based on the sequence of phyllotaxy and golden divergence angle. At the same time, by comparing the existing documentation, we explore the difference and relation among different taxa, in addition, the difference between environments in order to explicate the factors influencing the sequence of phyllotaxy.

藉由叶序之序列及黄金分离角，可将观察之物种，分为5种费氏序列类群，同时比对既有的文献纪录，探讨不同分类群间的差异，以及分类群间的关系，并分析环境之间的差异，藉此说明影响叶序配列的因子。

It is highly valuable to genetic data storage, DNA sequences analysis, and genome phylogeny.

它对基因序列的数据储存、DNA的序列分析以及研究基因组的进化关系等方面都有着非常重要的意义。

3According to the movement point of discontinuity, divided the human body movement sequence into certain subsequences, thus effectively eliminated the ambiguous disturbance.

将人体运动序列按照运动突变点划分为若干子序列，从而有效消除二义性的干扰。

Then, a concept of time point in fuzzy temporal sequence is proposed with a fuzzy definition given to the tense relation attributes.

在经典时态关系的基础上，讨论了模糊时态区间关系，提出了时间点的模糊时态序列概念，并定义了模糊时态关系属性，同时用模糊时态序列定义了模糊时态区间的边界关系。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。