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分裂

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The samples were smeared on slides and stained with Giemsas stain. The stained smears were observed microscopically and photographed. It showed that except typical trophozoites in binary fission. the following trophozoites of abnormal morphs were also observed. for instance abnormal trophozoites with binary fission; enlarged trophozoites with round and irregular shaped eosinophilic bodies in the cell plasma; enlarged cells containing round and irregular shaped eosinophilic bodies and flagella; enlarged cells which contain 6 or 8 nuclear-like bodies and flagella, 3 or 4 embryonic forms of daughter trophozoite in one mother trophozoite; 4 trophozoite embryonic forms with fused plasma 3 trophozoites with fused plasma;4 trophozoites with fused plasma; a pair of trophozoite with binary fission fused with another trophozoite;2 pairs of trophozoite with binary fission fused each other; a trophozoite with 1 nuclear only.

结果 除观察到典型的营二分裂法繁殖的贾第虫滋养体外,还可见到多种形态异常的虫体,包括;虫体呈非典型二分裂;滋养体胀大,胞质中有团块状和不规则形状的嗜酸性物质;在胀大的贾第虫细胞内含有团块状和不规则形状的嗜酸性物质以及鞭毛;在胀大的贾第虫细胞内含有6或8个核状物以及鞭毛;在一个母体细胞中含有3或4个子体细胞的雏形;胞质互相融合的4个滋养体的雏形;胞质互相触合的3个滋养体,胞质互相融合的4个滋养体;1对营二分裂的滋养体与另一个滋养休互相融合;2对营二分裂的滋养体互相融合;仅有1个细胞核的滋养体。

By the cooperation of 8-MOP/UVA, the photoaging-characteristic biological markers of the dermic fibroblasts in vivo and in intro were changed as follows:① with a permanent switch of mitotic to stably postmitotic phentypes, fibroblasts displayed growth suppression and morphological changes of cell senescence;② increasing expression of SA-β-galactosidase;③ increasing expression of p16 protein;④ continuous up-regulation of mRNA of MMP-1 and MMP-3, while the protein of TIMP-1 was only slightly induced;⑤ high levels of the 4977bp deleted mtDNA accumulated in dermis and cultured fibroblasts, and large accumulation of a A→C base transversion of 414 position of 〓 of human mtDNA control region for replication of cultured fibroblasts also.

2.8-MOP/UVA作用下,体内外真皮成纤维细胞生物学特性出现具有光老化特征性的改变:①细胞由具有分裂活性的分裂表型转化为不具有分裂能力的分裂后表型,形态学出现细胞衰老的相应改变;②SA-β-Gal表达增加;③p16蛋白表达增加;④MMP-1、MMP-3 mRNA持续表达而TIMP蛋白表达仅被轻微诱导;⑤mtDNA 4977bp缺失大量累积,培养成纤维细胞mtDNA复制控制区〓片段414碱基迅速出现大量A→C的点突变。

General characteristics of cells, its chemical constituents ( protein, nucleic acid, carbohydrate, lipid), cell structure , cell cycle, cell division, four kinds of tissues (epithelial, connective, muscular, and nervous), organ, ten organ systems (dermal, skeletal, muscular, digestive, respiratory, circulatory, excretory, endosecretory, nervous, and reproductive).

细胞的一般特征,细胞的化学组成(蛋白质,核酸,糖类,脂类),细胞的结构,细胞周期,细胞分裂(无丝分裂,有丝分裂,减数分裂),四大组织(上皮组织,结缔组织,肌肉组织,神经组织),器官,十大系统(皮肤系统、骨骼系统、肌肉系统、消化系统、呼吸系统、循环系统、排泄系统、内分泌系统、神经系统和生殖系统)。

It was showed that throughout the nuclear division, nuclear membrane did not disintegrate,lamina always existed; But only one lamin peptide (similar to lamin B in higher eukaryotes) was expressed during division,ant its expression quantity changed according to the progress of division: in interphase, lamin was constant; when entering G1 phase, lamin increased sharply; then, increased slowly untill division finished.

结果表明:在整个核分裂过程中,核膜不崩解,核纤层结构一直存在;但随着分裂的进展,始终只有一种成分(类似于高等生物lamin B )的表达;其表达量随着分裂时相的推移而变化:间期时,比较恒定,进入G1期时明显增加;以后不断略有增加,直到分裂完成。

In conclusion, these results suggested that (1) the ageing-associated decline in fertility of female KM mouse was due to a decrease in both quantity and quality of oocytes;(2) the ooplasm from young mice did not correct the meiotic errors of aged mice, and the ooplasm from aged mice did not induce abnormal segregation of meiotic chromosome of young mice, indicating that meiotic anomalies found in the oocytes of aged mice might be related to nucleus or chromosomes and relevant factors;(3) a critical nucleocytoplasmic ratio was essential for normal maturation and segregation of meiotic chromosomes of oocytes and development to 2-cell embryos.

本研究结果表明:(1)昆明白小鼠与衰老相关的生育力下降是其卵母细胞数量减少和质量下降的综合结果;(2)年轻小鼠卵母细胞细胞质不能纠正老龄小鼠GV的减数分裂错误,老龄小鼠卵母细胞细胞质也不诱导年轻小鼠的GV发生减数分裂错误,老龄小鼠卵母细胞减数分裂异常很可能与细胞核或染色体及其相关因素有关;(3)关键的核质比对卵母细胞正常的成熟、减数分裂染色体分离及2-细胞期胚的发育是绝对必需的。

The testis index, testis volumes were same as the annual changes of testis mass. The curves of annual variation were all unimodality.2 The spermatogenetic cycle of Myospalax cansus comprises seven stages with significant features: Stage I , from February to April, the testis were at the stage of spermatogonia proliferation. In this period, testis index and the number of spermatogonia began to rise. Other spermatogenic cells had not yet formed; Stage II to III, from March to April, primary spermatocyte meiosis period. The testis index was highest in this stage, and spermatogenic cells were in spermatocyte stage, the primary spermatocyte meiosis generated to secondary spermatocyte; Stage IV, from April to May, spermatocytes continued to split, germ cells appeared in seminiferous tubules; Stage V, in May, sperm formation, spermatids of seminiferous tubules were transformed to spermatozoa, a large number of sperms existed in the lumen; Stage VI, spermatozoa emission period, from May to June, testis index were a significant drop and mature spermatozoa excluded gradually; VII, the testicular activity ceased basically from July to September, November to January of the following year, the spermatogenic activity ceased completely. Therefore, Myospalax cansus are animals of seasonal reproduction, spermatogenesis cycle is discontinuous type.

睾丸系数、体积和重量的年周期变化规律一致,变化曲线呈现单峰型。2甘肃鼢鼠雄性生殖腺的年周期活动由7个特征明显的时期构成:Ⅰ期,2~3月份,精原细胞增殖期,睾丸系数开始上升,精原细胞进行有丝分裂,其他生精细胞尚未形成;3~4月份为Ⅱ~Ⅲ期,初级精母细胞成熟分裂期,睾丸系数达到最大,生精细胞大多处于精母细胞阶段,初级精母细胞减数分裂生成次级精母细胞;Ⅳ期,4~5月份精母细胞继续进行分裂,精细胞在生精小管内出现;Ⅴ期,5月份,精子形成期,曲细精管中精细胞变态成精子,在管腔中存在大量的精子;Ⅵ期,精子排放期,5~6月份,睾丸系数显著下降,成熟精子从生精小管上脱离,逐渐排除;Ⅶ期,精原细胞停滞期,7~9月份睾丸生精活动基本停滞,11~翌年1月,生精活动完全停止。

The first split of microspore mother cell is observed after one week after beginning of temperature arised under high temperature but the split of secondary sporogenous cell doesn't be found. The split of secondary sporogenous cell is still found after one week but the first split of microspore mother cell is observed after 12d under low temperature. Formating speed of male gametophyte is affected by temperature.

在高温条件下,开始加温1周后即看到小孢子母细胞进行第一次分裂,未观察到次生造孢细胞的分裂;在低温条件下,1周后仍能看到次生造孢细胞的分裂,12d后才看到小孢子母细胞进行第一次分裂

The events occurring during these stages differ in meiosis and mitosis, notably in that bivalents (pairs of homologous chromosomes) are formed in meiosis, whereas homologous chromosomes remain separate in mitosis.

在减数分裂和有丝分裂中,前期发生的事件是不同的,尤其是二价染色体区别最为明显,在减数分裂中形成,而在有丝分裂中同源染色体是分离的。

In prophase, it localized on the microtubule organizing-center. In metaphase, it co-localized with α-tublin completely on spindle. In anaphase, it co-localized with α-tublin in aster and mid-zone. In telophase, it co-localized with α-tublin in cytoplasmic bridge connecting the two daughter cells.

分裂前期,hSMP-1蛋白位于微管组织中心区域;在分裂中期,hSMP-1蛋白与组成纺锤体的微管呈完全共定位;在分裂后期,hSMP-1蛋白与星体和纺锤体中区的微管呈共定位关系;在分裂末期,hSMP-1蛋白则与两个子细胞之间的胞质桥完全共定位。

The meiosis of oocytes differ from mitoses of general cells. Especially in most mammalian species, oocytes are formed during fetal life, but they are arrested at the prophase stage of the first meiotic division until around the time of ovulation.

卵母细胞的成熟分裂有别于一般细胞的有丝分裂,尤其是哺乳动物,在胎儿期或出生后不久,初级卵母细胞就陆续进入第一次成熟分裂前期阶段,但长时间的&休止&于此期,直到性成熟时在促性腺激素等因素的作用下,第一次成熟分裂才重新启动。

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