查询词典 passaging

Results The lymphatic metastatic rates were higher than 85%.The metastatic paths were single, the proliferation ability was increased in cancer cells of every passage in vivo and vitro after passaging cancer cells of lymphatic metastatic foci for 9 cycles in nude mice.

结果 癌细胞在裸鼠体内传9代后，各代裸鼠的淋巴结转移率一直稳定在85％以上，且转移途径较为单一；各代癌细胞的体内外增殖能力不断增强。

After natural purification and three passaging, we detected CD90 expression in fibroblasts grown on cover glass by SABC immuocytochemistry techniques.

经过3次传代、自然纯化后得到性质稳定的成纤维细胞，冻存后复苏传1-3代，制成细胞爬片，免疫细胞化学SABC法染色检测CD90的表达情况。5。

In serial passaging experiments, the genome of the chimeric pHBIV-3 could not exist stably. The gag or tat mRNA and the activated proteins could not be detected.

在MT〓细胞中，由于BIVLTR在人细胞中的基础转录活性较弱，尽管存在bTat-bTAR同源反式激活系统，仍不能产生感染性病毒。

These spheres grew gradually as the culture time extended and the diameter reached to 1mm after 3 weeks of culture. These spheres could be continuously passaging in DMEM/F12 + bFGF + EGF.

随着培养时间延长，微球体直径逐渐增大，3周左右可达到1mm左右，在体外可连续传代，形成新的微球体。

Viruses do 'escape' from laboratories, even high security facilities. The H1N1 influenza lineage that circulated in the human population for four decades after the 1918 Spanish influenza epidemic, disappeared during the 1957 Asian influenza pandemic, was absent for two decades, but then reappeared in 1977. Gene sequences of the 1977 isolate and others collected in the 1950s were almost identical, indicating that the virus had not replicated and evolved in the interim, and had probably been held in a laboratory freezer between 1950 and 1977 and 'escaped' during passaging.

病毒确实从实验室中'逃跑'，即使有高度保护设施。H1N1家族流感病毒，在人类传播了40年自1918年西班牙流感疫情之后，在1957年亚洲流感大流行后失踪，消失了20年，但随后在1977年重新出现。1977年分离基因序列和在20世纪50年代收集的基因序列几乎相同，这表明病毒并没有复制和演变在中间期，并可能在1950年至1977年保存在实验室冷冻器，又在中间期内&逃脱&出来。

Results In contrast with enzymatic dissociation, the method of mechanical dissociation, breaking up great neurospheres into small spheres with gradually reduced pipette, had advantages of raising cell viability and shortening cell doubling time (P.05). After NSCs being cultured long term in vitro, the number of neurons generated decreased with passaging, whereas the number of astrocytes increased.

体外长期培养条件下，随着培养时间的延长，NSCs分化为神经元的比例逐渐降低，星形胶质细胞的比例逐渐增高；在4~12代NSCs分化为神经元的能力表现出下降的趋势，但仍较为稳定；12代后，神经元的分化比例迅速下降，星形胶质细胞的比例迅速上升。

The H1N1 influenza lineage that circulated in the human population for four decades after the 1918 Spanish influenza epidemic, disappeared during the 1957 Asian influenza pandemic, was absent for two decades, but then reappeared in 1977. Gene sequences of the 1977 isolate and others collected in the 1950s were almost identical, indicating that the virus had not replicated and evolved in the interim, and had probably been held in a laboratory freezer between 1950 and 1977 and 'escaped' during passaging.

病毒确实从实验室中'逃跑'，即使有高度保护设施。H1N1家族流感病毒，在人类传播了40年自 1918年西班牙流感疫情之后，在1957年亚洲流感大流行后失踪，消失了20年，但随后在1977年重新出现。1977年分离基因序列和在20世纪50 年代收集的基因序列几乎相同，这表明病毒并没有复制和演变在中间期，并可能在1950年至1977年保存在实验室冷冻器，又在中间期内&逃脱&出来。

MSCs from human BM were isolated by Ficoll density centrifugation cultured and expanded in DMEMLG containing FBS. MSCs are adherent and fibroblastic, and maintained similar morphology as passaging.

从人骨髓中分离和培养间充质干细胞，在原代和传代培养中其形态学上均为贴壁、纤维状的细胞，随著代次的增加细胞形态更加趋於一致。

Methods: the cells are isolated by two-step enzyme digestion and purified by the Percoll density centrifugation,the proliferation and differentiation propertiesof which being observed.To study the effect of IGF-1 on satellite cell with MTT method and obtain adequate single satellite cell by frequent passaging.

运用两步酶消化法分离卫星细胞，不连续密度梯度离心法纯化后体外培养，观察其生长与分化特性，运用MTT法测定卫星细胞对不同浓度IGF刺激的反应，并运用频繁传代的方法获取足量单个肌卫星细胞。

Passaging S79-1-3 strain in CEC cell cultures may result in selection of its substrain compositions, S79-1-3 strain was less stable than S79-1-P7-3③ strain.

结论S79株病毒与文献报道的JL2株十分相似，经连续传代总体的核苷酸差异〈0.5％。S79-1-3主代病毒在药典规定代次范围内甚为稳定。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。