体外发育

Mechanical environment, as a native survival one, is very complicated and constituted of many different kinds of forces, such as the forces in vitro or between tissues and cells, and the interactive intracellular stress during the process of formation and remodification of cytoskeletons. All these forces regulate bodies' developments and cellular differentiations, which is particularly obvious in bone tissues.

力学环境是自然赋予生命体的一种生存环境，在这个复杂的环境中，生物体外的力、生命运动过程中产生的体内各组织、细胞间的相互作用力及细胞骨架形成和变化时产生的细胞内张力交互作用，构成了一个力学系统，调节着生物体的生长发育和细胞分化，这一点表现在力对骨骼系统的作用上尤为明显。

The present study was performed to investigated the effects of sex hormones on the cell proliferation and differentiation of embryonic chick pituitary cells and peripheral neurons in vivo and in vitro, we injected specific corresponding receptor inhibitor in vivo to investigate the related mechanisms of sex hormones on the peripheral neurons.

本研究通过体内实验、体外实验研究了性激素对鸡胚垂体细胞和鸡胚外周神经元的增殖、分化的调节功能，通过在体注射性激素受体的特异抑制剂来研究性激素对外周神经元作用的相关机理，并通过检测鸡胚和绵羊胚胎垂体发育过程中雌激素受体的变化对雌激素调节内分泌细胞的作用机理进行了比较研究。

Embryonic stem cells derived from inner cell mass of mammalian blastocyst-stage emyst-stage embryos and primordial germ cells are totipotent cells that can be cultured in virto.

胚胎干细胞是从哺乳类动物的囊胚内细胞群或原始生殖嵴细胞分离、体外培养获得的具有发育全能性的细胞。

Effect of ethanol on the development of mouse visceral yolk sac.

乙醇对小鼠胚胎脏层卵黄囊发育影响的体外研究。

Three types of cells were transferred into perivitelline space under zona pellucida of rabbit oocytes respectively.

用体外培养14代和24代的小鼠胚胎干细胞构建的异种重构卵的卵裂率分别是88.5％和82.1％(P＞0.05)，囊胚发育率分别是16.7％和15.4％(P＞0.05)。

RESULTS Human ovarian follicles became steroidogenically active, and developed from the early secondary to antral stage in vitro.

结果人卵巢卵泡在体外培养30天后变成了具有生成类固醇激素的活性卵泡，并从早期次级卵泡发育成窦状卵泡。

Methods: Selected 93 confirmed cases with hypospadias during May 2003 to Sep 2009. Patients in balanic type were treated with meatal advancement and glandularp lasty; patients with urethra orifice at 2/3 of corpus penis accompanied with slight chordee of penis were treated with Snodgrass method or Onlay method; patients with severe chordee of penis were treated with Duckett method; patients in scrotum or perineum type were treated with combination of Duplay and Duckett; severe hypospadia patients were treated with curved pedicle penis-scrotum and skin flap urethroplasty; patients with good outer plate of ventral prepuce and chordee of penis were treated with ventral pedicle hooded foreskin flap.

选取2003年5月～2008年9月期间收治的93例确诊尿道下裂患者，对阴茎头型患者采用尿道口前移、阴茎头成形法；尿道口在阴茎体外2/3段伴有阴茎轻度下弯者采用尿道板纵切卷管法或加盖岛状皮板法（Snodgrass术或On-lay术）；有严重阴茎下弯的所有阴茎体型采用横裁或纵裁包皮岛状皮瓣尿道成形；阴囊型或会阴型用阴囊中缝皮管加横裁包皮岛状皮瓣成形尿道（Duplay联合Duckett术）；对重度尿道下裂患儿采用弧形带蒂阴茎阴囊联合皮瓣尿道成形术；对腹侧包皮外板发育良好患儿及合并阴茎下曲的患儿采用腹侧带蒂帽状包皮皮瓣法。

Methods: Selected 93 confirmed cases with hypospadias during May 2003 to Sep 2009. Patients in balanic type were treated with meatal advancement and glandularp lasty; patients with urethra orifice at 2/3 of corpus penis accompanied with slight chordee of penis were treated with Snodgrass method or Onlay method; patients with severe chordee of penis were treated with Duckett method; patients in scrotum or perineum type were treated with combination of Duplay and Duckett; severe hypospadia patients were treated with curved pedicle penisscrotum and skin flap urethroplasty; patients with good outer plate of ventral prepuce and chordee of penis were treated with ventral pedicle hooded foreskin flap.

选取2003年5月～2008年9月期间收治的93例确诊尿道下裂患者，对阴茎头型患者采用尿道口前移、阴茎头成形法；尿道口在阴茎体外2/3段伴有阴茎轻度下弯者采用尿道板纵切卷管法或加盖岛状皮板法(Snodgrass术或Onlay术)；有严重阴茎下弯的所有阴茎体型采用横裁或纵裁包皮岛状皮瓣尿道成形；阴囊型或会阴型用阴囊中缝皮管加横裁包皮岛状皮瓣成形尿道(Duplay联合Duckett术)；对重度尿道下裂患儿采用弧形带蒂阴茎阴囊联合皮瓣尿道成形术；对腹侧包皮外板发育良好患儿及合并阴茎下曲的患儿采用腹侧带蒂帽状包皮皮瓣法。

By the method of in situ hybridization, the distribution of mRNA of RSG6 was detected. The result showed that the transcript activity began at the late unicellular stage microspore, increased at the bicellular pollen, and was most vigorous at the two sperm cells in mature pollen.

用地高辛RNA标记试剂盒，采用体外转录方法标记RSG6基因的cDNA序列中长度为258bp的一段作为反义RNA探针，运用RNA原位杂交技术，探讨了RSG6基因的mRNA在水稻根、茎、叶以及花药发育各阶段组织中的分布情况。

Part IIMorpholino-mediated translational inhibition and overexpression oftbx2 in zebrafish cardiogenesis Objective To elucidate the role of tbx2 in zebrafish cardiogenesis via morpholino-mediated translational inhibition and overexpression on the base of expression pattern of tbx2. Methods Gene-specific antisense morpholino oligonucleotides were designed to against ATG region of zebrafish tbx2 to knockdown endogenous tbx2 gene.

设计tbx2-MO和对照MO，在斑马鱼受精卵的单细胞期，进行不同浓度的显微注射，并通过荧光蛋白融合标记实验和tbx2-mRNA、tbx2-MO共注射拯救实验，验证tbx2-MO抑制tbx2基因表达的有效性和特异性；基因过表达是通过给斑马鱼受精卵注射体外合成的tbx2-mRNA，来研究tbx2基因功能获得对斑马鱼胚胎心脏发育的影响。

He and Nina moved to California and lived at 2005 Ivar Street, Apt.

他和Nina搬到加州，并在2005年伊瓦尔街，公寓生活。

Droperidol （ potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道，可能是 QT 间期延长的机制。