查询词典 sequencing problem

Environmental bacteria ; Genetic diversity ; 16S ribosomal RNA gene ; Gene tag serial sequencing

环境细菌；遗传多样性； 16S核糖体RNA基因；基因标签串测序法

Dendritic cells are the most potent antigen-presenting cells in human immune system. Our institute has been working on the region of DC development and DC-mediated immune therapy on cancers for a long time. A cDNA library of human dendritic cells has been set up and by large-scared random sequencing, we have isolated a lot of novel fulllength cDNA clones.

树突状细胞(Dendritic cells，DC)是人体内重要的抗原提呈细胞，本研究所对DC的分化发育及其介导的肿瘤免疫治疗的研究工作已进行多年，并在此基础上建立了人树突状细胞基因文库，通过大规模随机测序的方法开展了从DC中克隆新分子的工作，从2万余条EST中发现了许多新的分子。

Finally, the constructed β3AR gene antisense expression vector was identified though restriction endonuclease analysis and sequencing. RESULTS: Target gene of about 1.2 kb was successfully cloned into retroviral vector pLNCX. The sequence of cloned β3AR fulllength cDNA was identical to the reported one in GenBank.

结果：经琼脂糖凝胶鉴定，所克隆的目的基因大约为1.2 kb，并成功地连接到逆转录病毒载体pLNCX上，经测序证明，插入的目的片段其碱基序列与Genbank上报道的完全一致，且插入载体的方向完全正确。

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

Panels of neutralising antibodies are being developed for each serotype and characterised by nucleotide sequencing of escape mutant virus.

针对每一个血清型中和抗体反应板已经开发出来，加入并据核苷酸序列检定免疫逃脱型变异毒种。

Coli DH5α, the positive recombinants were identified with restriction enzyme analysis and DNA sequencing analysis.

将阳性重组子转入大肠杆菌DH5α，用IPTG诱导重组EBP融合蛋白的表达。

In the present study, we have ascertained two Chinese families with HHD, and examined ATP2C1 gene mutations in these two families by direct sequencing.

在此研究中，我们收集了两个中国汉族人HHD家系，并通过直接测序的方法对这两个家系成员进行了ATP2C1基因的突变检测。

The transfer of genes from one species to another especially between remote species was emphasized by the information from genome sequencing.The problems about evolution phylogenies and safety of GEMs for HGT were discussed.

并结合基因组序列分析，重点阐述远缘生物之间发生的广泛的基因交流，以及其与进化、系统发育和基因工程生物安全性问题的探讨。

The research region focus on the genomic sequencing, disease genomics, functional genomics, bioinformatics.

其主要研究领域是基因组测序，疾病基因组，功能基因组和生物信息学。

The selective rules and sequencing rules of syntagmatic relation are not same, too.

六、义位组合的选择规则和序列规则不同方言亦不尽相同。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。