体外发育

According to the mechanism of block of development in vitro culture on early embryo of mammal and in vivo surroundings of early embryo, the paper states that requirement and utilization of nutrients during each cell stage of early embryo of mammal in vitro culture in order to search for in vitro culture condition and method to improve the development rate of blastosphere.

摘要从哺乳动物早期胚胎体外培养发育阻断机理和早期胚胎的体内环境入手，阐述了胚胎体外培养各细胞阶段胚胎对营养物质的需求，寻求合理的体外培养条件和方法，以便提高体外胚胎早期的囊胚发育率。

Objective To observe the development of mouse kidney in vitro at embryonic day 12、14、16 and postnatal day 1, and compare with the development of homeochronous kidney in vivo.

目的 观察胚龄12、14、16、以及生后1天的肾组织体外培养的发育情况，与同期在体发育进行比较，探讨肾组织体外培养的胚龄选择。

By this the system, bovine preantral follicles in different diameters were cultured in vitro;and the effects of the number of follicles in each well of culture plate, different concentrations of NGF, Vitamin E, and Vitamin C on in vitro development of preantral follicles were studied.

在96孔培养板中，每孔25μl培养液的条件下，利用此系统研究了卵泡的入培直径、共培养卵泡数及神经生长因子、维生素C和维生素E对腔前卵泡体外发育的影响。

Objective To observe the effects of coculture of mouse embryo with endometrial epithelial cells on early embryonic development in vitro.

目的：采用子宫内膜上皮细胞与小鼠胚胎共培养，观察其对早期胚胎体外发育的影响。

Methods Mouse endometrial epithelial cells were isolated and cultured,then it was cocultured with mouse embryos fertilized in vitro.

目的：采用子宫内膜上皮细胞与小鼠胚胎共培养，观察其对早期胚胎体外发育的影响。

In order to study the effect of bovine fetal SCs feeder layer on the development of bovine zygotes in vitro.

研究牛胎儿睾丸支持细胞对牛早期胚胎的体外发育是否有促进作用。

In addition, we studied the distribution of mitochondria in metaphase Ⅱ rabbit oocytes, zygotes and early embryos by two active mitochondria dyes and revealed the change of active mitochondria during early development of rabbit embryos. We also found that the distribution of microfilaments and microtubules have similar patterns to that of active mitochondria, which is an important basis for further development of animal cloning study using the recipient oocytes derived from rabbit.

此外，我们应用两种线粒体荧光染料研究了日本大耳白兔体内成熟卵母细胞、正常交配后收集的受精卵及早期体外发育胚胎的活性线粒体的位置变化，首次揭示了兔早期胚胎中活性线粒体的变化特征及其与微丝微管变化的相关性，为进一步开展以兔卵母细胞作为核移植受体的动物克隆研究打下了重要的基础。

Methods Totally 630 zygotes were collected from the oviducts of superovulated female Kunming mice, which were cultured in protein-free potassium simplex optimized medium supplemented with Eagle's essential amino acids and Eagle's non-essential amino acids of different concentrations (mKSOM, mKSOM+1/16AA, mKSOM+1/8AA, mKSOM+1/4AA, mKSOM+1/2AA, mKSOM+AA, and mKSOM+2AA).

方法从超排昆明小鼠输卵管中取出的630枚受精卵均分为7组，分别在经添加不同浓度Eagle's氨基酸的去牛血清白蛋白KSOM培养液(mKSOM、mKSOM+1/16AA、mKSOM+1/8AA、mKSOM+1/4AA、mKSOM+1/2AA、mKSOM+ AA、mKSOM+2AA)中培养，对比观察各组胚胎体外发育的过程。

Sequential culture system is more suitable for the development of cultured human oocytes and embryos.

结论采用适合的培养液进行序贯培养，更适合于卵子及胚胎的体外发育，方法安全可行。

Studies on mouse nuclear transfer with adult male mouse ear fibroblast cells as donors In this study, C57BL/6 adult male mouse ear fibroblast cells and Kunming mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different passages of donor cells and electrofusion times on the in vitro development of NT reconstructed embryos, when the oocytes were treated with 3% sucrose.

以成年雄鼠耳成纤维细胞为供体克隆小鼠的研究该研究以C57BL/6 成年雄鼠耳成纤维细胞为供体，昆明白小鼠MⅡ期卵母细胞为受体，研究了3%蔗糖处理卵母细胞去核后，供体细胞不同传代次数和电融合次数对核移植重构胚体外发育的影响。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。