再证实

Would you please go to the Booking Office to reconfirm your reservation.

请您到售票处去再证实一下您的预定票。

The molecular weight dependence of the distribfition of polymer molecules between the coacervate and the dilute phases on the phase separation of polymethylmethacrylate-acetone-methanol system has been proved to follow the relation first derived by Flory for binary systems: f"_j/f′_j = Qe~_j where f"_j and f′_j are the weight fractions of the polymer molecules of molecular weight M_j in the coacervate and in the dilute phases respectively, while Q and σ are parameters.

1。聚甲基丙烯酸甲酯-丙酮-甲醇体系的两次分相实验用θ-溶剂中的沉降速度法测定分相前试样和浓相的分子量分布曲线，得到高分子在浓稀相重量分配的分子量依赖关系 f_j=Qe~证实Flory的溶度函数形式与实际相符，并适用于三元体系的相分离，但Q值与两相体积比R值并不相同。 2。再分级使级分的分子量分布宽度随再分级次数的增加而减小，通常认为到第三次再分级其效果已不显著并不确实。

Requires reconfirmation of part quality in 1 month, and reliability in 2 months after start of production.

需要在1个月内再证实零件质量，并在开始生产的2个月内确认可靠性。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

The present study demonstrates that caffeic acid phenethyl ester, a major component of honeybee propolis, enhanced ATRA-induced granulocytic differentiation in HL-60, a human promyelocytic cell line. The differentiation was assessed by Wright-Giemsa stain, nitroblue tetrazolium reduction, and membrane differentiation marker CD11b.

经由实验结果发现，利用化学染剂(Wright-Giemsa stain)观察型态， CAPE也会促进低剂量 ATRA (1nM)分化的效用，而此种分化经由 NBT reduction，和透过流式细胞仪分析分化标的膜蛋白 CD11b/CD14及细胞周期测试后，证实细胞周期停滞 G1 phase与分化现象的机制有关；再藉由免疫沉降法测试，更证实细胞周期停滞将影响 CDK2的活性。

Subsequently, a l kb fragment of HCCR-2 encoding region was amplified by RT-PCR and cloned into Promega TEasy vector.

测序证实无误后，以HCCR-2／T载体为模板，再通过PCR克隆出HCCR-2的编码区序列，将其连接到pIRES2-EGFP真核表达载体，并通过测序获得证实。

The second part of the thesis consists of three chapters, which focus on arguing for the rationality of "the unverifiable value judgment" from three points, the verification on the value judgment and emotion, the verification on value judgment and the language logic and the verification on the value judgment and rationality. The fourth chapter mainly discuss the verification on value judgment and emotion.

本文第二部分，即第四、五、六这三章，集中反驳&价值判断是不可证实的&这一论断的合理性，主要是从逻辑实证主义和情感主义对价值判断可证实性的三方面攻击入手，分别对情感与价值判断的可证实性、语言逻辑与价值判断的可证实性和理性与价值判断的可证实性三者进行再考察，第四章主要探讨情感与价值判断可证实性。

Expression of pRb in HIC/S cell line was proved again by immunohistochemistry and semi- quantitated with Western blot analysis.

应用S-P免疫组化技术对已报道的HIC/S细胞的Rb蛋白表达状态进行再证实，并采用Westernblot法对HIC/S细胞的Rb蛋白作半定量分析。

D Study and reconfirm your assessment of the company, then smile and buy more?

研究并再证实那个公司的评估，然后笑笑买更多的该公司的股票？

Stay in the stopping place more than 72 hours, otherwise their reservations will be automatically cancelled.

座位再证实旅客持有定妥的联程或来回程客票，如在该联程或回程地点停留72小时以上，须在该联程或回程航班飞机离

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。