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In cells that were positive for K3, weakly positive p63 staining was observed. After airlifting, involucrin showed positive staining in cells on the upper part of the cell sheet while staining for connexin 43 was observed in the basal layer.

暴露于气液面后，培养的角膜缘上皮细胞形成3-5层结构，外皮蛋白在外层细胞表达阳性，连接蛋白43在基底层的细胞中表达，而K3阳性的细胞很稀疏。

According to the energy conservation theory, BOM and CSIM4 were coupled. The BOM has no treatment on transmission solar radiation, which is of great importance when the model is adapted to Arctic Ocean. So the treatment was introduced to BOM. Through numerical test on different lead albedos, it was found that sea ice thickness is not so sensitive to lead albedo, which may be contribute to the lead occupies little ratio within multiyear sea ice pack. The reason of summer over-melt of arctic sea ice is the NCEP reanalysis downward solar radiation being larger than its reality. Then the arctic sea ice climate variability was simulated. Results showed that: simulated ice thickness change is in accord with the submarine investigated mean sea-ice draft changes. Simulated annually maximum ice thickness along the Eurasian continental oceans are closely related to the observed ones. The long-term mean simulated ice motion has the same features of the SSM/I derived ice motion. Sea ice extents in differential sub-regions have same trends comparing to the satellite passive-microwave data derived ones. Simulated ice concentration is closely related to the observed in the Arctic sub-regions. Sea ice flux through the Fram Strait involves ice concentration, motion and thickness. It is a composite criterion for sea ice model evaluation. The simulated ice area and volume export through the strait accord with the satellite derived or statistically reconstructed ones.(5) The simulated ice thickness climate variability and mean sea surface current of the coupled model were analyzed, results showed: the total ice volume in the Arctic Ocean has a significant decreasing trend. The volume variability is of a 10-year timescale oscillation, with two major periods of 12-13a and 18-20a. Mean ice thickness in the arctic sub-seas has different tendencies. It has an increasing trend in the Barents-Kara Sea and Baffin Bay-Labrador Sea, and decreasing in the others. The characteristic time scale of 7-10a wherein the river discharges leads the Fram Strait ice volume export is about the period that river water takes to be conveyed across the Arctic Ocean.(6) Using the simulated ice distribution in the Arctic Ocean and China precipitation, air temperature and SST in tropical key regions, the climate teleconnection were studied. Result showed: When the mean sea ice thickness is large in the central Arctic Ocean and Chukchi-Beaufort Sea , and small in the Barents-Kara Sea and Baffin Bay-Labrador Sea , the precipitation in South China, Tibetan Plateau, and the north part of Northeastern China are always smaller than normal, and v. v. When the mean ice thickness is small in CA, BC, East Siberian Sea and Greenland-Iceland-Norwegian Sea , and large in BL, The air temperature in north-eastern China, the southern of Tibetan Plateau, and Hainan Island, are always lower than normal, and v. v. In addition, when the sea ice is thick in BC and BL, the SST is larger in the middle and eastern Pacific Ocean, and is smaller in the tropical Southeastern Indian Ocean.

由于BOM没有考虑透射太阳辐射的物理过程，研究表明透射太阳辐射对北冰洋的能量收支起到重要作用，因此在BOM模式中引入了对透射太阳辐射的处理；通过对不同水道反照率的数值试验表明海冰厚度对水道反照率的敏感性不强，可能与海冰区水道面积占的比率很小有关；而模式模拟的北极海冰夏季&过度融化&主要源于NCEP再分析资料提供了偏大的太阳短波辐射；对北极海冰的气候变率进行了模拟研究，结果表明：模拟的海冰厚度变化与潜艇探测的海冰吃深度变化具有一致性；模拟和观测的亚欧大陆沿海的年内最大海冰厚度有很好的相关；模拟的海冰移速与长期平均的卫星反演的海冰移速具有相同的速度分布特征；模拟的各个海区海冰面积的变化趋势与卫星反演资料分析的结果基本一致；模拟与观测的主要海洋分区的海冰密集度具有很好的相关：弗瑞姆海峡的海冰体积和面积的输送涉及到海冰密集度、厚度和移动速度，是判断模式模拟能力的一个综合的指标，模式模拟的结果与卫星反演或重建的面积输送、体积输送具有很好的一致性；(5)分析了模拟的北极海冰厚度的气候变率及气候平均表层海流场，结果表明：北极海冰的总体积有显著减少的趋势，北极海冰总体积的变化具有10a际尺度振荡的特点，存在18-20a和12-13a两个主周期；北极海冰的平均厚度在各个海区的变化趋势不同，在巴伦支—喀拉海和巴芬湾—拉布拉多海地区海冰厚度有显著的增加趋势，而其它海区存在减少的趋势；通过对模拟的气候平均表层海流的分析表明，北极河流流量超前弗瑞姆海峡海冰流量7-10年的特征时间尺度与表层海流的气候分布存在着必然联系：(6)利用模拟结果以及中国降水、气温和热带关键区SST资料，讨论了北极各海区海冰平均厚度与中国降水、气温以及热带关键区SST的关系，结果表明：在北极中心海区和楚科奇—波弗特海海冰厚度偏大，在巴伦支—喀拉海以及巴芬湾—拉布拉多海海冰厚度偏小，则中国降水在华南地区、青藏高原和东北北部降水偏少，反之相反；在北极中心海区、东西伯利亚海、楚科奇—波弗特海以及格陵兰海海冰厚度偏小，在巴芬湾—拉布拉多海海冰厚度偏大，则在中国东北地区、高原南部地区和海南岛附近气温偏低，反之相反；另外，北极楚科奇—波弗特海和巴芬湾—拉布拉多海海冰厚度偏大时，在热带中东太平洋海温偏高，而在热带东南印度洋海温偏低。

The cell cycle and apoptosis and the expression of P-pg, bcl-2, P53 in the MG63 and MG63/R cell lines were analyzed by flow cytometry. Results The resistance index of the MG63/R cells to cisplatin was 83.557±4.841. The cells also had resistance to doxorubicin, vincristin, methotrexate and cyclophosphamide. Disordered structure of the MG63/R cells was observed through microscopy. The cells appeared in triangle, polygon and polynucleation. The increase of granular endoplasmic reticulums and apophyses was observed through transmission electron microscopy. The proliferation ability of MG63/R increased significantly, with a low apoptosis index.

结果 经顺铂186d的诱导，建立了MG63/R，其对顺铂的耐药指数为83.557±4.841，对阿霉素、长春新碱、氨甲基蝶吟、环磷酰氨亦产生不同程度的交叉耐药；光镜观察可见MG63/R细胞排列不规则，形态呈三角形、多角形及多核现象；透射电镜显示MG63/R细胞表面突起增加，粗面内质网丰富；细胞周期分析显示细胞增殖能力明显增加而细胞凋亡明显降低；MG63/R细胞 P-pg、bcl-2阳性表达较MG63细胞明显增加，P53表达则明显降低。

Then the animals were sacrificed and the surface preparation and the section of cochleae stained by MyosinⅥwere observed.Other cochlea were observed with SEM.

分别于术后第3.5个月分别行听性脑干反应检查后取双侧耳蜗标本做基底膜铺片和耳蜗冰冻切片MyosinⅥ染色观察有无新生毛细胞样细胞出现，部分耳蜗作扫描电镜观察。

The results show that the mechanical properties of this PMMA depend strongly on the testing temperature. The Young's modulus and flow stress were found to decrease with increasing temperature at low strain rate. At the strain rate of 10^(-1) 1/s, strain soften was observed under all experiment temperatures. At high strain rate, with the temperature increase, the flow stress deceases remarkably while the failure strain increases, and the strain softening was also observed at the temperature above 333K.

试验结果表明：在准静态载荷下，随着温度的升高，材料的弹性模量和流动应力减小，在应变率为10^(-1) 1/s时表现出明显的应变软化行为；在高应变率(550 1/s)下，随着温度的升高，材料的流动应力逐渐减小而破坏应变增大，当温度超过333K时也有应变软化现象发生；在相同温度下，随着应变率的升高，材料的流动应力增大，但破坏应变减小。

The surface and the cross-section of the alloy films before and after hydrogenation were observed by scanning electron microscope and atomic force microscope, which showed that the lower reflection observed in the dehydrogenated film is due to the rough surface originating from the first hydrogenation.

利用扫描电子显微镜和原子力显微镜观察了吸放氢前后薄膜的表面及剖面形貌。

RESULTS Degra-dation of PCL was the slowest one.Mass-loss was not obvi-ous:molecular mass of pancreatic suspension lost by19.8%and no outstanding physical change was observed.PCL2/1loses mass by0.5%in24wk,and molecular mass loses by61.7%with etches on cuttings sections.PCLA1/2loses mass by40.4in24wk,molecular mass loses by6.6%,and cracks and holes were observed as the main physical charac-ter.PLA lost mass by79.2%in24wk,molecular mass lost by15.1%and this material was totally degraded.

结果 PCL在24wk内各种性质变化最慢：质量损失不明显，分子质量在胰腺悬液中下降19.8%，形态无明显变化；PCLA2/1在24wk时质量损失0.5%，分子质量下降61.7%，材料断面出现蚀刻形态；PCLA1/2在24wk质量损失40.4%，分子质量下降6.6%，形态出现空洞、碎裂；PLA在24wk质量损失79.2%，分子质量下降15.1%，形态完全降解。

The effects of stimulation with electro-acupuncture on the pain threshold, 5-HT in brain stem, the β-END in hypothalamus and hypophysis, the β-END and ACTH in the blood plasma as well as the function of the cellular immunity of the normal rats, the hypophysis extirpated rats and the PHA injected rats were observed in this experiment. In the meantime, the pain threshold of rats injected with cyclophosphamide was observed too.

本实验观察了电针对正常、摘除垂体以及PHA大鼠痛阈、脑干内5—HT、下丘脑、垂体内β—END和外周血浆中β—END、ACTH含量以及机体细胞免疫功能的影响；同时，还观察了电针对Cy大鼠痛阈的影响。

Methods SD rats were used to make hemorrhagic shock model by 45% hemorrhage. Effects of Lactated Ringer's, hydroxyethyl starch alone or combined use with different volume on blood gas were observed. Meanwhile, the survival time was also observed.

SD大鼠放血45%复制重度失血性休克模型，分别观察不同量的乳酸林格氏液，羟乙基淀粉单用与合用对失血性休克大鼠血气指标的影响，同时观察动物的存活时间。

Results There was a similar distributive pattern of Neul, PPCA and β-gal in the inner ear. Neul intense staining was observed in the cochlear spiral ganglion cells, spiral limbus, spiral ligament, vestibular ganglion cells, cristae, maculae hair cells, and weak staining in inner hair cells, outer hair cells, supplying cells of the organ of Corti and stria vascularis. The intense staining of PPCA and β-gal were observed in the spiral ganglion and vestibular ganglion cells, and weak staining in the spiral limbus, spiral ligament, stria vascularis and organ of Corti. The inner ear exhibited no staining when Neul, PPCA and β-gal were deficient, respectively.

Neul最强的染色主要在螺旋神经节细胞、螺旋韧带、螺旋缘、前庭神经节细胞及壶腹嵴、球囊和椭园囊感觉毛细胞，较弱的染色分布于血管纹和Corti器内、外毛细胞及支持细胞；PPCA和β-gal在螺旋神经节和前庭神经节细胞有较强的染色，血管纹、螺旋韧带、螺旋缘和Corti器内、外毛细胞及支持细胞呈较弱的染色反应；各自酶缺乏时内耳免疫染色消失。

It was just a normal day of school

那只是上学的普通一天

Which is a very good reference in this issue.

这是一个在这个问题上很好的参考。