查询词典 polymerase

F508 mutation detection on the single blastomere was performed with nested polymerase chain reaction.

同时利用套式聚合酶链反应技术，对活检所得的单个卵裂球分别进行ΔF508 突变基因检测。

BOULE transcript levels in the testes of 41 azoospermic patients were examined by quantitative competitive-reverse transcription-polymerase chain reaction.

我们以定量竞争性反转录聚合连锁反应的方法定量 41 位无精虫症患者睪丸内 BOULE 的转录量。

Characterization of complementary DNAs derived from braconid wasp ovaries identified genes encoding subunits of a viral RNA polymerase and structural components of polydnavirus particles related most closely to those of nudiviruses—a sister group of baculoviruses.

基因编码的RNA聚合酶亚组和多去氧核糖核酸病毒粒子的结构组件，与nudiviruses有着密切的联系。而通过来自小茧蜂卵巢互补DNAs的表征可以将两者识别出来。

Besides its simpliness and quickness, the method also has characters of specificity and sensitivity. It has a great importance to detect and diagnose Brucellosis by Polymerase Chain Reaction.

该奶牛布鲁氏菌病病原PCR快速检测方法的建立，对奶牛布鲁氏菌病的检疫及快速诊断具有十分重要的意义。

Methods The experimental TBI model was established by bumpiness of free falling body according to Feeney′s.The rats′cerebral edema were imaged with magnetic resonance image,The changes of brain water content and permeability of blood-brain barrier were measured by the methods of wet and dry weight and Evans blue fluorometry.The expression of AQP-4 mRNA was examined by reverse transcriptase polymerase chain reaction.

按照改进的Feeney自由落体撞击法建立大鼠创伤性脑损伤模型，用磁共振成像对大鼠脑水肿进行检测，干/湿比重法和伊文思蓝测定法观察大鼠TBI后不同时相脑组织含水量和BBB通透性的变化，并采用逆转录聚合酶链反应法检测AQP-4 mRNA的表达，蛋白质免疫印迹法检测AQP-4蛋白的表达。

After synthesis of the DNA polymerase and replication of DNA,the viral capsid and glycoproteins are synthesized.

在DNA多聚酶合成DNA复制后，病毒衣壳和糖蛋白合成。

The expressions of HO-1 and HO-2 mRNA were detected using reverse transcription polymerase chain and the relative amount of CO released into the media was quantitated as carboxyhemoglobin by enzyme linked immunosorbent assay.

通过逆转录聚合酶链式反应和免疫细胞化学法检测HO-1、HO-2 mRNA和蛋白的表达，酶联免疫法检测培养上清液中碳氧血红蛋白含量，四唑氮比色法检测细胞增殖。

DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

Polymerase chain reaction analysis of cerebrospinal fluid is the gold standard for the diagnosis of herpes simplex encephalitis.

对脑脊液的聚合酶链反应是诊断单纯疱疹性脑炎的金标准。

This paper designs the communication system of radio telescope FAST.

针对500m口径球面射电望远镜FAST分布式控制系统的特点完成了其通信系统的设计。

Political sheep's step-by-step a large number of fossils unearthed in the county soil Ma Ali after rural village in the middle of sallow pelitic siltstone.

步氏和政羊化石大量出土于广河县阿力麻土乡后山村，产于灰黄色泥质粉砂岩中。