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Methods In this study,a plasmid vector,rAd5,and rAAV2 were used to transfect rat cochlear marginal cells of stria vascularis by injection into the perilymph through the round window membrane,and the transfection efficiency, target tissue accessibility, cell/ tissue toxicity, time course of expression, and effect on hearing were evaluated in vivo and in vitro.

结果三种载体对原代培养的大鼠耳边缘细胞的转染研究发现,腺病毒的转染效率最高,而腺相关病毒转染对细胞活性影响最小。rAAV2和rAd5对大鼠耳蜗组织的感染实验发现,腺病毒和腺相关病毒携带的增强型绿色荧光蛋白可在多种耳蜗组织中及转染对侧耳蜗组织表达,且并不引起明显的耳蜗组织细胞凋亡。rAAV2携带的EGFP基因在第90天时仍可检测到大量表达。rAd5携带的EGFP基因在第30天时表达明显减弱。

And they obeyed not, nor inclined their ear: but walked every one in the perverseness of his own wicked heart: and I brought upon them all the words of this covenant, which I commanded them to do, but they did them not.

他们不但不侧耳听从,反而各随自己顽固的恶心生活;为此,我对他们执行了我命他们遵行,而他们没有遵行的这盟约上的一切话。

Listen to him, and you will hear the loudness, the quality and the musical note of his voice always changing according to what he is talking about.

侧耳倾听,你会发现他声音的轻重、嗓音的特质以及音乐般的语调总是随着所讲内容的改变而改变。

The aimwas to explore the influence of Sargassum extract liquid on Pleurotus ostreatus growth so as to provide basis for its exploitationand utilization.

探究马尾藻提取液对侧耳生长的影响,为其开发利用提供依据。

Give ear, O God, to my prayer; And do not hide Yourself from my supplication.

55:1 神阿,求你侧耳听我的祷告;不要隐藏不听我的恳求。

The Digital Multimedia and interactive learning function will make you feel more relaxed and interested in learning Yuga.

直筒状造型附加两侧耳音源输出,影音效果互动式的学习,让瑜珈练习更加轻松有趣,实现未来无限可能。

Nineteen China cultivated strains of Pleurotus ostreatus were antagonistically paired on poor nutrient half-PDA plates. The rate of barrage formation was 100% among these strains.

对收集的糙皮侧耳Pleurotus ostreatus 19个栽培菌株在贫营养的半PDA培养基中配对进行拮抗线实验,考察其相互间体细胞不亲和性。

The control effects and application methods of Pleurotus ostreatus on the peanut root knot nematode disease, Meloidogyne arenaria, were systematically studied in the greenhouse.

通过盆栽试验研究了杀线虫担子菌-粗皮侧耳对花生根结线虫病的防治作用及施用技术。

Pleurotus ostreatus ; Meloidgyne arenaria ; Peanut root knot nematode disease ; Biological control

粗皮侧耳;根结线虫;花生根结线虫病;生物防治

Now mine eyes shall be open, and mine ears attent unto the prayer that is made in this place.

7:15 我必睁眼看,侧耳听在此处所献的祷告。

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Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严,以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆,把帆卷起,系住。

Therefore, positively advances the interest rate marketability reform is one of current our country finance reform important tasks.

因此,积极推进利率市场化改革是当前我国金融改革的重要任务之一。