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The average interspecific divergence was 3.860%, and special sites were found in each chicken breed which was the bases to identifying chicken breeds. The DNA taxonomy of Bar1 used NJ was consistent with morphological taxonomy among the six indigenous chicken breeds. However, the analysis of Bar2 sequences of COⅠindicated that they were lower polymorphism and no special site in most chicken breeds which was not benefit to identify chicken breeds. The DNA taxonomy of Bar2 used NJ was not consistent with morphological taxonomy among the six indigenous chicken breeds.

本研究选择的2段序列中,以Bar1序列648bp(712位点到1359位点)多态性最高,突变位点最多,为24个,品种间平均多态性为3.860%,6个鸡种在此序列中有各自不同的特异位点,NJ聚类分析6个品种基本被聚为不同的类别,与形态学分类基本一致,该COⅠ基因的Bar1序列用于这些品种鉴定是可行的;而Bar2序列进行序列多态性分析,由于其多态性低,大多品种没有其特异位点,NJ聚类分析6个品种基本被聚为不同的类别,与形态学分类基本一致,不利于品种鉴定。

It showed that the halophile of Kenyu7 were better than Wuming-5;(2)the contents of proline of the two Zea mays varieties increased significantly,and under high NaCl stress(150mmol/L NaCl or above) the increasing amount of the contents of proline of Kenyu7 was higher than Wuming-5 ,so it showed that the injuries of Wuming-5 imposed by NaCl stress was greater than Kenyu7;(3)the activities of CAT of the two varieties were no obviously changed,while the activities of CAT of Wuming-5 were all higher than Kenyu7 under all concentrations of NaCl solution,which hadn't showed the correlation between the activity of CAT and salinity tolerance.

结果表明,随NaCl溶液浓度的增加:(1)两品种玉米生长均受到严重抑制,发芽势降低,芽和根的长度均呈减小的趋势,总体来看,杂交种垦玉7的发芽势、芽和根的长势均好于自交系无名-5;(2)两品种玉米体内Pro含量均增加,且在NaCl浓度超过150mmol/L时,杂交种垦玉7体内Pro含量增加的幅度明显大于自交系无名-5,而使垦玉7对盐的适应能力强于无名-5;(3)两品种的CAT活性变化幅度均不大,但无名-5的CAT活性在各NaCl浓度条件下均高于垦玉7,这可能是由品种间本身差异造成的,而与品种的耐盐性无直接关系。

In this study,two SNPs of chicken FASN gene were chosen to analyze genetic diversity and their associations with chicken growth and carcass traits by using a F_2 designed resource population.Results showed that:(1) In the PCR-RFLP site of HaeⅢ,allelic frequencies differed much among different chicken breeds,as B frequencies are the highest in Red Jungle Fowl(0.85),and the lowest in Taihe and Yugan Black(both 0.21).In the PCR-RFLP site of PstⅠ,A frequencies are the highest in Chongren Partridge(0.5),and the lowest in Naked Neck chicken(0.19),and the difference of gene frequencies was small among chicken breeds.

研究了脂肪酸合成酶(Fatty acid synthase, FASN )基因最后1个内含子2个突变的多样性,并运用资源参考家系研究了这2个突变对鸡体重、脂肪沉积性状的影响,结果表明:(1)红色原鸡Hae Ⅲ-PCR-RFLP位点B基因频率为最高(0.85),泰和鸡及余干乌黑鸡为最低(0.21),品种间差异较大,但未发现肉用与蛋用鸡种间的差异;在Pst I-PCR-RFLP位点上, A基因频率在崇仁麻鸡中表现为最高(0.5),在裸颈鸡为最低(0.19),品种间差异较小。

Heddewigii, Dianthus barbatus L, Dianthus latifolius, Dianthus caryophyllus and Dianthus chinensis var. liaotungensis, with relatively high fructification rates and certain traffic tolerance, showed little difference in ecological adaptability and exhibited an average seedling height of 20cm and a mean crown size of 23cm, and pruning during the late growth period could obviously promote flowering and reduce the seedling height by 3cm. While Dianthus barbatus L. and Dianthus phlox subulata, with relatively low fructification rates and higher traffic tolerance, had an unobvious difference in ecological adaptability, which present an average seedling height of 14cm and a mean crown size of 36cm, and pruning during the late growth period could promote flowering to some extent and reduce the seedling height by 2cm.

其中,大红石竹、锦团石竹、五彩石竹、五寸石竹、浓香石竹、重瓣石竹品种间差异小,平均苗高20cm,冠幅23cm,结实率相对高,有一定耐践踏能力,在生长后期进行修剪能很好的促进开花并降低株高3cm;常夏石竹和美国地被石竹品种间差异小,平均苗高14cm,冠幅36cm,结实率低,耐践踏能力稍强,在生长后期进行修剪能较好地促进开花并降低株高2cm。

In order to clarify the rules of POD isoenzyme in the hazelnut, identify their blood relations between female, male and filial generations and select the superior parents for cross-breeding, we have studied the characteristics of peroxidase isoenzyme which are distributed in the 34 hazelnut varieties or lines by polyarclamide gel electrophoresis, The results showed that:POD isoenzymes of Corylus heterophylla Fisch, Corylus avellana L, and the hybrid of Corylus heterophylla Fisch × Corylus avellana L had obvious difference; some hybrids had lost P10 spectrum band of parents; the enzyme spectrum of 32 hybrid hazelnuts produced 'new hybrid spectrum bands'that could be considered as a mean to identify hybrids.

为了阐明过氧化物同工酶在榛品种中的分布规律,探讨平榛、欧榛及其杂种榛之间的亲缘关系,选育优势杂交亲本,本试验采用聚丙烯酰胺凝胶垂直电泳,研究了34个榛品种成熟叶过氧化物酶同工酶酶谱特征,结果表明:平榛、欧榛及其平欧杂种榛品种间酶谱具有较大差异;有的杂种品种遗失了父母本共有谱带P10;32个平欧杂种榛品种出现了双亲所不具有的新酶带,可作为鉴别杂种的一条途径。

Seven grapevine cultivars originated from different regions, i. e.'Carbernet Sauvignon' and'Queen of Vineyard'(two members of the Western Europe Cultivars Group),'Rizamat'(a member of the East Cultivars Group),'Red Double Taste'(a hybridized cultivar from Vitis vinifera L. and V. labrusca L.),'1103Paulsen','Red Globe'and 'Autumn Royal'(two new hybridized cultivars between cultivars groups which were warmly planted in recent years) were selected. After different drought stress, a combined measurement of gas exchange and chlorophyll fluorescence was conducted to evaluate the effects of drought stress to photoinhibition, as well as the role of photorespiration and xanthophyll cycle in photoprotective mechanism. After photorespiration and xanthophyll cycle were separately inhibited, the complementary effect of these two photoprotective pathways was discussed. Detached leaves of different grapevine cultivars, i. e.'Cabernet Sauvignon','Cabernet Franc','Merlot', and a rootstock,'1103Paulsen'were selected as materials, and photoinhibition degree, photorespiration activity as well as its photoprotective function under drought stress were studied. The results are as follows: 1. Under the same drought stress treatment, different photoinhibition degrees formed in different grapevine cultivars.

本研究选择不同起源地的葡萄品种--包括西欧品种群的赤霞珠和葡萄园皇后、东方品种群的里扎马特、欧美杂种红双味、美洲种砧木1103Paulsen,以及近年来发展较快的品种群间杂交新品种红地球和皇家秋天等七个品种盆栽苗,在进行不同程度的干旱胁迫处理后,利用气体交换参数和叶绿素荧光参数同步测定的方法,研究干旱对其光抑制程度的影响,以及光呼吸和叶黄素循环组分在其光保护中的作用;并在分别抑制光呼吸和叶黄素循环的基础上,探讨了赤霞珠葡萄两种光保护机制的互补效应;同时选择不同的酿酒葡萄品种赤霞珠、品丽珠、梅鹿辄和砧木1103Paulsen的叶片,研究其离体干旱条件下光抑制程度、光呼吸活性及其光保护作用,结果发现: 1。

Libellula had different resistance to different races and Xiannong 4 was susceptible to all the races except CY18 and CY22. Resistant components had significant variances among different cultivars, races and cultivar×race. And Libellula had strong selection on different races, while the selection of Xiannong 4 was weaker than that of Libellula.

里勃留拉的抗性谱较宽,抗病性在小种间差异明显,而咸农4号对除CY18、CY22外的小种均感病;两者的抗病性组分在品种间、小种间以及品种×小种互作时均有显著差异;里勃留拉对不同菌系有较强的选择作用,而咸农4号的选择作用较弱。

Based on the genetic distance of 26% by SSR data, the cluster analysis with un-weighted pair-group method using arithmetic averages produced 2 major clusters: cluster Ⅰ of higher degrees of genetic differentiation and Cluster Ⅱ of lower degrees of genetic differentiation relatively, which are composed of 30 cultivars, and another cluster of much higher degrees of genetic differentiation, which is only composed of the cultivars of FHIA 25 and Pisang Jari Buaya.

依据26%的遗传距离,除了FHIA25和Pisang Jari Buaya单独化成1组外,其它30个品种可以分为2组:品种间遗传差异相对较高的组Ⅰ和品种间遗传差异相对较低的组Ⅱ。

The results were summarized by variance analysis and correlation analysis, the diversity reached significant positively correlated level of varieties carotene, polysaccharide,γ-am1nobutyric and crude fibre content, but the diversity bad- not reached significant positively correlated level of varieties Zn, Cr and Trigonelline content.

对11个品种肉用印度南瓜主要果实性状进行调查,并对果肉的8种营养成分测定;通过方差和相关分析得出结论:胡萝卜素、多糖、淀粉、γ-氨基丁酸、粗纤维含量在各个品种间差异均达到极显著,Zn、Cr、葫芦巴碱的含量在各个品种间差异不显著。

The results were showed that a high genetic diversity was observed using these molecular markers. Fifty-four Japanese plum cultivars were classified five groups from the dendrogram. The majority of cultivars of northern areas in China were clustered together, and cultivars of southern areas in China were clustered together. Therefore, the Japanese plum cultivars in China could be classified two groups: northern cultivars group and southern cultivars group.

结果表明:中国李品种间遗传相似系数较低,平均为0.451,从分子水平证明了我国中国李品种的遗传多样性很高;将供试中国李品种分为五类,其中第一类是原产我国北方地区的一些品种,第二是原产我国南方地区的一些品种;第三类是从国外引进的品种,并提出了将我国的中国李品种分为北方品种群和南方品种群观点。

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