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Methods: The traditional method of chronic serum sickness was modified by adding followings: one kidney removed, intraperitoneal inject LPS etc., and the effect of Shenhua Tablet on 24h proteinuria, blood biochemical indices, kidney function, pathology and immunofluorescence changes was observed compared with Fosinopril, and and the effect of Shenhua Tablet on the ED-1 positive cell number in the glomerulus, protein and mRNA expression and activation of Stat3 and protein and mRNA expression of TIMP-1 in the kidney tissue, and a-SMA positive mesangial cells were observed compared with Fosinopril using western blot, northern blot and immunohistochemistry methods.

对传统的大鼠慢性血清病肾炎模型制作方法进行以下改良：切除大鼠一侧肾脏、腹腔注射大肠杆菌内毒素、以及改尾静脉注射为腹腔注射。与西药福辛普利作对照，观察中药肾华对改良慢性血清病肾炎大鼠尿蛋白、血生化、肾功能、肾脏病理变化的影响。并通过免疫组化、Western蛋白印迹及Northern杂交方法，观察中药肾华对改良慢性血清病肾炎大鼠肾组织肾小球内炎细胞浸润、Stat3蛋白与基因表达及活化、以及其下游调控的TIMP-1蛋白与基因表达、系膜细胞增殖的影响。

The SMMC-7721 hepatoma cell line of experimental groups and control groups were cultured with the addition of sodium selenite, potassium iodide or triiodothyronine (T3). The cell quantity was checked by the MTT assay , and the cell cycle was observed by flow cytometry and levels of AFP and ALB in the culture solution were determined by RIA.The SD rats were divided into positive control group, negative control group, T3 group and mixture group (the mixture of sodium selenite, KI and T3). After two weeks of the administration of sodium selenite、 KI and T3,Walker256 cells were transplanted into the abdominal cavity. The administration was kept on for another two weeks, then observed the morbility and mortality of the rats with ascites carcinoma. At the end, the ascites were for the cells counting and the cell cycles analysising.

方 法：在体外，应用细胞培养的方法，培养SMMC－7721肝癌细胞系，分为试验组和对照组，试验组加入不同浓度Se、I或T3干预，培养一段时间后，通过噻唑蓝试验、流式细胞仪，观察细胞数量及细胞周期的变化，测定细胞培养上清液中甲胎蛋白、白蛋白的水平，观察细胞分化情况；在体内，将试验用SD大鼠分成阴性对照组、阳性对照组、T3组和混合组（为Se、I和T3混合物），首先预防性用药2周，然后给试验组大鼠和阳性对照组大鼠腹腔注射Walker256细胞，制造腹水癌大鼠模型；继续用药2周，观察各组大鼠发病率及死亡率，测量不同时期大鼠血清FT3含量，通过流式细胞仪测量大鼠腹水细胞数及细胞周期变化。

Methods: The shape and formation of superior lumbar triangle were observed from the external surface of posterior abdominal wall. The grouping patterns and courses of subcostal nerve, iliohypogastric nerve and ilioinguinal nerve passing the front of superior lumbar triangle were also observed.

从腹后壁外面观察腰上三角的形态及构成，由其内面观察经过该三角前方的肋下神经、髂腹下神经和髂腹股沟神经的组合形式及走行，并分别测量其与第12肋下缘的距离。

Only small numbers of shrunken cells were observed in Cyclin B1 over-expressing cells, while markedly morphological changes were observed in the control subline.

Cyclin B1蛋白高表达抑制细胞凋亡的可能机制是，正常表达的Cyclin B1蛋白存在于细胞质中，促进细胞增殖、抑制细胞凋亡；药物诱导的Cyclin B1蛋白存在于细胞核中，促进细胞凋亡。

A marked accumulation of cells in G2/M phase was observed in CNE-2R cells during the period from 12 to 36 h after irradiation A little increase of cells in G2/M phase was observed in CNE-2 cells from 12 to 24 h after irradiation. CONCLUSION: The new subline CNE-2R is more radioresistant comparable to its parental CNE-2R cell line, and has a different cell cycle distribution.

人鼻咽癌细胞株CNE-2经间歇性大剂量γ射线多次照射后得到的CNE-2R具有稳定放射抗拒性，并且显示出与亲代不同的细胞周期特征。

Methods］ MTT assay was employed to test the lethal concentration 50 (IC50) of sensitive germline (SK-OV-3) and ovarian cancer cell subline induced by ADR (SK-OV-3/adr) treated by 4 drugs, resistance index and reverse multiple were calculated. The changes of drug content were observed by fluorescence microscope, the intracellular accumulation of ADR was evaluated by fluorospectrophotometry, the changes of mdr 1/P-gp resistance were observed respectively by RT-PCR and Western blot.

方法］MTT法检测药物对敏感细胞株（SK-OV-3）和诱导的耐药细胞株（SK-OV-3/adr）的半数致死浓度（IC50），计算耐药指数和加逆转剂后的逆转倍数，荧光显微镜观察不同时间细胞内的药物含量变化，荧光分光光度法测定细胞内ADR的含量，RT-PCR和Western blot法检测细胞耐药前后mdr1/P-gp的变化。

Methods A pig ischemic model was established by cross-clamping of the abdominal aorta above the celiac artery for 1 hour, and aortic bypass was used at the same time. The survival rate and complications of the animals were observed after operation. Pathological changes of the spinal cord were also observed on month after operation. Primary study on the reconstruction of suprarenal abdominal aorta of pig with aortic bypass was done.

建立在小猪腹腔动脉开口以上阻断腹主动脉1小时和辅助主动脉转流的模型，观察术后动物出现并发症及组织病理学的改变，研究辅助主动脉转流重建肾上腹主动脉的效果。

Thrombocytopenia was observed in another case. Anemia and fever were both observed in 2 cases. In conclusion.

在另一名患者中亦出现了血小板减少。2名患者出现了贫血和发热。

Thin leaf section were prepared from fresh tissues of YS-lnfected and healthy citrus plants, when observed under a fluorescence microscope (Type, Olympus AH-FL-2), a yellow fluorescence was distinctly observed in the YS-infected phloem tissues and not in the phloem tissuses of healthy plants and of those infected with tristeza, exocortis, canker, scab, greasy...

将健叶和黄龙病叶叶柄用保险刀片徒手切片，在透射式荧光显微镜下检查，可看到健叶叶柄的木质部导管细胞壁发黄色荧光，韧皮纤维细胞发绿色荧光，而病叶叶柄韧皮部中有1～多个鲜明的黄色或黄绿色荧光团块。这种特异性的荧光，在健叶柄韧皮部中是没有的，其它由病毒、类病毒、细菌、真菌等病原引起的病叶柄韧皮部中亦没有此种特异性荧光。

The samples were smeared on slides and stained with Giemsas stain. The stained smears were observed microscopically and photographed. It showed that except typical trophozoites in binary fission. the following trophozoites of abnormal morphs were also observed. for instance abnormal trophozoites with binary fission; enlarged trophozoites with round and irregular shaped eosinophilic bodies in the cell plasma; enlarged cells containing round and irregular shaped eosinophilic bodies and flagella; enlarged cells which contain 6 or 8 nuclear-like bodies and flagella, 3 or 4 embryonic forms of daughter trophozoite in one mother trophozoite; 4 trophozoite embryonic forms with fused plasma 3 trophozoites with fused plasma;4 trophozoites with fused plasma; a pair of trophozoite with binary fission fused with another trophozoite;2 pairs of trophozoite with binary fission fused each other; a trophozoite with 1 nuclear only.

结果 除观察到典型的营二分裂法繁殖的贾第虫滋养体外，还可见到多种形态异常的虫体，包括；虫体呈非典型二分裂；滋养体胀大，胞质中有团块状和不规则形状的嗜酸性物质；在胀大的贾第虫细胞内含有团块状和不规则形状的嗜酸性物质以及鞭毛；在胀大的贾第虫细胞内含有6或8个核状物以及鞭毛；在一个母体细胞中含有3或4个子体细胞的雏形；胞质互相融合的4个滋养体的雏形；胞质互相触合的3个滋养体，胞质互相融合的4个滋养体；1对营二分裂的滋养体与另一个滋养休互相融合；2对营二分裂的滋养体互相融合；仅有1个细胞核的滋养体。

A historical region of southwest France between the Pyrenees and the Garonne River. The duchy of Aquitaine was joined with France after the marriage of Eleanor of Aquitaine to King Louis VII in1137, but its possession was disputed after her subsequent marriage to Henry II of England.

阿基坦法国西南部一个历史地区，位于比利牛斯山和加隆河之间，在1137年阿基坦的埃莉诺与国王路易斯七世结婚之后阿基坦公国加入法国，但当她再嫁英国亨利二世后，其归属权便受到争议

There are moments when I look at the smiling , happy faces of my expectant staff and wonder how they will cope when confronted by an outraged housemaster along with an unrepentant and recalcitrant bleached blond teenager in 18 years ' time .

有时候，我看着那些满怀期待的员工微笑，幸福的脸，想着当他们遇到一位愤怒的宿舍管理员和一位不知悔改，叛逆，而且将头发染成白色的18岁少年时，他们会如何处理。