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With primers designed according to sequence(AY052417) of diapause pupae of oriental tobacco budworm registered on GenBank, a double-strand cDNA fragment of DH gene was obtained from RT-PCR amplification and was further cloned into pGEM-T Easy vector.

根据GenBank发表序列(AY052417)设计引物，以烟夜蛾(Helicoverpa assulta Guenée)滞育蛹为材料，利用RT-PCR技术扩增到滞育激素(diapause hormone，DH)cDNA，并通过TA克隆的方法将其克隆至pGEM-T Easy载体上，对目的基因进行测序，并利用设计的酶切位点将目的基因从T载体上切下。

This invention discloses a multi-queue sequence buffer management circuit and a method based on a pipeline applying a pipeline structure including: an arbitration circuit selecting one for process from read, write and distribution buffer requests, a buffer slot state module designing state of the slot requiring operation and queue numbers and assigning idle slots, a buffer slot filter module filtering the slot, a buffer slot filter module filtering the slot states not belonging to the current operation queues nor idle aligned in terms of the head pointer, a queue slot selection module computing continuous idle slot numbers from the slot pointed by the head pointer and refreshing the head pointer and selecting preparing slots, a queue slot prior queuing module refreshing the read pointer and result numbers of the current operation queues with the pointer of the first prepare slots and their numbers which can support multi-queue to share one buffer space, queues can access the buffer in overlap.

本发明公开一种基于流水线的多队列顺序化缓冲管理电路及方法，本发明电路采用流水线结构，包括：仲裁电路，从读、写、分配缓冲请求中选取一路进行处理；缓冲槽口状态设置模块，设置请求操作的槽口状态和队列号，分配空闲槽口；缓冲槽口滤除模块，滤除不属于当前操作队列且非空闲态的槽口状态，按头指针对齐；队列槽口选择模块，计算头指针指向槽口起的连续空闲槽口数并更新头指针，选出预备态的槽口；队列槽口优先排队模块，用第一个预备态槽口的指针和预备态槽口数分别更新当前操作队列的读指针和结果数；本发明可以支持多个队列共享一个缓冲空间，各类指令队列能对缓冲进行交叉访问，并对指令结果的写入读出进行顺序化管理。

The result was as much a history (in the modern meaning of the word) as a theory, in that Burnet transformed the hypothetical cosmogony and geogony of René Descartes into a sequence of irreversible events, documented by both nature and Scripture.

伯内特把笛卡尔假设的宇宙形成理论和地球形成理论转换成了既由自然界又由圣经记录的一系列不可逆事件，这样，结合的结果既是一种理论，又同样多地是一种历史（" history "的现代意义）。

During the initialization sequence the bus master transmits the reset pulse by pulling the 1-Wire bus low for a minimum of 480μs.

在初始化步骤中，总线上的主机通过拉低单总线至少480μs来产生复位脉冲。

The recombinant plasmid containing gAd gene was transformed into E. coli BL21(DE3), and the expression of the fusion protein His-ad was induced by IPTG. Results The gAd cDNA of 412 bp was obtained from the total RNA of the fat tissue and verified by sequence analysis.

结果 从脂肪组织总RNA中扩增得到412 bp片段gAd基因，其cDNA序列与GenBank人gAd基因序列相同，并在体外表达得到相对分子质量为34 000的重组蛋白。

The main conclusions are as following:(1) Befor the frozen depth reach to the maximum, the frozen depth of different treatments of groundwater, 0.6% of calcium nitrate, 0.6% of potasium sulphate and no irrigation are 132 cm, 130 cm, 129 cm and 108cm in sequence respectively.

得到的主要结论如下：(1)不同冬灌水质处理在冻深达到最大以前，采用地下水灌溉的处理最大冻深最大，为132cm，冻结速率最高，为1.16cm·d~(-1)；加入0.6%的硝酸钙的处理最大冻深次之，为130cm，冻结速率为1.13cm·d~(-1)；加入0.6%的硫酸钾的处理最大冻深排第三，为129cm，冻结速率为1.10cm·d~(-1)；自然条件下未灌溉处理的最大冻深第四为108cm，冻结速率为0.94cm·d~(-1)。

It simulates the circuit breaker with a zero impedance branch, and adopts the linear network characteristic and theory, variable structure and variable parameter analyses to transfer the short-circuit current flow in the bus-bar equality into each side of circuit breaker, and deduces the sequence component model and algorithm for calculating the short-circuit current of circuit breaker when the short-circuit fault occu...

该方法将断路器作为一条阻抗为零的支路来模拟，运用线性网络特性、定理及变结构与变参数的分析方法，将母线上短路时的短路电流等值转移到断路器两侧，导出了相当于在断路器两侧分别发生短路故障时，流过断路器的短路电流的序分量模型和算法。

You can see, there is Sequence activity named as Calculator inside of the Try.

你可以看到，有顺序的活动命名为计算器内部的尝试。

Tarski's definition of truth of the calculus of classes is: x is true if and only if x is a sentence of the calculus of classes and every infinite sequence of classes satisfies x .

塔斯基得到的真概念定义是：x是类演算语言的任一语句，x为真当且仅当每一个类的无穷序列都满足x。

Aiming at the different types of basins developed in Caledonian period in the region, the sequence stratigraphy filling models of the different type basins are established.

针对研究区内在加里东期发育的不同类型盆地，建立了不同盆地类型的层序地层充填模型。

For the head-teacher of the class said that I had seriously broken the school rules,which led me to a three-day suspend class.

为什么呢？因为我的班主任说我严重的违反了校规，于是让我停课三天。

Many of them believe that the conversion of thousands of working-class folk in England spared that nation from the mass carnage and the tyranny that came with the revolution in France.

他们之中有许多人相信，在英国数以千计的劳工阶级之悔改信主使英国免於遭受如法国大革命所造成的大屠杀和专制暴政。