查询词典 passaging

SCs cultured from rabbit skeletal muscle can grow and maintain their characteristics in vitro provided appropriate passaging rate and culture condition.

从肌SC稳定的生物学特性看，可以将其作为研究肌肉受损后再生修复的细胞模型，用于检测和研究某些基因和生长因子对肌肉再生和功能恢复的影响。

The cells began aging when passaging to the eighth generation.

传至8代卫星细胞出现老化现象。

Percoll and anchoring methods were appropriate for the isolation of MSCs and 10% FBS was more suitable for the culture of MSCs. Early passaging did not affect the proliferation of MSCs.

密度梯度离心法结合贴壁分离法适于大鼠MSCs的分离；10%的FBS适合MSCs的培养；早期传代对MSCs的增殖无影响。

1 MSC derived from rabbit bone marrow can establish expanding-culture in vitro, and no change was observed in either morphology or characteristic after passaging and recovery.

兔骨髓MSC经BME诱导可表达nestin和NSE；诱导后的MSC在神经培养基继续培养，存活且分子标志的表达无增减。3。

Results The expression of Vimentin declined gradually during passaging process from the primary generation to the fifth generation, while the highest level of V...

结果 Vimentin在原贴壁基质细胞系由原代传至 5代的细胞中，其表达逐渐下降，而在再贴壁基质细胞传至第 3代中表达最高；Desmin、α-SmoothMuscleActin单克隆抗体和VonWillebrandFactor抗体染色呈阴性。

The expression of ZUB1 antigen had no difference after passaging,but it was significant decreased after differentiated into adipocytes and osteocytes,which indicated that the ZUB1 antigen may be an important marker of "stem" and was associated with the differentiation of MSCs.

当诱导骨髓MSCs向成骨细胞和脂肪细胞定向分化时，流式细胞检测显示ZUB1抗原表达相应减弱，提示ZUB1抗原可作为MSCs干细胞特征的标志分子，且可能参与了MSCs分化的细胞活动。

ABSTRACT:Nine proteins encoded by Mycobacterium tuberculosis RD1 region are important protective antigens that become absent in long passaging of Mycobacterium tuberculosis. They only exist in pathogenic Mycobacteria and are absent in Bacille Calmette-Guerin and environmental Mycobacteria. With good immunogenicities, they may play an important role in the diagnosis and prevention of Mycobacterium tuberculosis.

RD1区编码的9个蛋白，是结核分枝杆菌在长期传代过程中丢失的重要保护性抗原，仅存在于致病性分枝杆菌中，在卡介苗及环境分枝杆菌中缺失；其有较强的免疫原性，在结核病的诊断中发挥重要作用。

In serial passaging experiments, the genome of the chimeric pHBIV-1 could not exist stably. The gag or vif mRNA and the activated proteins could not be detected.

2在感染传代细胞中，嵌合克隆pHBIV-1基因组片段不能稳定存在，不能转录出gag mRNA及vif mRNA，也未能检测到有活性的蛋白。

In serial passaging experiments, the genome of the chimeric pHBIV-2 could exist stably. The transcription was active and the activated proteins could be detected.

3在感染传代细胞中，嵌合克隆pHBIV-2基因片段可长期稳定地存在，转录始终处于活跃的状态；蛋白水平亦可检测到有功能性的蛋白；电镜结果也证实了培养上清中存在类似于HIV样的病毒颗粒。

Plenty and purified satellite cells are obtained with these methods.IGF-1 could promote the proliferation and differentiation of mouse skeletal muscle satellite cell. Adequate single satellite cell could be obtained by frequent passaging method and the preparation are ready for for satellite cell transplanting.

结果：此方法可以获得足量、纯度较高的肌卫星细胞，适宜浓度的IGF对肌卫星细胞有较强的促增殖及促分化作用，频繁传代可以获取足量的单个肌卫星细胞，为肌卫星细胞移植做好了前期准备。

The French are not suggesting a new EU treaty, but they have plenty of other wheezes.

法国人并不是在建议签署一个新的欧盟条约，但他们有许多其他的阴谋。

This letter was written by my brother.

这封信是我弟弟写的。