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Common buckwheat ( F. esculentum ) is an allogamy crop, which is heterogony. There is heterogeneity in the species. To reveal the genetic diversity within cultivar population, In order to explore the sampling strategy that can reveal genetic diversity of buckwheat resources on the molecular level. PCR with intron-splice junction primers and long random primers was employed to reveal the genetic diversity within nine common buckwheat varieties and one tartary buckwheat cultivar population among different individuals. We established the sampling strategy in its genetic diversity study. Then we used the established sampling strategy, and the same PCR markers technique,to analysis the genetic diversity of 86 common buckwheat resources, the main results are as follows

甜荞是花柱异长自交不亲和的异花授粉作物,品种内存在一定的异质性,为了准确揭示甜荞遗传资源的遗传多样性,本研究采用因子分析法对86份甜荞遗传资源农艺性状数据进行分析;采用长随机引物和内含子切接点引物的PCR分子标记技术,对9个甜荞品种和1个苦荞品种不同个体间的遗传多样性进行了分析,建立了甜荞资源遗传多样性研究的取样策略;并利用建立的取样策略,采用PCR标记技术,对这86份甜荞资源的遗传多样性进行了分析,取得如下主要结果

In all 22 loci detected, 18 and 11 loci are polymorphic between and within cultivar, respectively.

所使用的SSR分子标记共可侦测到21个基因座,其中有18个基因座在品种间表现多型性,有11个基因座在品种内表现多型性。

The main chemical compositions of tea flower were studied. It is the first time to determine the levels of protein, reducing saccharides and SOD activity in different blooming stages of tea flowers collected from three cultivars of Camellia sinensis with different picking time. Results showed that there were significant differences among cultivars and blooming stages, as well as the change of these chemical constituents along with time. Consequently we selected the right cultivar that has high protein, reducing saccharides or SOD activity and the fitting deflower time.

本文研究了茶花中主要化学成分的含量,首次对不同茶树品种、开花状态和采摘时间的茶花中蛋白质、还原糖含量,以及SOD活力进行系统研究,明确了不同茶树品种、不同开花状态茶花中蛋白质、还原糖含量和SOD活力的差异,以及这些化学成分随时间的变化规律,从而筛选出了高蛋白质、还原糖含量,以及高SOD活力的茶树品种和适宜的采花时间。

After pollinated 50days the result indicated that fruit shape index weight of single fruit and volume of single fruit on Suli pear variety during fruit growth were significant different because of pollex xenia action of some of different varieties pollinating on Suli pear variety .

选用当前生产中推广的8个梨优良品种给酥梨授粉,授粉后50d调查分析,结果显示:由于授粉品种的花粉直感作用,使得酥梨果实的果形指数,单果重及体积发生变化,一些品种间存在着显著差异。

In the low B condition of the soilless culture, rapeseed seedlings were short of B, the seedlings were short and the leaves were malformed and the weight of the shoots decreased, the branch roots were fewer and snuff color, the weight of roots decreased, and the length of root and the ratio of shoot/root decreased, too. However, the high B- efficiency type of rapeseed had the a great ability to the low B stress, the growth of shoots and roots were better than the low efficiency, and the B concentration range of the rapeseed varieties which responded to the B scarce was 0.001 mg/L to 0.01 mg/L.

营养液培养条件下,苗期缺硼,甘蓝型油菜植株瘦小,叶片皱缩,地上部生长量下降;侧根少且呈现黄褐色,根重、根体积、根长以及根冠比下降;高效品种具有较强的耐低硼胁迫的能力,表现为同等缺硼条件下,对高效品种地上部和根系生长的抑制作用都相对较小,且品种间对缺硼反应差异最大的硼浓度范围为0.001mg/L—0.01mg/L。

To investigate the relationships between the tolerance difference to high temperature stress and superoxide dismutases in different cultivars of Celosia cristata, ten-leaf stage seedlings of the heat-tolerant cultivar, Century Green and the heat-sensitive cultivar, Century copper were pulsed for 48 h with diethyldithiocarbamate pretreatment and subjected to high temperature stress under controlled conditions of relative humidity of 75%~90%, 12 h/24 h photoperiod with 1 500 lx and 45 ℃, to research the changes of external morphology and interior physiology.

李永红;魏玉香深圳职业技术学院应用化学与生物技术学院,深圳 518055摘要为研究鸡冠花品种间耐热差异与超氧化物歧化酶之间的关联,选用鸡冠花耐热品种世纪绿叶和热敏品种世纪铜叶的10叶期幼苗为试材,用二乙基二硫代氨基甲酸钠进行48 h预处理之后,在45 ℃、相对湿度75%~90%,、光照1 500 1x (12 h/d)条件下进行热胁迫处理,研究其形态和生理生化的变化。

The variation rates of four wheat lines were 2%~3%. The characters of SP1 variation plants at maturity stage also appeared big changes. SP1 variation plants had short growing period, high seed setting rate, loose plant-type and clavated spike-type. There were not consistent change in plant height, the number of spike grain and the weight of 1000 kernel among four lines.

四个小麦品种SP1代变异株率为2%~3%;成熟期SP1代变异株性状也有较大变化,表现为生育期显著缩短,结实率有所提高,株型向松散趋势发展,穗型则均表现为棍棒形,株高、穗粒数和千粒重的变化品种间表现不一致。

The suppling K increased the LAI,LWR and RGR,but there was no obvious difference between K treatments.

施钾处理能增加LAI和SWt,提高R1后的平均RGR,但不同钾处理间未达显著水平。R1时两处理间和不同品种间K%和VKUE均达极显著差异。

In addition, genetic diversity of landraces was greater in Sichuan than in the other three regions.

玉米地方品种内遗传多样性及品种间遗传距离分析结果表明,在我国西南地区,分布在四川的玉米地方品种具有最丰富的遗传变异。

The effect of low temperature treatment on DNA methylation level decrease revealed some variety differences. The DNA methylation level in stem apices of the spring radish cultivar'Duanye 13'was somewhat easy to be decreased by low temperature treatment, but that of the winter radish cultivar'Yidianhong'did not decrease when the germinated seeds were treated at 10℃ for 10 days.

萌动种子低温处理降低DNA甲基化水平的效果在品种间存在一定差异,春性品种'短叶13'其DNA甲基化水平比较容易被降低,而冬性品种'一点红'的萌动种子在10℃下处理10d尚不能降低其DNA甲基化水平。

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