查询词典 sequencing problem

The characteristics of operational performance and membrane fouling were investigated using synthetic wastewater as feed in a sidestream membrane sequencing batch reactor system.

采用分体式MSBR和人工合成配水，对处理系统在进料高负荷变化条件下的运行特性和膜污染表现特征进行了研究。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

DNA sequencing of positive clone was analyzed by Automated DNA sequencers,and their homologous sequences were searched in GeneBank.

经DNA 测序后，在Genebank 中与人的免疫球蛋白库进行比对，确定为单链抗体片段。

Moreover, a new archive for sequencing data from next-generation sequencers, the 'DDBJ Read Archive', was launched.

此外，来自于下一代测序仪的测序数据的一个新的存档----&DDBJ片段存档&----也被启动。

Genset , which hopes to have the drug in clinical trials by the end of 2001, started out developing techniques for gene sequencing and other basic research and wound up discovering several genes for prostates cancer.

该公司想把此药在2001年底投入临床试验。该公司开始开发基因测序和其他基础技术研究，并意外地发现了前列腺癌的几个基因。

Due to potential instability of the prophages carrying the stx gene, in this study, the prophage of O157:H7 86-24 strain was cured with serial passages of bacteria, confirmed by PCR and DNA sequencing. The cultural supernatant of O157 prophage curing mutant was inoculated into Vero cells, indicating that the prophage curing mutant of O157 lost the toxigenicity to Vero cells.

由于原噬菌体本身具有潜在的不稳定性，本研究以O157：H7 86-24为始发菌株，将其进行传代培养，筛选原噬菌体消除菌株，对原噬菌体在O157基因组中的整合位点进行分析，并用Vero细胞对原噬菌体消除菌株的细胞毒性进行鉴定。

The aim of our study was to seek the mutations in MODY1~5 genes in Chinese population by direct sequencing in probands from families with early-onset type 2 diabetes.

通过对早发2型糖尿病家系先证者进行直接测序，寻找中国人群中可能存在的青少年的成人发病型糖尿病1-5基因突变。

Given that we expect a long phase in which growth is past its worst but well below trend, it is important to think more carefully about the likely sequencing of risk premia and asset returns, and whether it is the trough in growth or in the output gap (or the labour market parallels of a peak in initial jobless claims versus the peak in the unemployment rate) that matters most for different kinds of assets.

鉴于我们期望一个长期的阶段，经济增长是过去最差的，但远低于趋势，重要的是要想更仔细的可能测序的风险溢价和资产回报，以及它是否是谷底增长或在产出缺口（或劳动力市场平行的高峰，首次申请失业救济人数兑高峰期的失业率）最重要的不同种类的资产。

Biochemical and functional analysis of poxvirus genomes, genes, and proteins has entered a new era with the recent sequencing of more than 30 poxvirus genomes.

随着最近超过30个痘病毒基因组的测序，痘病毒基因组，基因和蛋白质的生物化学和功能性分析已经进入了一个新的时代。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。