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It is indicated that loosely compacted soil is of strain-softening behaviour under undrained conditions, accompanied with a rapid increase in excess pore water pressure. In anisotropically consolidated constant shear stress tests, a very small axial strain was required to induce the failure and the excess pore water pressure increased quickly at failure.

研究表明：松散填土在不排水条件下具有应变软化的特性，软化过程中伴随着孔隙水压力的迅速上升，有较高的液化势；在常剪应力排水剪条件下，具有破坏前轴向应变小、破坏时间短，破坏过程中孔隙水压力迅速上升。

However, the area is less studied than other areas of endophyteresearch world wide. Therefore, a series of experiments were carried out in laboratory,glasshouse and field to compare the responses of endophyte infected plants andendophyte free plants of perennial ryegrass varieties to 10 fungal pathogensisolated from turf type ryegrass in Lanzhou. The pathogens used in the experimentswere Alternaria alternata、Bipolaris sorokiniana、Fusarium avenaceum、Curvularialunata、Ascochyta leptospora、Fusarium acuminatum、Fusarium chlamydosporum、Fusarium oxysporum、Fusarium solani and Gliocladium roseum. In addition, someprotective enzymes activity and alkaloids contents of E+ and E- plants were measuredand LpsA gene of the common strain - Lp19 and mutant strain -AR1 of Neotyphodiumlolii were sequenced to identify their ...

本研究在初步明确兰州市坪用黑麦草内生真菌及其病原真菌多样性的基础上，在实验室、温室和田间开展了系列研究，比较了带内生真菌和不带内生真菌的黑麦草在离体和整株条件下对细交链孢、离蠕孢、燕麦镰孢、新月弯孢、小孢壳二孢、锐顶镰孢、厚垣镰孢、尖镰孢、腐皮镰孢和粉红粘帚霉等10种病原真菌的抗病性，测定了E+和E-植株数种保护酶活性及其生物碱含量的变化。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Biological characteristics of the avirulent strain 061, 61 - 2 and 61 - 3 isolated from the roots of glycine max inoculated with the avirulent strain 061 were studied.

对鼠疫弱毒株061及用该株菌接种大豆后不同时期从大豆根中分离的61－2、61－3株的某些生物学特性进行比较研究。

The inhibition rate of ANTI-8098A to the virulent strain was 0.158, 40.6% higher than that to the avirulent strain.

强致病力菌株达到0.158的抑制增长率，比无致病力菌株高40.6％。

Suppression subtractive hybridization was used between virulent strain HA9801 and avirulent strain 12. Five fragments of putative virulent genes were found by this method.

并采用抑制性差减杂交的方法对2型毒力菌株所携带的差异基因序列进行了研究，发现了5个新的可能的毒力基因。

Employing capillary-pipette washing method,the unialgal strain and axenic strain of Microcystis aeruginosa from Songhua Lake were isolated and purified,and effects of temperature,illuminance,nitrogen,phosphorus,and iron on their growth were investigated.

用毛细滴管洗净法从松花湖分离纯化出铜绿微囊藻的单藻株和无菌株，研究了温度、照度、N、P、Fe各种因素对单藻株和无菌株生长的影响。

The km mouse had lower white blood\|cell count than that of BALB/c and C 57 BL/6J inbred strain;The BALB/c inbred strain had lower eosinophil and basophilic cells tha...

小鼠具有很多的品种品系，是实验动物中培育种类、品系最多的动物，也是医学研究领域应用最广泛的动物。因此

A bacterial strain using phenol, benzoate, naphthalene, biphenyl and benzothiophene as sole carbon and energy sources was isolated from waste sludge and was identified as Rhodococcus sp. strain DF51 according to morphological, physiological and its 16S rRNA gene sequence.

从太原市郊苯酚污染的土样中分离到一株能以苯酚、苯甲酸、萘、联苯和苯并噻吩为唯一碳源和能源生长，并且具有同时分解单环和双环芳香类物质能力的菌株，经生理生化和16S rDNA基因序列分析鉴定为红球菌DF51Rhodococcus sp。

In this paper, an efficient and easy biological screening method based on the sensitivity to lovastatin of Neurospora crassa was built to deal with the mutant strain rudely by UV mutation. A high-level strain was screened in the shaking flask culture incorporating with TLC and HPLC. A comparison between this two methods also was originally discussed while identifying dyeing using bromophenol blue as the better treatment of TLC, and offering a kind of simple, practical, fast and economical determination qualitatively and quantitatively.

本文有鉴于此，通过紫外诱变处理，并基于粗糙脉孢酶对洛伐他汀的生物敏感性，对诱变菌株进行了初筛；同时，采用摇瓶培养，结合TLC和HPLC的分析方法，复筛出一株高产菌株；对复筛过程两种分析方法分别进行了初步探讨，比较其优劣，确定TLC法经溴酚兰显色剂显色处理为较佳方式；从而，建立了一套简单、实用、快速、经济且有效的洛伐他汀生产菌诱变筛选方法。

We are forbidden from giving money to these shoeless, skinny children, as this will perpetuate begging.

向光着脚，骨瘦如柴的孩子施舍是不可以的，因为要招来无休无止的乞讨。

It would be hard not to fall victim to some sharpeyed policeman.

要想不被某个目光锐利的警察抓到，谈何容易。