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RFLP and AFLP could be employed to make the linkage map and gene tagging by using the change of base pairs and the molecular rearrangement in restriction sites in specific restriction sites of DNA.

RFLP和AFLP技术利于花生图谱构建,利用DNA中特定的限制性酶切位点上碱基对的改变及酶切位点之间的分子重排,可以发现花生品种间的DNA许多多态性位点,进而绘制分子标记图谱。

The results showed that high-concentration salt solution restrained significantly sprout rates of these varieties.

结果表明:高浓度盐溶液显著抑制水稻种子的发芽率,但品种间有较大差异;筛选出苗期极端耐盐品种6份,其中1级1份,2级4份,3级1份;极端耐盐水稻品种苗高的增长受高浓度盐溶液抑制,但品种间差异不显著;而盐溶液对极端耐盐水稻品种出叶速率的影响因品种而异。

Investigation of the insulin-like growth factor system in breast muscle during development in Langshan and Arbor Acres chickensWe analyzed the effects of nutrient availability on the breast muscle insulin-like growth factor system.

本研究揭示了在生长轴、甲状腺轴和肾上腺轴中某些基因在特定时期在转录水平上的表达是相关的,并且发现下丘脑、垂体和肝脏中某些基因在品种间的表达差异是受营养因素调控影响的。2。

This paper has studied the physiological and biochemical changes during the period of forming strong buds of tree peony, and expect to provide academic basis for extending its view value and economic benefit . The main results are as the following:1 Under the field condition, Characteristics of photosynthesis of tree peony are very regular. From before blooming to the prophase of abducting buds that after blooming ,that is April,May and June,the net photosynthetic rate of tree peony is higher than the other period .It indicates that this period is very important for accumulating photosynthetic products of tree peony . So, if we want to get quality flower of tree peony ,it is very important to strengthen the management of tree peony cultivation and to ensure provide the fertilizer and water.2 From the middle ten days of June, with the time elapsing and with the environment conditions worsening, the characteristics of photosynthesis of tree peony decline deeply. But it is different among different tree peony breeds. For example , cv. Wu Long Peng Sheng can also has higher net photosynthetic rate, it indicates that cv. Wu Long Peng Sheng is super than the other two tree peony breeds when adapting the environment conditions and cv. Wu Long Peng Sheng has wide prospect to extend .3 The main factors of effecting the characteristics of photosynthesis are stomatal factor and non-stomatal factor ,that is the ability of photosynthesis of leaf cells. Under the environment of high light intensity in midsummer, if overshadow tree peony properly, then it can slow the declining of chlorophyll content, and increase the net photosynthetic rate and benefit for the accumulating of photosynthetic products.4 The content of soluble sugar in the root of tree peony is the highest among different organs during the period of forming strong buds . According to analyse ,we believe that the soluble sugar will compose starch then, and provide the solid base of material for the growth and development next year. It provide us strong academic basis of adopting the method of cultivating root and protecting root under thefield environment conditions .In other words ,it will help to increase the health level of the whole plant of tree peony and will be beneficial to forming quality tree peony flower if we strengthen the management of root. 5 To tree peony, the endogenesis hormone is important for its growth and development .In the prophase ,that is about the blooming of tree peony and the mid-summer ,IAA and ZR contents are higher and GA content is lower and its changes are very little, and the content of ABA is decreasing . This indicates that IAA and ZR act the important promote function for growth and development of tree peony in this period, and the function of GA is not important. Bur in the period of anniversary senescence of tree peony , the content of IAA and ZR begin to decrease ,while the ABA content has a certain rebound .T

本研究对几个牡丹品种壮芽形成过程中的某些生理生化变化做了研究,以期了解牡丹生长发育过程中生理生化的变化规律,探讨大田栽培条件下提高牡丹花质量的栽培措施和管理技术,为提高牡丹的观赏价值和经济效益提供理论依据,结果如下:1 大田栽培条件下,牡丹光合特性在生长发育过程中表现出极强的规律性,牡丹各品种的光合速率从牡丹开花前,到牡丹花谢后的花芽诱导期前期(6月10日之前),也就是在4、5、6月份,各牡丹品种的净光合速率相对与其他时间都维持在比较高的水平,这说明,在这段时间,是牡丹光合产物积累的重要时期,因此,要保证得到高质量的牡丹花,在这段时间加强对牡丹的栽培管理,保证这个时期的肥水供应十分重要。2 从6月中旬后,随时间的推移,环境条件的恶化,牡丹的光合性能剧烈下降,但是不同牡丹品种间仍有差异,比如,乌龙捧盛仍可维持较高的光合水平,这说明了乌龙捧盛这个牡丹品种对于环境的适应性较之其他牡丹品种高,有着广泛的推广前景。3 影响牡丹光合性能的主要因子是气孔因素和非气孔因素即叶肉细胞的光合能力,在盛夏高温高光强的环境条件下,对牡丹进行适当遮荫,能缓解牡丹叶绿素水平的下降,适当提高Pn,有利于光合产物的积累。4 可溶性糖在牡丹各品种的壮芽形成过程中,根中可溶性糖的含量在牡丹各部位中始终维持最高水平,分析认为这些可溶性糖在根系中进一步合成淀粉等储藏性物质,为来年牡丹的生长提供雄厚的物质基础,这为我们在大田栽培条件下对牡丹采取养根护根的栽培措施提供了有力的理论依据,即在大田栽培条件下,加强对牡丹根系的管理,有助于提高牡丹整株的健壮程度,有益于形成高质量的牡丹花。5内源激素对牡丹的生长发育调节作用是十分明显的,在前期,即开花前后和盛夏,IAA和ZR含量维持在比较高的水平,GA含量低且变化不明显,ABA含量逐渐降低,说明了IAA和ZR对牡丹在这段时间内的生长发育起到重要的促进作用,GA的作用不是很明显,ABA可能在高温高光强下有明显的升高,也说明了ABA的确有一些促进作用,但是到了牡丹周年衰老期,IAA和ZR的含量开始下降,ABA含量却有了一定程度的反弹,说明了IAA和ZR的作用开始降低,ABA起到了促进衰老的作用

When the aphids were feeding on Ww2730 seedlings, the time to 1st probing was delayed compared to those on Xiaoyan22 and Batis, searching was interrupted before 1st probing, and 1st probing was shorter than that on Xiaoyan22 and Batis significantly. Duration of pd Ⅱ-1 on Ww2730 and Xiaoyan22 was much longer than that on Batis. Duration of pd in C waveforms on Ww2730 was longer than that on Xiaoyan22 and Batis remarkedly. Times and duration of G waveform on Ww2730 were more and longer. Times of spot G waveform on Batis were more than on Ww2730 significantly, but the duration was shorter than those on Xiaoyan22 and Ww2730. Total time of E1 waveform, max longest duration of E1 fractions and mean duration time of E1 fractions following E2 wavefrom on Xiaoyan22 and Batis were all longer than on Ww2730 statistically. There were no differenences among mean durations of 1st E1 wavefrom on three varieties, but mean durations of other E1 waveforms on Ww2730 and Xiaoyan22 were shorter than those on Batis. The other waveform parameters, include times and durations of F and E2 were all not different on three wheat varieties.It is suggested that the resistance mechanism of wheat variety Ww2730 to S.

麦长管蚜在品种Ww2730上口针开始接触叶面时间显著晚于小偃22和Batis,第1次持续刺探前的刺探次数显著多于小偃22和Batis,开始刺入叶面后第1次刺探的持续时间极显著短于小偃22和Batis;在Ww2730和小偃22上的PdⅡ-1的平均时间显著长于Batis;Ww2730上C波阶段2个Pd波间的间隔平均时间显著大于另外2个品种;在Ww2730木质部取食的次数和时间显著大于其它2个品种;Batis上的点G波的次数极显著地高于Ww2730,而平均持续时间极显著短于小偃22和Ww2730;在Batis和小偃22上E1波的总时间,E1波的最大值、跟随E2波的E1波的平均时间均显著大于Ww2730;3个品种间第1次E1波持续的时间差异不显著,但Ww2730和小偃22上其它E1波持续的时间极显著少于Batis;3个品种上其它波形参数,包括F波的次数和总时间、E2波的次数和总时间没有差异。

Thirty-eight collections of Chinese orchids in Yunnan Province, China, including 14 species and 3 varieties, were analyzed phylogenetically using AFLP technique.

采用AFLP技术对云南省兰属的14个种和3个变种中的38份样本进行了亲缘关系分析。16对AFLP引物扩增的条带聚类结果表明,云南省国兰遗传多样性非常广泛,相同品种间的不同个体扩增出的条带模式相差很大。

Analysis of nutritional components in pear fruits of different varieties showed that fructose, glucose and sucrose were predominant sugar in pear fruits, the content of which ranged from 3.40% to 9.24%, 0.21% to 2.72%, 0.10% to 1.69%, respectively; Content of titrable acid was the highest in Bayuehong Pear(0.286%) and lowest in Atago(0.078%); Content of Vitamin C was the highest in Beurre Bosc (9.50 mg/100g) and lowest in Lubaoshi Pear(1.89mg/100g); Content of protein ranged from 127 to 428 mg/100g, content of free amino acid ranged from 24.8 to 77.4 mg/100g; Contents of the same mineral element of different pear varieties were different.

不同梨品种果实营养成分含量的分析结果表明:果糖、葡萄糖和蔗糖是梨果实中的主要糖类,品种间三种糖的含量有较大差异,所测品种果实中三种糖的含量范围分别为:3.40%~9.24%、0.21%~2.72%、0.10%~1.69%;可滴定酸的含量以八月红最高(0.286%),爱宕最低(0.078%);维生素C的含量以博斯克最高(9.50 mg/100g),绿宝石最低(1.89mg/100g);不同品种间可溶性蛋白和氨基酸的含量差异较大,其含量范围分别为127~428mg/100g和77.4~24.8 mg/100g;不同品种间同一矿质元素的含量存在较大差异。

Three primer pairs located in psbA~trnK, psbB~psbH and trnR-ACG~trnN-GUU regions were applied in phylogenetic analysis according to Maximum parsimony and Neighbor-joining methods. The consistent results between the phylogenetic analysis and taxonomy indicated the application potential of the three primer pairs in phylogenetic analysis and species identification. However, due to no sequence variation observed in amplified regions between two vegetable soybean varieties (KS5 and KS8), two cowpea subspecies (Vigna unguiculata ssp. cylindrica and Vigna unguiculata ssp. sesquipedalis), and among three rice varieties (TNG67, TK9, and TCS10), the three primer pairs are not effective in identifying variation within species.

选择其中三组引子对(基因体位置为psbA~trnK、psbB~psbH及trnR-ACG~trnN-GUU)增幅片段之序列进行亲缘分析,以最大简约法及邻接法之分群结果皆与目前植物分类相符,故三组引子对在物种间亲缘关系分析及物种鉴定上都有不错的效果,位於psbA~trnK及psbB~psbH之引子对效果尤佳,但是三组引子对在物种内变异的辨识效果不甚理想,在两个毛豆品种间(高雄5号与高雄8号)、两个豇豆亚种间及三个水稻品种间(台农67号、台稉9号与台中秈10号)的增幅片段没有明显差异存在。

The mechanisms for the differences among rice cultivars in Cd uptake and translocation There were significant differences in root oxidating abilities and soil redox potential between two typical rice cultivars, Shan you 63 and Wu yun jing 7, that are greatly different in Cd uptake from soil. The root oxidating abilities and soil Eh were accordant with Cd uptake for the cultivars. The difference between the two cultivars in Fe concentrations of root iron plaque was also significant. The cultivar with higher Fe concentrations in root iron plaque absorbed less Cd from soil.

四、水稻不同品种对镉吸收及分配存在差异的生理机理对土壤中Cd吸收能力差异很大的水稻不同品种间,根系氧化力和土壤氧化还原电位有显著差异,品种的根系氧化力和土壤氧化还原电位与该品种对Cd的吸收能力间有很好的一致性;水稻不同品种间,根表铁氧化膜的含铁量有显著差异,根表铁氧化膜含铁量高的品种,对土壤中Cd的吸收能力低;盆栽水分腾发量高及根系伤流液Cd浓度高的品种,吸收积累Cd的能力强。

The results showed that net photosynthetic rate P(subscript n, transpiration rate T(subscript r, stomatal conductance G(subscript s, intracellular concentration of CO2 C(subscript i, water use efficiency of cultivars and the light saturation point in leaves of 5 cultivars were different and had obvious differences, and the light compensation point was similar. Diurnal change of net photosynthetic rate among cultivars were different, three cultivars showed twin-peaked curve with an obvious midday-depress, others showed single peak curves.

结果表明:不同品种的净光合速率P(下标 n、蒸腾速率T(下标 r、气孔导度G(下标 s、胞间CO2浓度C(下标 i、水分利用率和光饱和点之间的差异明显,各品种的光补偿点均较低;净光合速率的日变化也存在明显的品种间差异,有3个品种呈&中午降低型&的双峰曲线,2个品种呈单峰曲线。

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Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严,以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆,把帆卷起,系住。

Therefore, positively advances the interest rate marketability reform is one of current our country finance reform important tasks.

因此,积极推进利率市场化改革是当前我国金融改革的重要任务之一。