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BMSCs were isolated from adult rats by modified bone marrow culture method. BMSCs were inoculated in basic medium containing 10%fetal bovine serum, high glucose DMEM, 100 U/L penicillin, 100 U/L streptomycin and 2 mmol/L glutamine in the control group. BMSCs were inoculated in conditioned medium (basic medium, 10 mmol/L β-glycerophosphoric sodium, 10-7 mol/L dexamethasone, 50 mg/L vitamin C).

通过改良的骨髓培养法分离成年大鼠骨髓间充质干细胞,对照组以基础培养液(10%胎牛血清+高糖DMEM+100U/L青霉素+100 U/L链霉素+2 mmol/L谷氨酰胺)培养,实验组用条件培养液(基础培养液,10 mmol/Lβ-甘油磷酸纳,10-7 mol/L地塞米松,50 mg/L维生素C)进行诱导培养。

Degradation test in vitro was carried out in phosphate buffer solution (0.1M, pH7. 4) at 37 ℃. The buffer solution was changed daily. Degradation test in vivo was implanted the sample to subdermal in adult ICH rat in the scapular area lateral to the dorsal midline. At suitable time the samples were recovered. Molecular weight changes in surface layer and bulk of polymer sample were measured by GPC and weight loss was determined gravimetrically. It was found that the degradation behavior can be regulated by changing the composition of copolymers. The critical compositions from surface to bulk degradation behavior for PGCA, PLCA, PLMCA, PLDCA systems were 15-20, 20-30, 30- 40, 40 of mol percent GA or LA unit in copolymers, respectively. The degradation behavior of PGCA, PLCA, PLMCA, PLDCA systems were compared and analysed. Some factors influencing the degradation character, such as copolymer composition, hydrophobicity, crystallinity and enzyme affect etc. played important role.

体外实验中材料降解环境为37℃,0.1M,pH7.4磷酸缓冲溶液,每天换液,定期取样;体内实验中将聚合物试样埋置于ICH小白鼠背部肩胛骨皮下部位,定期处死小鼠,取样,将体内体外样品进行重量损失及试样内外层分子量变化测定,分析各聚合物试样降解行为特性,实验结果证明,改变共聚物组成,可以调节各聚合体系降解行为特性,对PGCA,PLCA,PLMCA,PLDCA共聚体系,交酯摩尔百分含量15-20%,20-30%,30-40%,40%分别为各体系内降解行为特性由表面降解型向本体降解型过渡的临界转折点,交酯含量较低的聚合物不同程度地表现表面降解行为特性,论文对各共聚体系体内外降解行为作了分析对照,例如共聚物组成对材料降解速度与降解行为的影响;生物体内酶对降解行为的影响;材料亲疏水性,聚合物链段结晶行为及碳酸酯结构对材料降解行为的影响等,得出交酯/环碳酸酯共聚体系降解行为一些共性和规律。

The activation energy for crystal growth,Ec=190.6kJ.mol-1, and the kinetic parameter m=3 were evaluated by DTA and kinetic method. The main crystal phase of glass ceramic is hedenbergite and has good strength property.

结果表明,当TiO2含量小于10%时仅产生表面晶化,而当TiO2含量为10%时可发生整体晶化,其活化能为190.6kJ.mol-1,动力学参数m=3,晶体从晶核界面呈三维生长。

In 0.015 mol/L NaOH medium, AuCl4(superscript -)reacted with hexadecyl trimethyl ammonium bromide, forming water soluble CTMAB-AuCl4 complex that exhibited a weak absorption peak at 400 nm. In the presence of hydrazine, the reaction produced orange-red nanogold particle and exhibited a surface plasma resonance absorption peak at 520 nm.

在0.015mol/LNaOH介质中,氯金酸根离子AuCl(上标-下标 4与阳离子表面活性剂十六烷基三甲基溴化铵反应生成水溶性的CTMAB-AuCl4缔合物,在400nm处产生一个很弱的吸收峰,当有肼存在时,反应生成橙红色的金纳米粒子,在520nm处出现一个表面等离子体共振吸收峰,从而可实现废水中微量肼的分光光度测定。

The temperature and the addition of surfactant can accelerate conversion and selectivity of reaction. Under the conditions of 200℃、0.38MPa、0.06 mol/L、t=4h,the conversion of hexene is 86.32%.Selectivity of 2-hexene and 3-hexene is 30.46% and 57.86%.

结果表明,当表面活性剂的加入以及反应温度的升高,能明显提高反应的转化率和选择性,在200℃、0.38MPa、0.032 mol/L的反应条件下,反应4小时底物转化率为86.32%,3-己烯的选择性为57.86%,2-己烯的选择性为30.46%。

MB (10 μ mol/L), an inhibitor of GC, not only blocked completely the relaxation of HIMA, but also enhanced the vascular contraction induced by norepinephrine.

MB是GC的抑制剂, 10 μ mol/L的MB不但使VNP舒张HIMA的作用完全消失,而且可增强HIMA对去甲肾上腺素产生的收缩反应。

This effect was observed clearly at final concentration between 1 and 10μ mol/L after treating for 24 hours. We concluded that the possible mechanisms of hydroquinone bleaching hyperpigmented skin were through its inhibition of tyrosinase activity at lower concentration and its cytotoxicity at higher one.

提示18α-甘草酸双胺盐、熊果苷的皮肤脱色作用机制与氢醌可能不同,后者低浓度时以抑制酪酸酶活性为主,高浓度时主要是细胞毒,且细胞早期的损伤部位可能发生在树状突。18α-甘草酸双胺盐能否成为一种无细胞毒酪氨酸酶抑制型皮肤脱色剂有待进一步临床加以验证。

ObjectiveThe study was performed to pretreat with TP and Vit - C, respectively and then injected 25 mol/kg CdCl2 hypodermically. Liver and kidney index , serum activities of GPT and LDH, cadmium level and GSH,MDA concentration, GSH - Px activity in the liver, and renal cortex for the animals were determined 24 hours after the administration of cadmium .

目的 本文通过预先投与TP和Vit-C后对大鼠进行急性染镉,24h后测定其肝脏和肾脏的脏器系数,血清中的GPT和LDH活性,肝脏和肾脏皮质的Cd含量,GSH和MDA含量以及GSH-Px活性,观察TP和Vit-C预处理对急性染镉所致的肝脏和肾脏损伤的影响。

At 10^(-9)mol/L of the immunotoxin, 3 logs (99.9%) of target cells were killed, but no cytotoxicity on nontarget cells.

在1×10^(-9)mol/L浓度时对靶细胞的杀伤可达到99.9%,而对非靶细胞则无细胞毒作用。

The results indicated that excellent yields of bis(indol-3-yl)methanes were obtained when indoles reacted with aldehydes and cyclo-ketones catalyzed by 20 mol% DBSA in water.

结果表明:在20mol%的DBSA的催化作用下,吲哚与芳香醛、脂肪醛和脂环酮高产率得到相应的双吲哚衍生物。

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