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Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡，原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化：细胞核固缩，核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘，并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用，与对照组相比有显著性差异(p＜0.01)，在剂量为0.8-8μmol／L范围内，抑制率随剂量的增加而增加，当剂量超过8μmol／L时，抑制率反而下降；3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性，引起DNA降解损伤，反向调节细胞周期活动，促使细胞从G0期进入G1期，抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核，可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

The morphology, number of myelinated fibers and mean thickness of myelin sheath of regenerated nerves were observed by light microscope, transmission electron microscope and scanning electron microscope.

光镜、透射电镜和扫描电镜观察再生神经的形态结构、有做神经纤维数量、平均髓鞘厚度并进行统计学分析。

Methods: Experimental mice were perfused with different dosages of tetracycline. Eighteen hours later, the serum levels of ALT, AST were measured and the liver tissues were observed by photics microscope to find the relative suitable dosage that can obviously induce acute liver injury. Then the suitable dosage was given in the same way, and the levels of ALT, SOD, MDA, GSH-PS, IL-18 in serum were measured 18, 36, 54, 72 h later respectively. The condition of cell apoptosis was analyzed, and the tissues were observed by photics microscope and electron microscope to find the relative suitable time.

先以不同剂量四环素灌胃，18h后检测小鼠血清ALT、AST水平并进行光镜观察，探索导致明显急性肝损伤的相对合适剂量；再以相对合适剂量四环素灌胃，18、36、54、72h后分别检测小鼠血清ALT、SOD、MDA、GSH-PX、IL-18水平及肝细胞凋亡情况，并进行光镜、电镜观察，探索导致明显急性肝损伤的相对合适时间及可能机制。

Of the recording media using scanning probe microscope, including scanning tunneling microscope, atomic force microscope, scanning near-field optical microscopy, etc., nanometer-scale data storge can be successfully achieved, and this technology has been considered as one of the best way to promote the storage density.

扫描探针显微技术通过改变材料的光、电、磁等局域特性可以实现纳米尺度的信息存储，成为提高信息存储密度的最有效手段之一。

The unetched specimens were instantly observed by QUANTA-400 environment scanning electron microscope,scanning tunnel microscope and optical microscope,respectively.The results show that the surface relief will be found during the bainite transforming and will be in the shape of "Λ".The formation of bainite surface relief is to ...

不经浸蚀，分别采用QUANTA-400环境扫描电镜、Nanofrist-1000扫描隧道显微镜和光学显微镜观察试样，发现，贝氏体相变过程中产生了表面浮凸，表面浮凸为&帐篷形&，并认为贝氏体表面浮凸的产生，是由于过冷奥氏体转变为贝氏体时比体积增大，且试样表面层体积膨胀不均匀造成的。

To explain relationship between the effect of CLD on senile Demention and neuron in hippocampal area. The change of natural aging mice's neuron in hippocampal area were studied by applying microscope and transilluminating electric microscope. Results: Comparing with the young control, the old control mice's hippocampus had less small pyramid cells, the nerve cells denaturalizing and their branches reducing in the microscope. Hippocampal nerve cells were degenemiceing, with nucleus membrance crinkling like wave shape were not clear, and chromatin condensing with high electron density, heterochromatin accruing, part cell organs inclosing nucleus, nucleus condensing in the EM.

为了阐明菖龙丹抗老年性痴呆作用与海马区神经元结构的关系，运用光镜和透射电镜观察了菖龙丹对自然衰老小鼠海马区形态的影响，结果显示老年对照组小鼠光镜下海马锥体细胞较青年对照组数量减少，细胞体积变小，神经元变性，分枝减少；电镜下海马神经细胞发生退化改变，核膜皱缩，呈波浪状，膜结构不清晰，核染色质浓缩，电子密度高，异染色质较多，部分胞浆细胞器向细胞核聚集，有的可见细胞核固缩。

Fig 1 At the experimental side, two weeks after operation (HE ×100) Many pieces of cartilage cells and spindle-shaped mesenchymal cells were found, by light microscope Fig 2 At the experimental side, twelve weeks after operation (HE ×100) Medullary cavity had formed, which contained hemoblasts and adipocytes, but the trabculae was not typical, by light microscope Fig 3 At the experimental side, twenty-four weeks after operation (HE ×100) The typical cancellous bone had formed, by light microscope Fig 4 At the control side, twenty-four weeks after operation (HE ×100) A large part of CXB was degraded, resorbed and replaced by fibrous tissue, but there was'not any new formed bone, by light mocroscope

图1 实验侧术后2周(HE ×100)镜下可见很多软骨细胞条索和团块生成及大量梭形间充质细胞图2 实验侧术后12周(HE ×100)镜下可见有髓腔形成，内含有造血和脂肪细胞，骨小梁尚不典型图3 实验侧术后24周(HE ×100)镜下可见形成典型的松质骨结构图4 对照侧术后24周(HE ×100)镜下可见大部分CXB被降解吸收，为纤维组织替代，无新骨形成

An optical microscope, atomic force microscope, scanning electron microscope, high resolution double crystal X-ray diffraction, and Raman shift spectrum are used to analyze the sample.

采用光学显微镜、原子力显微镜、扫描电子显微镜、高分辨率双晶X射线衍射和喇曼谱测试对薄膜进行分析。

In order to investigate the haemocyte types and the effect pearl-nucleus-inserting operation in visceral mass on haemocytes in Hyriopsis cumingii Lea, haemocyte morphology were studied by phase contrast microscope, optical microscope, transmission electron microscope and flow cytometry FCM.

为了探讨三角帆蚌血细胞的类型及内脏团插核手术刺激对血细胞形态结构和数量的影响，研究利用相差显微镜、光学显微镜、透射电子显微镜和流式细胞仪对三角帆蚌血细胞进行了形态学研究。

The cell biological features were observed by inverted phase contrast microscope, l ight microscope, electron microscope, cell vital ity assay, cell growth curve and cells staining after harvest and during the periods of culturing the primary, the 1st passage and 2nd passage.

分别在取材后、原代、第1 代、第2 代细胞培养期间，进行髓核细胞活力测定；爬片培养后进行甲苯胺蓝、HE、聚集蛋白聚糖番红O、Ⅰ型及Ⅱ型胶原免疫组织化学染色观察；MTT 法绘制髓核细胞生长曲线，并行原代及第2 代细胞透射电镜观察，对体外细胞的生物学特性进行研究。

Smoking cigarettes adds to the Computer Desk Computer manufacturer computer mouse Computer Computerized collar knitter machine Conduit Bodies construction board Container Bag Control Arm Cooking Thermometer general levels of air pollution as well as damaging our health.

吸烟不仅对健康有害，而且增加空气污染程度。香烟雾含有多种有害物质：不仅有致癌化学物质、有某种能阻止氧气进入血液的气体，还有引起血压增高、心速加快的被称为尼古丁的物质。

He is one of my favorite actors.

他是我最喜欢的男演员之一。