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Observation was done at 3, 24, 72, and 120 hours after injection using light microscopy, fluorescent microscopy, and confocal microscopy.

在注射3，24，72，120小时后，分别用光学显微镜、荧光显微镜、共聚焦显微镜观察实验结果。

In this work,atomic force microscopy,near-field scanning optical microscopy,laser scanning confocal microscopy and quantum dot labeling were used to investigate the biophysical properties(the parameters including morphology,membrane nanostructures,membrane pore, friction distribution,adhesion properties,specific interaction of antigen-antibody,distribution mode of membrane receptors and so on) of T lymphocytes stimulated by phorbol dibutyrate plus ionomycin at nanoscale.

本论文基于AFM和NSOM，结合量子点标记及激光共聚焦等技术，以人外周血T淋巴细胞为研究对象，在纳米尺度研究了活化前后以及不同活化阶段T细胞生物物理特性的变化，即细胞形态结构、膜表面纳米结构、膜孔变化、膜表面摩擦力分布、膜表面粘附特性、抗原-抗体间特异性相互作用力、膜表面受体分子分布模式等。

The ontogeny, phylogeny and ultrastruct of the glandular hairs of 5 varieties and 38 species in 26 genera of Labiatae are studied with the light microscopy, the scanning electron microscopy and the transmission electron microscopy in the paper.

本文利用光学显微镜、扫描电子显微镜和透射电子显微镜对唇形科26属38种和5变种的腺毛进行了个体发育、系统发育和超微结构的研究。

The 28 species of Cycas debaoensis, Stangeria eriopus and Macrozamia moorei etc.in 3 families of Cycadopsida were carried out anatomical studies on vascular tissue, the roots ,stems and leaves of these materials were made histological section by paraffin method and freeze method ,light microscopy observation and the dissociated materials of every organ for light microscopy and scanning electron microscopy observation research.

本探究对苏铁纲3个科的德保苏铁、托叶铁、摩瑞大泽米等28个苏铁种类进行了维管组织的解剖学研究，其中对其根、茎、叶进行石蜡切片和冰冻切片观察研究；对各器官材料进行组织离析，光学显微镜和扫描电子显微镜观察研究。

Surface waxes of the pericarp were observed by scanning electron microscopy; the structure and composition of cell wall were studied using light microscopy, transmission electron microscopy and Fourier transform infrared microspectroscopy respectively; the changes of calcium in the cell wall were determined by atomic absorption and the integrity of cell membrane by conductivity meter respectively.

本论文采用扫描电子显微镜研究果实表皮蜡层的变化，用光学显微镜、透射电子显微镜和傅里叶变换红外光谱仪研究果实细胞壁结构和成分的变化，用原子吸收分光光度计测定细胞壁钙离子的变化，用电导率仪检测果实细胞的完整性。同时测定了果实酚类物质含量、多酚氧化酶(EC 1.10.3.1)活性、过氧化物酶(EC 1.11.1.7)活性，并分析了果实硬度、糖和酸含量等品质指标。

Examination the cell shape and pseudopodia with confocal laser scanning microscopy, scanning electron microscopy, and atomic force microscopy, we found that cell spreading well on nanogrooved substrates and their body was more flat and slim; meanwhile, on plain substrates, cells were mostly round.

纳米沟槽能很好地促进细胞贴壁黏附、铺展、生长和增殖，并影响细胞的周期；纳米沟槽诱导细胞骨架发生重组，肌动蛋白骨架沿着纳米沟槽的方向取向排列；纳米沟槽影响细胞伪足形成的方式和形成与细胞中的位置。

In this paper several technologies, such as Fourier transform infrared spectroscopy, elemental analyzer, wide angle X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy and high resolution transmission electron microscopy were used to systemically investigate the evolution of crystal structure, chemical structure, microstructure and properties of wet-spinning and dry-jet wet-spinning PAN precursor fibers during proxidation and carbonization process, analysis the structural change of carbon fibers during graphitization, and discuss the correlation between the structure and properties.

本文采用傅里叶变换红外光谱、元素分析仪、X射线衍射仪、场发射扫描电镜、透射电镜以及高分辨透射电镜等测试技术，分别系统研究了湿纺原丝和干喷湿纺原丝在预氧化和碳化过程中纤维晶体结构、化学结构、微观结构和力学性能的演变规律，分析了碳纤维在石墨化过程中的结构转变，并对碳纤维结构与性能的相关性问题作了探讨。

The prior studies used transmission electron microscopy and fluorescent confocal microscopy have suggested that the endocytosis of GNPs is size-dependent. In this thesis, we used dark-field optical section microscopy to study the endocytosis of GNPs with different sizes (13 nm, 45 nm, 70 nm and 110 nm) by various cells (human lung cancer cell, CL1-0 and HeLa cells).

延续此研究，本论文利用了此光学架设，探讨了不同粒径之奈米金颗粒(13、45、70及110nm)与非小型肺腺癌细胞(CL1-0)及HeLa 细胞之相互作用(胞吞或是黏附於细胞膜上)。

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞，运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化；用gp120处理并用anti-gp120-FITC进行染色，运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况；用抗磷酸化ERK 抗体免疫荧光方法进行染色，运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

Materials and Methods Thirty SD rats, feeding in clearing grade standard, were classified into five groups at random. Application of transmission electron microscopy, scanning electron microscopy, light microscopy and immunohistological staining meth- ods, normal morphology of outflow pathways was observed and component of extra cel- lular matrix was defined. The effects of 50 IU of TNF-α given intracamerally on the morphology of outflow pathways and other tissues of anterior segments, expression of trabecular stromelysin and the change of components of trabecular extra cellular matrix were observed in 24h, 3days, 7days and 14days after injection.

中文题名TNFα对鼠眼房水外流影响的形态学观察副题名外文题名 The effect of TNFα on morphology of outflow pathways in rat eyes 论文作者王大博导师王竫华教授学科专业眼科学研究领域\研究方向学位级别博士学位授予单位青岛大学学位授予日期2002 论文页码总数90页关键词青光眼房水外流通道肿瘤坏死因子-α馆藏号BSLW /2003 /R775 /1 目的在观察SD大鼠眼房水外流通道形态及确定其房水外流通道细胞外基质成份的基础上，探讨房水中肿瘤坏死因子α浓度的增加是否能诱导小梁细胞基质溶解素的表达和分泌，进而启动小梁细胞外基质的重塑过程。

The second is to cut a mere 100 calories from your diet , which is the equivalent of a tablespoon of butter.

第二个就是从你的饮食中减少一百卡路里，只是相当于一大汤匙的黄油。

This is where the Rorschach test comes in.

现在，罗氏测试就出现了。