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The profilin of Caryota mitis pollen was cloned and expressed successfully in E. coli BL21 (DE3), which will be used for further study on Caryota mitis pollen related allergy.

成功地克隆和表达了短穗鱼尾葵花粉profilin，为短穗鱼尾葵花粉过敏的诊断和免疫治疗奠定了基础。

Pollen allergens from Carvota mitts and Caryota ochlandra were purified and characterized. The result will provide a basis for the diagnosis and treatment of allergy to the pollen allergens from Carvota mitis and Caryota ochlandra.

对长穗和短穗鱼尾葵花粉变应原进行了初步的分离、鉴定和纯化，为临床鱼尾葵花粉变态反应疾病的诊断和治疗奠定了基础。

The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物，采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框，将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达，通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化，采用Western-blot检测其IgE结合活性。

IgE reactivity of recombinant Caryota mitis pollen profilin was investigated by western blot. Results The Caryota mitis pollen profilin gene was cloned. The cloned fragment was 608bp in length, with an 396bp open reading frame coding for 131 amino acids.

结果 克隆获得了短穗鱼尾葵花粉profilin的全长基因，由608个碱基组成，开放阅读框为396个碱基，编码131个氨基酸。

In this thesis, using Pinus yunnanensis and Nicotiana tabacum pollen as experimental material, we studied the regulation and control of calcium messenger system in pollen germination and tube growth.

我们以云南松和烟草为材料，试图阐明钙信使系统对花粉萌发和花粉管生长的调控。

Shortly after the pollen grain comes in contact with the nucellus, it germinates to form a pollen tube.

在不久之后，花粉谷粒用 nucellus 进来连络，它发芽形成花粉管。

10The simulations of an empirical model indicated that, when the outcrossing rate was no more than 1%, a 2 m buffer area with pollen receiver individuals between pollen donor and receiver was sufficient to minimize the frequencies of gene flow to .9%.

对栽培稻到其野生近缘种的基因漂移进行模拟的结果表明，栽培稻到野生稻的基因漂移频率较高并且可能会对邻近的较大范围内的野生近缘种群体造成影响。

The model simulations indicated that, when the outcrossing rate was no more than 1%, a 5 m buffer area with pollen receiver individuals between pollen donors and receivers was sufficient to minimize the frequencies of gene flow to .9%.

然而，对栽培水稻到其野生近缘种的基因漂移进行模拟的结果表明，栽培水稻到野生稻的基因漂移频率较高并且可能会对邻近的较大范围内的野生近缘种群体造成影响。

To further address how OSCK1 affect the pollen germination process, plasmids which can overproduce GFP-fused OSCK1 proteins were bombarded into the germinating lily pollen.

为了进一步了解OSCK1如何影响花粉管萌发，利用基因枪将融合GFP之OSCK1质体送入正在萌发的百合花粉管中，进行暂时性基因表现分析。

In this paper, we studied the relationships between pollen histochemistry, pollen grain number, ovule number and pollinators in Phyllanthaceae.

本文研究了叶下珠科部分种类的花粉组织化学、花粉数和胚珠数，以及它们与传粉者之间的关系。

We are forbidden from giving money to these shoeless, skinny children, as this will perpetuate begging.

向光着脚，骨瘦如柴的孩子施舍是不可以的，因为要招来无休无止的乞讨。

It would be hard not to fall victim to some sharpeyed policeman.

要想不被某个目光锐利的警察抓到，谈何容易。