查询词典 microscope carrier

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Results The according perception of Normocytic, Microcytic and Non-classified RBC between phase contrast microscope and UF-100 urine sediment analytic instrument RBC-info are 91.4%, 94.4%, 83.3% respectively, the according perception between phase contrast microscope and RBC-P70Fsc are 94.9%, 95.7%, 94.7% respectively, and the according perception between phase contrast microscope and RBC Fsc-DW are 84.4%, 86.8%, 90.5% respectively, the specificity of UF-100 and phase contrast microscope in glomerular hematuria and non-glomerular hematuria are 84.3%, 88.1% and 83.3%, 87.9% respectively.

结果 观察相差显微镜与流式细胞分析仪RBC-info尿红细胞均一型、变异型、混合型形态符合率分别为91.4%、94.4%、83.3%，与RBC-70Fsc符合率分别是94.9%、95.7%、94.7%，与RBC-Fsc-DW符合率分别是84.4%、86.8%、90.5%，流式细胞分析仪与相差显微镜的特异性在肾小球性血尿和非肾小球性血尿分别是84.3%、88.1%和83.3%、87.9%。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

Chapter Four, the identification of carrier in the Hamburg Rules and the Maritime Code of The People's Republic of China, puts forward some proposals for the legal solution of carrier's identification after detailed articulateness on the regimes of actual carrier and the identification of the carrier in CMC.

本章是论文的核心章节，首先简单介绍《海牙规则》的立法背景，分析《海牙规则》的适用范围及承运人的定义，分别从船舶所有人、租船人及其他人三种可能成为《海牙规则》下的承运人着手，论述了在光船租赁、定期租船、航次租船等多种情形下，如何识别承运人。

The main work can be summed up as follows: Firstly, we studied the thermal-field properties of VCSELs, and analyzed the influences of current spreading, material parameters and operating conditions on the temperature distributions. Secondly, we began with the electrode voltage and calculated the equipotential s distributions, compared the distributions of voltages and current densities in different depths of VCSELs, and then studied the influences of the oxide-confining region with different position or thickness, and the different sizes of the gain-guided aperture and emitting window on the distributions of the injected current density, carrier concentration and temperature in the active region. Thirdly, we realized the coupling of electricity, optical and thermal-fields, worked out the threshold voltage, calculated the distributions of the injected current density, carrier concentration and temperature under different offset voltages, and analyzed the impacts of temperature profile and carrier density on the refractive index, Fermi levels and optical-field. Finally, we gave the equipotential line distributions with considering N-DBR and double oxidized-confining regions, and analyzed theinfluences of N-DBR and double oxide-confining regions on the distributions of the current density, carrier concentration, temperature and optical-field.

具体工作可以概括如下：首先，研究了VCSEL的热场特性，分析了电流扩展，材料参数和工作条件对于温度分布的影响；其次，从电极电压入手，计算出激光器中的等势线分布，并对不同深度处的电压和电流分布进行比较，研究了高阻区的不同位置和不同厚度、限制层和出射窗口半径的大小对电流密度、载流子浓度和温度分布的影响；再次，实现了电、光、热耦合，求出了阈值电压，计算了不同偏置电压下的电流密度分布、载流子浓度分布和热场分布，分析了温度和载流子浓度变化对折射率、费米能级和光场的影响；最后，给出了考虑N-DBR和双氧化限制层时激光器中的等势线分布，分析了N-DBR和双氧化限制层对VCSEL电流密度、载流子浓度、温度和光场分布的影响。

Local loop transmission from the central office to the customer's premises, unbundled from local switching or other services. Local transport from the trunk side of a wireline local exchange carrier switch unbundled from switching or other services. Local switching unbundled from transport, local loop transmission, or other services. Non-discriminatory access to 911, directory assistance and operator call completion services. White pages directory listings for customers of the other carrier's telephone exchange service. Nondiscriminatory access to telephone numbers for assignment to the other carrier's telephone exchange service customers. Nondiscriminatory access to databases and associated signaling necessary for call routing and completion. Telecommunications number portability. Nondiscriminatory access to services or information to allow the requesting carrier to implement local dialing parity (the ability to complete a connection without the use of additional access codes). Reciprocal compensation arrangements.

清单的内容总的可以概括为：申请与BOC进行互连的电信公司须提供与BOC相同的话音质量；平等访问网络元件；以公正合理的速度平等接入由BOC拥有或控制的电线杆、管道、导管以及通行权；中央局至用户住宅的本地环路传输，其定价与本地交换业务或其他业务定价分开进行；从本地电话公司交换机中继端开始的本地传输与交换业务或其它业务分别定价；本地交换业务与传输业务、本地环路传输业务或其他业务分开定价；平等访问911应急电话、电话指南和话务员呼叫完成业务；享用其他电话公司电话交换服务的电话黄页；平等访问分配给其他电信公司用户的电话号码；平等访问呼叫路径和呼叫完成所需的数据库以及相关信令；电信号码便于记忆；平等访问服务或信息，使电信公司实现本地等位拨号（不需拨打任何接入码即可完成电话连接的功能）；提供相互补偿。

As under the laws and regulations of Venezuelan and the Dominican Republic ports, the Carrier is required to make delivery of the goods to the Customs after which the goods are no longer subject to the Carrier's control or disposition, the carrier's liability and responsibility in any capacity thereupon altogether ceases, and from and after such delivery to the Customs the Carrier is not liable with respect to any all claims for loss, damage, delay, non-delivery and/or misdelivery of the goods, the care, custody and proper delivery having been taken over by the Customs.

二十五、根据委内瑞拉和多米尼加共和国港口的法规，承运人应将货物提交给海关，此后，货物不再受承运人所控制或处置，承运人的责任和义务同时消除，自从交货给海关之后，承运人不负责任何有关货物灭失、损失、延误、未交货和／或错交货的索赔，照料、保管和正确交货均由海关接手处理。

The image forming device also includes a pressing body which is provided on the main frame and is slidable in a direction along a straight line orthogonal to a rotational center shaft of the electrostatic latent image carrier and a rotational center shaft of the developer carrier, and an operating unit which operates the pressing body to press a bottom surface of the developing unit by sliding the pressing body in a direction of the straight line and to hold the pressing body so that the developer carrier is maintained under a state making contact with the electrostatic latent image carrier.

该成像设备还包括：一个按压机构，该机构设置在该主框架上，并在按压方向上沿着这样直线的方向滑动，其中该直线垂直于静电潜象载体的旋转中心轴和显影剂载体的旋转中心轴；以及一个操作装置，该操作装置通过在直线的方向上滑动该按压主体以按压显影单元的底面，并保持该按压主体，从而该显影剂载体保持在与静电潜象载体接触的状态。

Beginning with three VLCCs in 1976, HMM has strengthened its competitiveness to advance new services such as bulk carrier, tramper, container carrier, LNG carrier and special product carrier.

现代商船于1976年利用仅有的3艘油轮开始运行，之后积极开拓散货船、矿砂船、集装箱船、液化天然气轮以及特殊货物船等领域，不断提高竞争力。

If any payment is made by the Carrier to the Merchant in respect of any claim arising from the combined transport carriage, the Carrier shall be automatically subrogated to or given all rights of the Merchant against all others including pre-carrier or on-carrier or Sub-contractor on account of such loss or damage.

如果承运人已就因联合运输而引起的任何索赔向货方支付任何款项，承运人便应自然而然地以代位关系而取得或被赋予货方针对所有其他人包括前一程承运人或续程承运人或分立契约人就此项灭失或损害而享有的权利。

For every one of my New Year resolutions, I have been a backslider for years.

说到我的新年决心，我每年都做

With empathy and focus on a feeling level, we can all relate to the common ground of mutuality of emotions.

有了同情心和感觉上一级的重点，我们都可以涉及到相互的情感共同点。