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The invention relates to a new strain producing taxol and a taxol preparation method. The taxol is a diterpenoid alkaloid drug which is extracted from taxaceae plants and has a plurality of anti-tumor effects. Taxus, also known as yew, is a prehistoric plant with a history of 0.2 billion years and is a rare plant species with low growing speed; the biological characteristics of the taxus have high demand on the ecological environment; the natural renewal ability is poor, so the amount of the species is very limited, belonging to an endangered protection plant with limited resource, thus, scientists at home and abroad actively research and develop new taxol sources. The new strain for producing the taxol of the invention is the strain which is first separated from the bark of T.cuspidate Sieb.

产紫杉醇的新菌种及其选育和制备紫杉醇的方法，紫杉醇是从红豆杉属植物中提取出来的具有多种抗肿瘤疗效的二萜生物碱类药物，由于红豆杉，又名紫杉，是两亿年前的史前植物，是珍稀树种，生长速度缓慢，并且其生物学特性对生态环境要求较高，天然更新能力差，所以种群数量极为有限，属濒危保护植物，资源十分有限，因此，国内外学者都在积极地研究开发紫杉醇的新药源，本发明产紫杉醇的新菌种，所述的新菌种为从东北红豆杉T.cuspidate Sieb。

The susceptible strain, the abamectin resistant strain and the high temperature tolerant strain from Tetranychus cinnabarinus were used to study cross tolerance to abamectin and high temperature using the methods of bioassay and fluorescent real time quantitative PCR.

以朱砂叶螨敏感种群、抗阿维菌素种群和耐高温种群为材料，应用生物测定方法以及荧光实时定量PCR技术，研究朱砂叶螨抗药性和耐高温间存在的交互耐性及机制。

To determine the regulating role of mtlR in transcription of mannitol PTS operon in V.cholerae,and whether it plays a role in the ferment difference of the toxigenic and non-toxigenic strains,the mtlR deletion mutants from the mannitol rapid-ferment strain(non-toxigenic strain) and slow-ferment strain were constructed.Comparisons of the growth in M9 containing 0.2% mannitol as the sole carbon source and pH change in mannitol fermentation media of these wild strains and their mutants,indicated that mtlR is a repressor.Its repression in mtlCBA transcription was further verified with the analyses of quantitative reverse-transcriptional PCR.

通过比较快发酵菌株和慢发酵菌株mtlR缺失突变株与野生株在含0.2%甘露醇的M9培养液及甘露醇发酵液中生长、产酸等的变化，定性地证明了mtlR基因的抑制作用；另外通过定量RT-PCR进一步验证了MtlR蛋白在mtlCBA转录水平发挥负调控作用。

Analysis of these mutants revealed that the expression level of the neutral trehalase gene in RNAi mutant (Ma688 strain) was reduced to 42% of which found in wild-type strain and the over-expression mutant (Ma113 strain) showed approximatlely 2-fold higher levels of neutral trehalase mRNA than that found in wild-type control. Then the transformants Ma688 and Ma113 were chosen for closer examination, including trehalose content, storage longevity , thermotol

利用Northern blot 分析这几个突变菌株中性海藻糖酶基因mRNA 的含量，选择中性海藻糖酶基因超表达2 倍和中性海藻糖酶基因被抑制到42%的两个转化菌：Ma113 和Ma688，将其与出发菌株进行分生孢子内海藻糖含量的测定，孢子储藏

Contrasting with the control strain of Shigella sonnei, the test strain could be higher antibiotics rate, and it could not be agglutinated by several anti-sera to Shigella sonnei, which were in the period of validity, but the control strain could be well agglutinated.

检测试验菌株的生化特性和对抗生素的敏感性，以及用多种批号的诊断血清进行凝集试验。结果：具有典型的宋内氏志贺菌的生化特性；与对照菌相比，有较高抗生素耐药性，对复方新诺明等多种抗生素耐药。

Additionally, one SsDRV-free strain Ep-1PNA183av that showed hypovirulence was isolated from a mutant of healthy strain Ep-1PNA183, this strain also immunizes the infection of SsDRV, it would be a valuable material to study on the interaction between SsDRV and its host. Surprisingly, we discovered that SsDRV could be transmitted into S. nivalis (in the same genus to S. sclerotiorum) when cultured side by side on PDA plate, this discovery may supply new evidence for the exchange of genetical elements among different species in fungi; the transmission of mycovirus to other species showed that the limitation of vegetative imcompatiblity among different strains might not be key factor to determine the efficiency of biological control with hypovirulence associated mycovirus.

发现了1株不携带SsDRV的核盘菌弱毒力菌株，该菌株对SsDRV具有显著抗性，是研究SsDRV与寄主互作的宝贵材料；还发现SsDRV可以在核盘菌种间传递，深入研究将为生物在种间的遗传物质交换提供了新的证据，也将为广泛利用弱毒相关病毒控制植物病害提供理论依据。

High yield strain and low yield strain were tested and showed the biological characteristics diversity: the color of hydrolyzed grease in plate and determining the dissociated acid of fermentation fluid straightly relate to the producing of spinosyn, then establishment of the rapid screening pattern for spinosyn overproducing strain

研究产量差异大的高产菌株和低产菌株的各种生物学特性，发现通过油脂水解平板的颜色以及发酵液的酸价，与菌株的发酵单位有直接相关性，从而建立了快速筛选模型。

Passaging S79-1-3 strain in CEC cell cultures may result in selection of its substrain compositions, S79-1-3 strain was less stable than S79-1-P7-3③ strain.

结论S79株病毒与文献报道的JL2株十分相似，经连续传代总体的核苷酸差异〈0.5％。S79-1-3主代病毒在药典规定代次范围内甚为稳定。

To analyze effect of diclazuril and maduramycin on 18S rDNA of Eimeria tenella, the 18S rDNA of sporulated oocyst of the E. tenella anti-diclazuril strain, the E. tenellaanti-maduramycin strain and the E. tenella drug-sensitive strain were cloned and sequenced, and were compared by using DNAStar 5.0 and RNADraw 1.1, respectively.

为探讨抗球虫药物地克珠利和马杜拉霉素对柔嫩艾美球虫18S rDNA的影响，分别对人工诱导的柔嫩艾美球虫地克珠利抗药株、马杜拉霉素抗药株和药物敏感株孢子化卵囊的18S rDNA基因进行了克隆测序，并通过生物信息软件进行对比差异分析。

HIV-1B 'strain is endemic in Asia's three major HIV-1 strain of the blood collection in the central region of China's HIV infected persons in the broad popular. B 'strain with other subtypes of HIV-1 strains combine, hastened the birth of a variety of HIV-1 recombinant virus.

HIV-1B'毒株是亚洲流行的HIV-1三大主要毒株之一，在我国中部地区采供血HIV感染者中广泛流行。B'毒株与其他HIV-1亚型毒株结合，催生了多种HIV-1重组型病毒。

On closer examination, though, this is not a vote for multilateralism but just the opposite.

仔细审视后我们发现，这并非是对多边主义投出的赞成票，而是恰好相反。

Uncovering their weak spots, so I can defeat them.

揭露出他们的弱点，这样我就可以打败他们了。