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To evaluate the effect of cold stress on hypothalamic-pituitary-adrenal HPA axis in chic-kens,15-day-old male Isa chickens were subjected to acute(for 15min,1h,3h,6h,12h and 24h,respectively) and chronic(for 5d,10d and 20d,respectively) cold stress at 12℃±1℃.The level of expression of corticotropin releasing hormone mRNA in hypothalami and the contents of adrenocorticotrophic hormone,aldosterone and cortisol in serum of the chickens were detected.
为探明冷应激对雏鸡下丘脑-垂体-肾上腺皮质轴的影响,对15日龄公雏鸡进行了急性(15min、1h、3h、6h、12h和24h)与慢性(5d、10d和20d)冷应激处理(12℃±1℃),检测了雏鸡下丘脑促肾上腺皮质激素释放激素mRNA的表达水平及血清促肾上腺皮质激素、醛固酮及皮质醇的含量。
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The height of turfgrass and the length of the second leaf from the top were shown to significantly increase under the coring aerification, vertical mowing and raking, treatments compared with the control treatment, with the height of turfgrass increased by 34.64%,27.71% and 39.26%, respectively, and length of leaf increased by 24.03%,17.05% and 31.78%, respectively.
结果表明,打孔、切根和梳草3种措施对夏初修复的草坪草层高度和叶片长度有明显的促进作用(P〈0.05),草层高度依次比未修复+未施肥处理增加34.64%、27.7l%和39.26%;叶片长度依次增加24.03%、17.05%和31.78%。
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The carbonaceous alcosol and the silica alcosol were prepared respectively by using aqua-mesophase and tetraethoxysilane as starting materials. Then the two alcosols were mixed and dried in open air and under supercritical fluid conditions to form binary carbonaceous-silica xerogel powders and aerogel powders respectively.
以正硅酸乙酯和炭质水性中间相为原料,分别制得了炭质醇溶胶和硅醇溶胶,将二者进行混合后再利用常压干燥法和超临界流体干燥法分别制备了二元炭质-二氧化硅干凝胶和气凝胶粉末。
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Materials and methods: The composite was respectively prepared by blending Lysine, Histidine, Arginine with PLGA, while the composite respectively prepared by blending Algin, chitosan, NaHCO3 with PLGA as the comparison and the pure PLGA as the control. All composites were degradated in tri-distilled-water at 37℃ for 7 weeks. The degradation property of PLGA with the addition of basic additives was investigated by using gross observation, the pH change of degradation solution and the mass loss ration of the composite.
材料方法:将赖氨酸、组氨酸、精氨酸分别各按5%和10%的比例与PLGA制成复合物,于体外在37℃下在三蒸水中进行降解2个月;通过总体观察、降解液PH的变化、复合物的失重率等指标来考察碱性氨基酸对PLGA体外降解特性的调节作用,同时将碱性氨基酸的调节效果与海藻酸钠、壳聚糖、碳酸氢钠的进行对比。
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Quinclorac and allyl alcohol as raw materials, 3, 7-dichloro-8-quinolinoxylic acid allyl ester was obtained when catalyst is oil of vitriol or super solid acid SO〓/TiO〓. Using both catalysts, organic synthesis technology process was made respectively, and the ester was analyzed and characterized by IR spectrum and NMR spectrum Through experiment, both preparing conditions were optimized and discussed respectively.
以TiO〓为载体负载SO〓制备出固体超强酸催化剂,确定了SO〓/TiO〓催化剂制备的技术路线,以丙酸正丁酯为探针实验,考察了新型固体酸催化剂SO〓/TiO〓的催化活性及制备条件对催化活性的影响,对影响其催化效果的工艺条件进行了优化,并探讨了影响的规律性。
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ResultsThe IC50 of the ethyl acetate extract from Alocasia macrorrhiza to A549, B16, BGC-823 were 94.6, 541.9,629.5 μg·ml-1 respectively,and those of the acetone extract from Alocasia macrorrhiza to A549 and B16 40.9,438.0 μg·ml-1 respectively. But there were quite large dosages of the IC50 of the extracts of cyclohexane, benzine, ethyl alcohol and water from Alocasia macrorrhiza to all kinds of cell lines.
结果海芋醋酸乙酯提取物对A549,B16,BGC-823的IC50分别为94.6, 541.9,629.5 μg·ml-1,丙酮提取物对A549,B16的IC50分别为40.9,438.0 μg·ml-1,而海芋的环己烷,石油醚,乙醇,水提物对各种细胞株的IC50剂量都比较大。
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He owned a luxurious hotel on the Riviera . His father and two grandfathers had sold wine, jewels and silk, respectively. At thirty he married an English girl, daughter of Jerome Dunn, the alpinist, and granddaughter of two Dorset parsons, experts in obscure subjects — paleopedology and Aeolian harps, respectively.
我的父亲在拉文纳拥有一家豪华的饭店,他的父亲、祖父和曾祖父分别做过酒、珠宝和丝绸生意。30岁的时候他娶了一个英国姑娘,登山家杰罗姆·登的女儿,两位多赛特牧师的孙女,他俩分别精通极冷僻的古土壤学和风奏琴。
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The installed capacity in terms of soda ash and ammonium chloride respectively contributed by its shareholders accounts for 77% and 73% respectively of national aggregate capacity.
公司股东的纯碱和氯化铵生产能力分别占全国总能力的77%和73%。
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Results There were fetal type in 12 cases, embryonal type in 7 cases, anaplasia type in 3 and mixed type in 2. The cytoplasmic postive expression of CK, AFP, S-100 protein and Vimentin of epithelial tumor cells were found in 14, 10, 9 and 6 cases respectively. Positive staining of CEA, P53 and P16 of nuclei of epithelial tumor cells were found in 11, 9 and 7 cases respectively.Among the 12 cases who underwent surgical excision, 8 patients with fetal type survived over 5 years. Conclusions This study suggests that immunoreaction of hepatoblastomas are different in the histological subtypes.
结果 胎儿型12例,胚胎型7例,间变型3例和上皮间叶混合型2例;细胞角蛋白、甲胎蛋白、S-100蛋白和波形蛋白在肿瘤上皮细胞浆的表达分别为14例、10例、9例和6例,癌胚抗原、P53和P16蛋白在细胞核中的表达分别是11例、9例和7例;手术切除肿瘤12例中8例生存期超过5年者均为胎儿型。
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Pyroxene grains in gabbroic anorthosite,troctolite,microporphyritic crystalline impact melt breccia,and Ti-bearing clasts are mainly low-Ca pigeoniteEn_(63Fs_(32)Wo_5,En_(66)Fs_(23)Wo_(11),En_(55)Fs_(38)Wo_7,and En_(57)Fs_(35) Wo_8,respectively,and those in anorthositie gabbro,gabbro,and the matrix display a wide range of compositionEn_(31~33Fs_(35~48) Wo_(19~34),En_(18~48)Fs_(23~51)Wo_(24~38)+En_(1~9)Fs_(65~86)Wo_(14~26),En_(11~53)Fs_(23~63)Wo_(6~36),respectively,mainly occurring as pigeonite,augite, and trace amounts of pyroxferroite.
辉长质斜长岩、橄长岩、微斑熔融角砾岩等岩屑和含钛玻屑中的辉石相对贫钙,主要为易变辉石(分别为En63 FS32 WO5,En66FS23WO11,En55FS38Wo7和En57FS35WO8),而斜长岩质辉长岩和辉长岩岩屑中的辉石和晶屑辉石的成分变化比较大(分别为En31~33 FS35~48 WO19~34,En18~48 FS23~51 WO24~38+En1~9 FS65~86 WO14~26和En11~53 FS23~63WO6~36),为易变辉石和普通辉石,以及少量三斜铁辉石。
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Some of them were ligated to the plasmid vector pCR 2.1 andsequenced,respectively.
将所获DNA片段克隆到pCR2.1载体进行测序,并与基因组数据库进行同源性比较。
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Thinking since Google owns Blogger, I would be able to escalate this issue to the support team.
自谷歌的思考拥有Blogger的,我将能够缓解这一问题的支持团队。
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Besides, I also surely know that just professional skills are not enough for being a good doctor. Hence, I attend the basic scientific research of preclinical medical college, the epidemiological investigation of public health college and the clinical scientific research of clinic college,from which I possess of some experimental skill such as CELL CULTER , PCR , Western blotting and so on.Above all,these experience cultivate my ability of designing the experiment and enforcing the experiment,which help me complete my research.
做为一名七年制的学生,学校对我们的要求是&临床技能和科研能力同等重要&因此在进行临床技能培养的同时,我先后参加过我校基础医学院的基础科研实践、公共卫生学院的流行病学调查实习,及临床学院的临床科研实践,让我熟练掌握了细胞培养、 PCR 、 Western blotting 等分子生物学技术;最重要的是,这些实践经验培养了我独立完成从研究课题的设计到实验方案的实施能力,帮助我顺利的完成了我在硕士期间的科研项目。