基因

Rats living over 96h were considered survival. To observe the protective effects of ALR gene on acute liver injury rat, thirty-six rats that were injected peritoneally with 50% CCl4 of 2ml/kg were divided into the flowing 6 group according to doses (50?g/kg and 200?g/kg ) and administration routes of pcDNA3-ALR DNA 4h after CCl4 injection: group 1, model group; group 2, ALR gene of 50μg/kg was injected venally; group 3, ALR gene of 200?

另取36只大鼠按2ml/kg腹腔注射50% CCL4，染毒后4h按不同剂量（50μg/kg 和200μg/kg ）、不同注射途径（尾静脉注射、腹腔注射和联合注射）随机分为六组。1组：模型组，不注射ALR基因； 2组：尾静脉注射ALR基因 50μg/kg； 3组：尾静脉注射ALR基因 200μg/kg； 4组：腹腔注射ALR基因 50μg/kg； 5组：腹腔注射ALR基因 200μg/kg； 6组：联合注射（尾静脉和腹腔各注射ALR 基因100μg/kg）。

Our previous studies demonstrated that the IBV was easy broken, and the virus coronal was easy lost When IBV was isolated from the allantoic fluids of eggs, so It was hard to harvest the whole IBV antigen.

克隆了IBV M41毒株N基因和IBV H52毒株S1基因，对它们进行了序列测定和与GenBank中收录的IBV N基因、S1基因序列进行了比较分析，并用Clustal X和Mega软件将N基因、S1基因与公布的标准序列做同源性比较和系统进化分析。

At present, regeneration system of cotyledons, hypocotyls, and stems, protoplasts and cotyledons codes have been established among Cucurbitaceae. There are several methods of transformation, such as agrobacteria -medidated genetic, particle gun, pollen tube passage, DNA soaking, ovary embryonary sac injection. Transgenic is tending to antivirotic and delayed maturity. Selection marker used often is NPT II.

目前，在葫芦科植物中已建立了子叶、茎尖、胚轴、茎段、原生质体和子叶节等的再生体系；用于遗传转化的方法有农杆菌介导法、基因枪法、花粉管通道法、DNA浸泡法等；用于转化的目的基因多为抗病毒病基因和延迟成熟的基因；NPTII基因为最常用的筛选标记基因。

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具，分别以人丁酰胆碱酯酶(1p0i：A)和果蝇乙酰胆碱酯酶(1d×4：A)的蛋白晶体结构为模板，对嗜虫书虱2个AChE的三维结构进行同源建模，并在三维结构中发现了AChE的酶解活性位点，证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少，在GenBank中没有可用作内参基因的序列，因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号：FJ041117)，该片段长度为822 bp，编码273个氨基酸残基，同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

Based on these observations, we predict that the transmission of GA-signaling genes occurs in a sporophytic manner, since the protein products and/or mRNA transcripts of these genes may be introduced into pollen-carrying mutant alleles, whereas GA synthesis genes are transmitted in a gametophytic manner, since these genes are preferentially expressed after meiosis.

基于这些观察，我们预测该遗传算法，基因信号传输时，在孢子的方式，因为蛋白质的产品和/或基因这些基因的转录可以引入花粉携带突变基因，而合成的基因遗传传递以配子体的态度，因为这些基因是优先减数分裂后表示。

Plant traits were categorized to three classes: the first class traits were dominated by major gene with little genetic effect of polygene, including days from sowing to flowering, days from sowing to boll opening, lint percent, days from squaring to flowering, days from flowering to boll opening and plant bolls. The hereditability of major gene and polygene were about 36. 85%～66. 79% and 2. 83%～12. 64%, respectively.

通过对短季棉早熟及其相关性状的主-多基因综合分析发现：主基因普遍存在，各时期至少有一对主基因起主要作用，同时受多基因的修饰，在短季棉发育的不同时期，其主基因的对数、作用效果、方向以及主、多基因的遗传率均不同。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

Sequences of modified genes GOX and CP4-EPSPS in GM canola and that of modified gene Cryla in GM cotton were decoded and the conservative sequences of exogenous resistant genes: PLRVrep, PVYcp and CryⅢA in GM potato: New leaf〓 PLUS and New leaf〓 Y were confirmed using the modified exogenous gene decoding technique and isogenous sequence similarity BLAST analysis. Bases on DNA sequences studied above, conventional PCR primers were designed and selected in an optimized way and the conventional PCR detection protocol for exogenous resistant genes in 19 GM crops was established.

本研究采用反向PCR克隆—测序测定未知序列技术，针对转基因玉米（MON810、BT11、BT176、GA21、T25、CBH-351）六个品系、转基因大豆GTS 40-3-2品系、转基因油菜RT73和MS8两个品系、转基因棉花MON531和MON1445两个品系测定出品系鉴定的边界序列；采用外源修饰基因序列的破译方法和BLAST同源性序列分析技术，针对转基因作物中修饰的外源抗性基因进行破译研究，破译出转基因油菜中修饰的GOX基因、修饰的CP4-EPSPS基因和转基因棉花中修饰的Cry1A基因的序列，并确定了转基因马铃署New leaf〓PLUS和New leaf〓Y两个品系中PLRVrep、PVYcp和CryⅢA外源抗性基因保守序列。

Moreover, CoI gene was more conservative and a lower evolutionary rate than Cyt b gene. CoI gene was an effective marker in analysing the phylogenesis of families in Passeriformes. It can be used in identifying the species of Passeriformes, but it was revealed that CoI gene was more suitable to identify the phylogenetic relationship of avian family unit than Cyt b gene, and CoI gene can be a molecular marker to identify avian species. But in species identification, CoI gene was less stable and accurate than Cyt b gene. We suggested youd better employ the other marker if you have the CoI gene only.(2) In the phylogenetic trees of birds from Lanius, L.

比较分析了雀形目6科15种鸟类的细胞色素b全序列和CoI基因部分序列，结果显示细胞色素b和CoI基因序列的变异位点分别为454个和366个、简约信息位点为337个和303个，而且线粒体CoI基因比细胞色素b基因略微保守、进化速率也较低；CoI基因在确定雀形目科级阶元之间的系统发生关系方面是一种有效的分子标记，同时它也能够用于雀形目鸟类的物种鉴定，但在物种鉴别方面不如细胞色素b基因稳定、准确。

Was simultaneously injected into cisterna magna at the second injection blood in the presence of subarachnoid blood. At 2 to 4 days, GFP was expressed in leptomeninges over the brain stem, cortex and cerebral arteries, smooth muscle cells of small vessels were occasionlly transduced. GFP was expressed in adventitia of spastic basilar artery on days 2 (day 5 after first injection blood) after injection 〓, but was undetectable by days 4, transgene was not expressed in medial or intimal layers. The prensence of subarachnoid blood can not prevent access of adenovirus to vessels and transgene expression.

注入腺病毒载体后2天（初次注血第5天）行荧光显微镜、免疫组织化学和RT-PCR检测，结果在颅内大血管如基底动脉的外膜可见外源基因的表达，而外源基因不能转移至血管中膜和内膜，4天时（初次注血第7天）基底动脉的外膜中外源基因表达消失；注入腺病毒载体后2～4天，外源基因可以有效转移至颅底软脑膜细胞，小血管外膜和平滑肌层也可见外源基因的表达，表明蛛网膜下腔内的血凝块不能阻止腺病毒载体介导外源基因转移至颅内血管。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。