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passaging相关的网络例句

查询词典 passaging

与 passaging 相关的网络例句 [注:此内容来源于网络,仅供参考]

Methods The mouse uterus of 1-3 days implantation embryos were separated, sheared and cultivated. Inner cell masses were dissociated and passaged in two days according to routine method of ES cells digesting and passaging.

分离小鼠胚胎着床初期1~3天(孕4.5~6.5天)小鼠子宫,剪碎后进行组织块培养,2天内分离内细胞团,以后按常规的胚胎干细胞培养及传代方法进行传代。

An equine infectious anemia virus donkey adapted strain, which has lethal virulence to horse and donkey, was obtained through serials passaging in donkeys in vivo used a wide-type EIAV isolate.

马传染性贫血病毒(equine infectious anemia virus, EIAV)驴强毒株DV是一株经过驴体传代获得的而具有超强毒力的毒株,对马和驴均可100%致死。

Methods The astrocytes were isolated from cerebral cortex of neonatal rats which are born within three days, cultivating by DMEM media added with 15% fetal calf serum and depurating by swinging and passaging, to simulate nulli-nutrition model by substituting medium for PBS buffer solution 3h then applying methylprednisolone and recnutrition immediately, we will go on culturing for three days, verificating astrocytes by immunofluorescent technique; detecting activity of AST; determining GFAP of change by PCR.

采用出生3d内的SD大鼠的新生鼠的大脑制成细胞悬浊液后,应用含15%胎牛血清的DMEM培养液中培养,通过摇床和逐渐传代纯化星形胶质细胞,通过PBS缓冲液代替培养基模拟缺营养模型3h后即刻应用甲基强的松龙(10umol/l),然后继续培养3d,免疫荧光技术鉴定星形胶质细胞;MTT法检测细胞的增殖活性和凋亡;PCR技术测定细胞GFAP表达的变化。

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