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The regulative sequence of ribulose-1 ,5-bisphosphate carboxylase small subunit genes of rice was cloned by PCR amplification.

利用PCR法克隆了水稻日本晴来源的核酮糖-1、5-二磷酸羧化酶小亚基基因5'上游调控区，命名为Posrbcs。

Cardinal numbers: Normal sequence of numbers, one, two, three, as compared with ordinal numbers, first, second, third, etc.

基数：数字的常规次序，如一，二，三等；以别于，如第一，第二，第三等。

Two of the distinctively Venetian institutions known as the Scuole retain some of the outstanding examples of Venice Renaissance art - the Scuola di San Rocco , with its dozens of pictures by Tintoretto, and the Scuola di San Giorgio degli Schiavoni , decorated with a gorgeous sequence by Carpaccio.

威尼斯两个特殊的协会，一个是Scuole保留了一些文艺复兴时期的杰出作品－－圣马可Scuola，Tintoretto拥有许多画，这些都可以把卡帕奇欧装饰得异常华丽。

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列，采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC，将其转染胃癌BGC-823细胞，应用Western blot和免疫组织化学法检测yrdC蛋白表达，设对照组和转染Ad-Null组，并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究：通过RFLP及微卫星多态性分析，应用两位点及多位点连锁软件，建立了中国人WD基因在D13q14.2-3区域的精细遗传连锁图谱，从而首次对中国人WD基因进行了精确定位；②WD基因突变研究：应用PCR-SSCP及DNA测序技术，对39个家系45名WD患者进行该致病基因的21个外显子突变筛选，发现WD基因5号外显子存在新的T插入突变，并证实中国人WD基因的突变热点为8号外显子，突变形式为Arg778Leu，其频率为22.8%；③WD的症状前诊断和杂合子检出：应用DNA重组技术对79个家系进行基因诊断，成功地进行了WD的症状前诊断和杂合子检出，并建立了WD的基因筛选的PCR-Msp 1酶切方法。

In this thesis, we gives a novel graphical representation of DNA sequences by taking four special vectors in 2-D Cartesian coordinate system to represent the four nucleic acid bases in DNA sequences, so that a DNA sequence is denoted on a plane by a directed walk.

由于在DNA计算中，信息总是通过特定长度的DNA序列来表示的，因此，选择高质量的方式来表示DNA序列就成为一个重要的问题。

The sequence of poems began with the Song of the Torches , which tells in a vision how a spiritual army will liberate men and women whose feelings have been deadened, whose spirits have been confined, and on whose mouths "there is carven a hopeless despair".

下面这个系列的第一首是《火炬之歌》，它描绘了这样的景象：精神的力量终将使那些"情感像冰一样凝固的/血像冰一样冷的/嘴角雕着失神的绝望的"人们，获得自由。

DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

During the planning process, the CATT discussed the future development of Tamsui Township not only with local culture organizations, but also with community residents. Meanwhile, the team also pushed the county and township government to start a sequence of real projects renovating the living space, for example, as the planning project of bikeway along Tamsui river bank, the rebuilt project of Shih-Cyue Do-Tou public toilets, and the renovation project of plaza and market in front of Fu-You Temple. In 1994, CATT proceeded with historical space renovation plan among cultural heritage in both Tamsui and Hsin-Kang, Chiayi County followed by the "Community Empowerment Plan" from Council for Cultural Affairs, Taiwan.

在规划过程中不仅与淡水在地的文化团体及社区居民共同讨论市镇未来的发展方向，同时也促成县政府与镇公所开始进行生活空间的实际改造工程（如，淡水河岸自行车道规划设计、屎礐渡头公厕改建案与福佑宫前庙埕与市场改建案）。1994年，参与行政院文化建设委员会的社区总体营造计画，推动了淡水和嘉义新港两地的传统文化建筑空间改造计划。

Total RNA was extracted from flowers of cattleya, according to the conserved acid sequence for ACC oxidase in other orchids, we designed a pair of oligo nucleotide primers. Using RT-PCR method, a cDNA fragment about 967 base pair, which encoded 321 predicted amino acid residues, was amplified.

以卡特兰花瓣为试材，提取其总RNA，并根据其他兰花的ACC氧化酶(1-aminocyclopropane-1-carboxylic acid oxidase， ACO)基因保守序列设计一对特异性引物，通过RT-PCR法克隆得到1条967bp的卡特兰ACO cDNA片断，共编码321个氨基酸残基。

Among which is the mindset, perceptions and moralities of those who accept mini- military tasks for economic gains.

其中最重要的是心态，观念和道德的人谁接受小型军事任务的经济收益。

In time course imaging, activity peaked 2 days after coronary ligation.

在时程成像检测，活性峰值在冠脉结扎后2天。