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The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白,本实验以美洲商陆夏季叶片为材料,通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增,对PAPⅡ进行了基因克隆、序列分析,结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9%,然后将该基因构建到原核、真核表达载体上,分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达,在两个表达系统中均获得了有活性的PAPⅡ表达蛋白,体外生物测定表明表达的蛋白均具有抑制病毒的活性,PAPⅡ基因在酵母中还没有表达的报道,这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

With a suitable performance function and an initial control that made the closed system bounded, a serial of controls that made the performances better could be obtained by policy iteration, and ergodic Markov chains were constructed by the state serial with the corresponding feedback control.

在合适的性能指标并能找到一个使系统性能有界的控制的前提下,通过策略迭代可以求出逐步改善系统性能的控制序列,同时得到状态序列在相应反馈控制作用下构成遍历的马尔可夫链。

We also use linear and polynomial fit to analyze their distribution trends and differences among chromosomes and among different kinds of sequences.

果蝇染色体参数X值是由D1和D2值共同决定的,但果蝇染色体的编码序列参数X分内含子序列相比,分布没有趋同性。

A truncated Volterra series expansion, whose polynomial number can be determined by error criteria, was used to model the biomedical signal component. Based on Volterra we proposed an adaptive filter that could approximate and extract the signal in noise corrupted ECG data efficiently.

利用误差准则确定的Volterra序列展开能很好模拟生物信号成份,而基于Volterra序列的自适应滤波器能有效模拟ECG成份从而达到去除信号中噪声的目的。

Aimed at some shortages in the existing methods based on piecewise polynomial representation, such as sensitive to noise awl lower computational efficiency, a robust method of optimal segmenting time series is proposed.

针对分段多项式回归方法存在计算效率低和对噪声较敏感等缺点,提出了具有稳健性的最优分割方法,以解决时间序列数据相似搜索及知识发现处理中的长时间序列分割问题。

Here the sequence of mRNA bases is translated into a sequence of amino acids in a polypeptide chain.

这里,mRNA的碱基序列被翻译成肽链里氨基酸的序列

The song complexity patterns of the white-rumped snow finch may belong to the "unstable changing in sequence of polysyllable" pattern with typical "serial song" from immediate variety of syllables.

依据雷富民等(2003)对鸟类鸣唱多样性和复杂性的评述,白腰雪雀鸣唱的结构模式符合"多音节序列不稳定变化型",其鸣唱曲目中音节的转换形式为"序列鸣唱"。

Researchers who try to simulate human movement in artificial figures quickly discover what animators of Bugs Bunny and Porky Pig have known all along: that some linkage sequences are more "natural" than others.

那些尝试着要去用人工形象来模拟人的行动的研究者们很快就发现了那些制作兔巴哥和猪小弟的动画师们早就知道的事情:有些联接序列会比其他联接序列显得更"自然"。

Within a Pragma, the character content may consist of any sequence of characters that does not include the sequence "::".

在一个附注里,字符内容可以有任意字符序列组成,其中不含序列"::"。

In order to further test the implemental effects of the new rule on earning preannouncement we consider to make our systematic analysis of the stability of the stock market in terms of the fluctuation.

我们尝试利用波动率数值的时间序列表现进行研究,力图了解:①预告公司股票日收益率序列的时变条件方差是否满足稳定性要求。

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Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。