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Bluetongue virus ; S7 gene ; molecular cloning ; sequence analysis

蓝舌病毒； S7基因；分子克隆；序列分析

The cloning of bar gene is important to plant transgenic engineering, gene expression and studying physiology.In the experiment, the full code sequence of bar gene was cloned by PCR from transgenic herbicide resistant Bobwhite wheat and checked. It was expressed in E.coli and its protein was determined.

本实验从抗除草剂转基因Bobwhite小麦中，利用PCR克隆的方法扩增出bar基因全长，并在原核表达系统中表达，鉴定表达蛋白的活性，将能够正确编码PPT乙酰转移酶的bar基因片段，经过适当的修饰构建入真核表达载体。

Rice OsbolA1 gene encodes a predicted protein with 167 amino acids showing 52% sequence identity with Escherichia coli BolA protein.

水稻OsbolA1 cDNA含501bp的ORF，编码167aa，推导的氨基酸序列与大肠杆菌BolA有52％的同源性。

His favourite was a sequence of cooking a rhea--a short-legged, ostrich-like bird--which he had felled with the classical hunting weapon of Patagonia, the bolas.

他最喜欢烧烤三趾鸵鸟的一套活计。这种鸟腿短，样子像鸵鸟。他用巴塔哥尼亚传统的狩猎武器流星锤击倒猎物。

The first part is the research of identification of vitellogenin and analysis of its cDNA sequence, and research of composition of yolk protein in Bombyx mandarina Moore.

第一部分是野桑蚕卵黄原蛋白的鉴定与cDNA序列的分析，以及卵黄组成的研究。

The Workbook has six-page units that follow the same sequence as the Student's Book, recycling and reviewing language from previous units.

工作簿已长达六页的单位，遵循与学生的书相同的序列，回收和审查以前单位的语言。

In this paper we construct Faure sequence with modulus 2 through Boolean operation.

介绍一种利用逻辑运算构造Faure序列的方法，尤其是当模2时的该序列的构造。

The device has the capability to protect the data in the boot block,this feature is enabled by a command sequence.

该装置有能力保护引导块中的数据，此功能是由一个命令序列启用。

The device has the capability to protect the data in the boot block; this feature is enabled by a command sequence.

该装置有能力保护在引导块中的数据，这个特点是由一个命令序列启用。

X60008EIS8-50 Pinout: In order to detect whether the boot block feature is set on or not, users can perform software command sequence: enter the product identification mode (see Command Codes for Identification/Boot Block Lockout Detection for specific code), and then read from address 7FFF2.

X60008EIS8-50引脚说明：为了检测是否启动区块功能或没有设置，用户可以进行软件的命令序列：输入产品识别模式（见识别/引导块锁定检测特定的代码指令代码），然后从地址7FFF2阅读。

For the head-teacher of the class said that I had seriously broken the school rules,which led me to a three-day suspend class.

为什么呢？因为我的班主任说我严重的违反了校规，于是让我停课三天。

Many of them believe that the conversion of thousands of working-class folk in England spared that nation from the mass carnage and the tyranny that came with the revolution in France.

他们之中有许多人相信，在英国数以千计的劳工阶级之悔改信主使英国免於遭受如法国大革命所造成的大屠杀和专制暴政。