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To observe method of chick embryo allantois membrane, judge neovascularisation, research the effect of angiogenesis by drugs by means of chick embryo allantois membrane model.

学会观察鸡胚尿囊膜血管的方法,判断新生血管形成,通过鸡胚尿囊膜模型探讨药物对血管生成作用的影响

There are commonly eight nuclei in the embryo sac: the egg apparatus at the micropylar end, made up of an gee nucleus and two synergid nuclei; three antipodal cells at the oppsite chalazal end that probably aid embryo nourishment; and two polar nuclei in the center that fuse to form the primary endosperm nucleus.

通常胚囊中有8个核,卵器在珠孔端,由卵和两个助细胞组成;3个反足细胞在合点端,可能为胚胎提供营养;胚囊中央有2个极核,融合在一起形成最初的胚乳核。

The mature embryo sac has 7 nucleuses in solar greenhouse.

在日光温室条件下形成的成熟胚囊都是7核胚囊。

Nucellar cells degenerated in advance under low temperature, which resulted in thickened embryo sac wall surrounding the megasporocyte.

低温处理的蝴蝶兰,胚囊发育过程中珠心细胞提前退化,在大孢子母细胞外围形成较厚的胚囊壁。

In the late of April of the second year, male inflorescences unbound and pollinated. When female inflorescences accepted the pollen grains, ovary development was increased in early May. With the development of either of the two ovule, funiculus, integument and nucellus were differentiated. Embryo sac mother cell came into being and the meiosis begins. Then fuctional megaspore developed into matured embryo sac that contain eight nucleuses. In the end of May, double fertilization generated.

次年,4月下旬,雄花序解螺旋散粉,雌花序开花授粉;5月上旬,雌花序授粉后,迅速增长,子房膨大,两个胚珠分化出珠柄、珠被和珠心,珠心中产生孢原细胞;5月中下旬,大孢子母细胞形成并减数分裂,功能性大孢子形成成熟的八核胚囊;5月底,发生双受精。

The mature embryo sac fertility, formation and development of embryo sac of 141 accessions of O. rufipogon Griff. collected from the six sampling sites in Gaozhou, Guangdong Province were studied by using the technology of whole-mount stain-clearing confocal laser scanning microscopy.

利用整体染色激光扫描共聚焦显微镜术,对采自广东省高州市6个地点共141个编号的高州普通野生稻的成熟胚囊育性和胚囊形成发育特点等进行研究。

Histological structures of bursas of Fabricius were observed at the different developmental stages from chicken embryoes to chickens. The results were as follows. The bursa of Fabricius began to develop on the 13th day of post-hatching and its cell aggregated into the nodosity clique underneath the epithelium mucosae. On the 15th day of post-hatching, those cell cliques were surrounded by the flat epithelium and formed the basic structure of folliculus lymphaticus. The number of lymphocytes in the folliculus lymphaticus increased gradually, and the medulla and cortex of a majority of folliculus were not discriminated obviously. Until on the 7th day for chickens, the medulla and cortex of folliculus formed completely and the number of folliculus increased significantly. From 21 to 35 days old, the folliculus lymphaticus transformed from polygon into rectangle. Mucosa epithelial cells increased gradually and their cytoblasts arranged compactly into aline in the basilar part on the 35th day.

本研究观察了从鸡胚到出壳后的不同生长发育阶段法氏囊的组织结构,结果表明:胚胎13日龄时,法氏囊开始发育,粘膜上皮下形成结节状细胞集团;胚胎15日龄,聚集的细胞集团形成初始的淋巴滤泡;胚胎18日龄到出壳后1日龄,滤泡内淋巴细胞数量增多,多数滤泡髓质和皮质界限仍不明显;7日龄雏鸡法氏囊髓质皮质分界清晰,滤泡数量显著增多;21日龄到35日龄,滤泡由多边形逐渐转变成长方形;在35日龄时,粘膜上皮细胞逐渐增多,排列紧密,细胞核紧密排列成直线位于基底部。

Could be observed on 10th day after pollination, archesporial cell on 20thday, megasporocyte on 24th day, the functional megaspore on 30th day; and 8-nucleate embryo sac on 41th day. And then double fertilization finish and early globular embryo emerg.

授粉10 d出现指状突起,20 d形成胚珠原基,24 d大孢子母细胞发生,30 d功能大孢子形成,41 d发育为成熟的八核胚囊,而后完成双受精发育成早期球形胚。

The nucleus of mature egg cell was located at the micropylar end and a great deal of starch grains were in the cytoplasm surrounding the nucleus.

胚囊发育为蓼型,反足细胞分裂形成反足组织,反足组织含有大量淀粉粒,反足细胞直到胚乳细胞化时才退化,反足细胞在胚和胚乳发育中起着积极作用。

Megaspore mother cells undergo meiosis, which results in the formation of 4 megaspores in a row. The chalazal-most cell develops into an 8-nuclei embryo sac after 3 rounds of mitosis. A mature embryo sac is produced after cellularization and nuclear migration.

结果表明:1大孢子母细胞经过减数分裂形成直线排列的4个大孢子,其中珠孔端的3个大孢子退化,合点端的大孢子进行3次有丝分裂,形成八核胚囊, 8个核迅速细胞化,最终发育为成熟胚囊。

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