囊胚形成

Megaspore development and abortion of"Pingyi Tiancha"Compar-ing with regular developing megaspore of normal M.baccata,the megasporemother cellof"Pingyi Tiancha"could form mono-nucleus embryo sacby perhaps irregular meiosis,then most of sexual MES depauperatedand degenerated,finally aborted,or only a few further developed.Of asexualembryo sac,one or more large cells at the chalazal end of nucellus developed in-to one to more asexual embryo sac initials.After the competition between sexu-al embryo sacand asexual embryosac,most ovules only had one asexual embryo sac matured.

平邑甜茶大孢子的发育和败育与正常发育的山定子大孢子对比，平邑甜茶大孢子母细胞经减数分裂能形成单核胚囊，之后多数有性胚囊萎缩、退化而败育，只有极少数进一步发育；而无性胚囊则从胚珠合点端1个或多个较大的珠心细脆形成并首先发育为一个或多个无性胚囊原始体；经过有性胚囊和无性胚囊以及无性胚囊和无性胚囊之间的竞争，多数胚珠只有一个无性胚囊成熟。

"In mammal s, the fertilized egg or zygote undergoes cleavage (cell division without cell growth) to form a hollow ball or blastocyst."

在哺乳动物中，卵与精子结合而成受精卵，受精卵发生数次细胞分裂后，形成一个中空球形的囊胚。

"In mammals, the fertilize d egg or zygote undergoes cleavage (cell division without cell growth) to form a hollow ball or blastocyst."

在哺乳动物中，卵与精子结合而成受精卵，受精卵发生数次细胞分裂后，形成一个中空球形的囊胚。

"In mammals, the fertilized egg or zygote undergo es cleavage (cell division without cell growth) to form a hollow ball or blastocyst."

在哺乳动物中，卵与精子结合而成受精卵，受精卵发生数次细胞分裂后，形成一个中空球形的囊胚。

Mouse blastula showed clear band in 24-72 hours, came out from cell membrane in 1 day. After 3-5 days, it become embryonic stem cell mass. At 7 days, the cell mass showed "bird nest" shape.

小鼠囊胚孵出透明带的时间为24~72 h，从胞膜中孵出约为1 d，3~5 d后可形成胚胎干细胞集落，7 d后集落呈&鸟巢&状。

These orient themselves so that a fluid-filled cavity, the blastocoel, forms inside the mass of cells

这些细胞进行自我调整，以致在细胞群内部形成一个充满液体的腔，即囊胚腔。

The embryo sac developed into functional megaspores only and no mature embryo sac was generated.

胚囊只发育到单核胚囊阶段，没有形成成熟胚囊。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明：(1)水牛精子胞质内显微受精的雌、雄原核发育不同步，雌原核比雄原核早3h形成；(2)水牛卵母细胞ICSI和激活后用1.9mmol／L 6-DMAP培养处理3h能提高其胚胎发育率；(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率；(4)死精子能用于水牛卵母细胞的ICSI；(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Subsequently cellular become divided brings about a series of " kind the formation of bursa embryo ".

随后的细胞分化则导致一系列&类囊胚&的形成。

He and Nina moved to California and lived at 2005 Ivar Street, Apt.

他和Nina搬到加州，并在2005年伊瓦尔街，公寓生活。

Droperidol （ potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道，可能是 QT 间期延长的机制。