孵

In present study, we used the adult rabbits articular chondrocytes as the object of study, we researched the effect of TGF-β1 to promote chondrocytes proliferation and preserve the synthetise of special stroma in order to provide theortically and clinically basis of culture and proliferation in vitro of a-dult people articular chondrocytes.

材料与方法 1。成年兔关节软骨细胞分离、培养：取18月龄的成年日本大耳白兔1只，处死，片状切取关节软骨，解剖刀将软骨切成1.0mm~3大小碎块，消化，过滤，离心，收集关节软骨细胞，加入DMEM培养液(含20％胎牛血清、青霉素100U／ml，链霉素100U／ml)，调整细胞密度为2.5×10~5／ml，置于37℃，5％CO_2孵育箱内培养。

Of 11 chicks hatched since July, seven have been killed by stoats and cats.

在自7月份孵出的11只幼鸟中，有7只被白鼬和猫吃掉了。

The test system is incubated at 37℃for 1 hour prior to the addition of 25 μl of 1.4% standardised sheep red blood cells in VBD sensitised with 5 units of rabbit anti-SRBC.

37℃孵育1小时后加入25微升已致敏的含5个单位的兔抗绵羊红细胞，用VBD液稀释成1.4%的标准绵羊红细胞

Fred opened an "egg carrier" box and divided up fragmentation and concussion grenades into three satchels.

佛瑞德打开一个绰号&孵蛋器&的盒子，把杀伤手雷和震荡手雷分开装进三个小背包。

His chieftain had already remarked on the runts produced in the last batch, although even a runt of a dragon stood several times higher than an orc.

这够让他头痛的了，老萨满酋长总是提起上一窝孵出的幼龙，尽管龙刚破卵而出就比兽人高好几倍。

The shell uses the pearl that own tears hatched to teach the beauty, he do like this be for causing the other people's attention!

贝壳用自己的泪水孵育了美丽的珍珠，他这样做就是为了引起别人的注意呀！

Then the HCV subgenomic replicon cell model was incubated with Napp at concentration of 1mg/ml, 10~(-1)mg/ml, 10~(-2)mg/ml, 10~(-3)mg/ml, 10~(-4)mg/ml in cell culture solution and the negative and positive controls were set up correspondingly. After 3 days, the intracellular RNA of HCV subgenomic replicons were detected by FQ-RT PCR.

然后分别取终浓度为1mg/ml、10~(-1)mg/ml、10~(-2)mg/ml、10~(-3)mg/ml、10~(-4)mg/ml的Napp与含HCV亚基因复制子细胞培养液中共同孵育，同时设空白对照组和IFN-α2b阳性对照组，3天后用荧光定量PCR法检测HCV复制子中RNA的含量。

Hatching pheasant, duck, partridge, peacocks, red-crowned crane, the Golden Rooster, birds hatching automatic machine, Chu Chu machine, hatched dual-use machine for special poultry market, zoos, scientific research units.

孵化山鸡、野鸭、鹧鸪、孔雀、丹顶鹤、金鸡、鸟类全自动孵化机、出雏机、孵出两用机，适合特禽场、动物园、科研单位使用。

The previous month, you have picked a free electric refrigerator, day before admitted an egg, second days hatched six pullets.

上个月，你又捡了一个免费的电冰箱，头天放进一个鸡蛋，第二天孵出六只小母鸡来。

B:Oocytes and early embryos were permeated in 0.5%Triton X-100 for 10 minutes,then fixed in 4% paraformaldehyde/PBS.Method C:Oocytes and early embryos were fixed in 1%PFA while permeated in 0.2%Triton X-100 at the same time for 1 hour.Method D: Oocytes and early embryos were fixed in precooled methanol(-20℃) for 15 min,then permeated in 0.5%Triton X-100 for 10 minutes.

方案A：先用4%多聚甲醛固定1h，再用0.5%Triton X-100穿透10min；方案B：先用0.5%Triton X-100穿透10min，再用4%PFA固定1h；方案C：用1%PFA+0.2%Triton X-100同时固定与穿透1h；方案D：用-20℃预冷甲醇固定15min，再用0.5%Triton X-100孵育10min。

He and Nina moved to California and lived at 2005 Ivar Street, Apt.

他和Nina搬到加州，并在2005年伊瓦尔街，公寓生活。

Droperidol （ potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道，可能是 QT 间期延长的机制。