查询词典 polymerase

Of the polymerase and allows it to proceed. The octamer then binds to the DNA behind the polymerase and no longer presents an obstacle to progress.

图27.36：RNA聚合酶在前进中会取代DNA的组蛋白八聚体，DNA恢复成环并附着，组成一个狭窄的环。

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷（5-Aza-2'-dc，以下简称Aza）及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后，用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况，用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况，并用逆转录聚合酶链反应检测ING1bmRNA表达。

The result would be used in tobacco breeding.4 SRAP technology is used to study the genetic differences among different types of tobacco, including flue-cured tobacco, oriental tobacco, maryland tobacco, burley tobacco, and air/sun cured tobacco, five pairs of primer combinations which can amplify characteristic bands has been screened. Among the five combinations, two of them (SF25*SR2 and SF3*SR26) can distinguish air-cured tobacco including maryland tobacco and Burley tobacco from the other cultivated forms; SF20*SR14 can distinguish sun cured tobacco including oriental tobacco and sun-cured tobacco; SF16*SR14 can distinguish flue-cured tobacco and air-cured tobacco, and SF22*SR14 can distinguish DBJ599 with specisl aroma and the other flue-cured tobacco.5 8 varieties , which represent the different types of tobacco, are divided into 3 groups. I: G-28, II:DBJ599、MD40、By21, III: Basma、Sumsun、XNS、QCT. The cluster analysis result es consistant with experience evatuation.6 Anorthogonal design was used to optimize a ILP-PCR system with 5 factors (DNA, Mg2+, dNTP, primer and Taq polymerase) at 4 levels.The result showed the optimized ILP-PCR system for Nicotiana tabacum L. was: 2uL10×PCRbuffer, 20ng template DNA, Mg2+ 2.0mmol/L, dNTP 150umol/L, primer 0.3umol/L, Taq DNA polymerase 1 U in a total of 20uL reactionsolution.

研究结果可作为烤烟与香料烟类型间的杂种鉴定利用的实验与理论依据。4、利用SRAP标记技术，研究和分析了烤烟、晒烟、晾烟、白肋烟、香料烟等不同栽培类型间的基因型差异，初步筛选出了能够在分子水平上反映不同烟草类型差异的特征性标记带：晾烟MD40、Ky17、Burley21、TN90基因型共有的特征带有两对引物组合，分别为SF25*SR2和SF3*SR26；晒烟小牛舌、青梗、小花青和香料烟Sumsun、komotini Basma、Xanthi Basma共有的特征带引物组合为SF20*SR14；NC89、K326、G-28、大白筋和MD40、Ky17、Burley21、TN90共有的特征带引物组合为SF16*SR14，烤烟中大白筋599的特征带引物组合SF22*SR14.5、利用61对SRAP引物组合，研究了普通烟草不同类型间的亲缘关系，将普通烟草类型间8个不同供试材料划分为3类别。I：烤烟类型G-28II：白肋烟类型(MD40、By21、大白筋599III：香料烟类型(Basma、Sumsun、小牛舌、千层塔)。

Effect of TP on DNA primase-polymerase α complex activity was examined using Liquid Scintillation counter. Results:It showed that IC50 value of TP inhibiting DNA primase-polymerase α complex activity was 20.28 (15.17～ 27.13)μ g/ml. TP could promote DNA primer synthesis but inhibit DNA primer extension.

结果：茶多酚对小鼠肿瘤L2细胞DNA引物酶多聚酶α复合体活性有抑制作用，半数抑制浓度（IC50）值为20.28(15.17～27.13)μg/ml；对DNA引物合成有促进作用；对引物延长亦有明显抑制作用，其IC50值为36.43μg/ml。

Among analyzed 197 synthetic genes on chip, approximately 60 genes have been expressed, including 18 two-component/kinase, 10 glycolysis/TCA, 3 gene clusters, 7 regulators, 5 asaption/celldivision/differentiation/sporulation, 4 transport/binding proteins, 2 chaperones, 5 RNA polymerase sigma-factors, 3 transcription/translations, 2 fatty acid synthesises and 1 RNA polymerase β subunit.

对芯片上的197个合成基因进行分析：大约有60个基因进行了表达，其中包括18个双组分或激酶基因；10个糖酵解或者三羧酸循环途径基因；3个阿维基因簇；7个调节基因；5个细胞分化或孢子形；4个运输或固定基因；两个伴侣分子；5个RNA聚合酶σ因子；3个转录基因；两个脂肪酸合成基因；1个RNA聚合酶β亚基。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证明。

On the other hand, flow cytometry was employed to detect the action of paclitaxel to cell cycle and apoptosis. Reverse transcription polymerase chain reaction is employed to detect the influence of paclitaxel on Survivin mRNA expression. The purpose of the study is to provide theoretical basis to carcinogenesis, progress, metastasis, angiogenesis and clinical chemotherapy of paclitaxel on TSCC.

采用流式细胞仪检测紫杉醇对舌癌细胞细胞周期的影响及应用逆转录聚合酶链反应(reverse transcription polymerase chain reac-tion，RT-PCR)检测紫杉醇对舌癌中Survivin mRNA的影响，为研究舌鳞癌的发生、发展机制，舌癌的转移及新生血管形成，及临床应用紫杉醇抗舌癌提供理论依据。

Polymerase chain reaction ; agglutinin ; pneumonia ; mycoplasma

聚合酶链反应；凝集素；肺炎；支原体属

Glossy ganoderma decoction may protect the liver from Amanita mushroom poisoning. Its mechanism may be related to the increase of the activities of hepatocyte RNA polymerase.

灵芝煎剂对鹅膏毒蕈中毒肝有明显保护作用，其机制可能与提高肝细胞RNA多聚酶活性有关。

I want to be a big fish in a small pond.

我要做一条在小池塘里的大鱼。

While the software functioned well, we did not feel the same about their customer support!

虽然该软件运行良好，我们没有同样的感觉他们的客户支持！