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However, it is easy to arouse dispute and dissension in practical operation.

摘 要:分批分期装运条款是一种常见而又重要的装运条款,在实际操作中容易引起争议和纠纷。

Production time is shorter, dry weight of cell, MTG activity and production intensity increased by 16 %,27 % and 46 %,respectively,compared with that of MTG ...

MTG分批发酵与摇瓶相比较,发酵周期缩短了8hr,菌体细胞干重、酶活和生产强度分别增加了16%、27%和46%。

And by batch cultivation in fermenter, the optimal dissolved oxygen in fermentation solution was found to be 40%.

通过发酵罐中重组菌的分批培养,确定了培养液中的最佳溶氧量为40%。

Based on the above experiment, fed batch fermentation in 7-liter fermentor was performed.

在此基础上进行了7L发酵罐补料分批发酵试验。

Than that of fermentation in shake flask fed batch and 5-liter fermentor batch.

在此基础上进行了5L发酵罐补料分批发酵试验。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2~0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%~80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0~0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法,将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合,建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺:胰酶用pH4.5醋酸缓冲溶液提取后,粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5,洗脱缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5)含0.2~0.35mol/LNaCl分两步洗脱,收集激肽释放酶的洗脱峰;DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液:0.01mol/LTris-HCl缓冲液(pH7.5,洗脱液:0.5mol/LNaCl溶液,一次洗脱,收集胰蛋白酶洗脱峰;最后,亲和层析未吸附液用30%~80%硫酸铵分级盐析处理,沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5含1mol/L(NH_4)_2SO_4,洗脱缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5)含0~0.6mol/L(NH_4)_2SO_4,分两步洗脱,收集糜蛋白酶的洗脱峰。

Under certain acid and temperature conditions, urea added in batches to the phased reaction, a stable gluey liquid high strength adhesive f...

在一定酸度和温度条件下,分批加入尿素进行阶段反应,得到一种胶液性能稳定、黏结强度高的建筑板材用黏合剂。

Under certain acid and temperature conditions, urea added in batches to the phased reaction, a stable gluey liquid high strength adhesive for planks of building is produced.

在一定酸度和温度条件下,分批加入尿素进行阶段反应,得到一种胶液性能稳定、黏结强度高的建筑板材用黏合剂。

In the COST flow of a job order COSTing system, the account of Goods in Process is the heart.

分批法中,在产品账户为其核心。

Finally the high expression and low degradation of hirudin were achieved.

在分离纯化并鉴定出水蛭素的四种活性异构体后,通过恒化培养的研究探索影响水蛭素降解的因素,最终在分批发酵中实现水蛭素的低降解高表达。

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He and Nina moved to California and lived at 2005 Ivar Street, Apt.

他和Nina搬到加州,并在2005年伊瓦尔街,公寓生活。

Droperidol ( potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道,可能是 QT 间期延长的机制。

The Nazi's cruel treatment of the Jews during the Second World War is beneath contempt .

二战期间纳粹对犹太人的暴行极其可鄙。