查询词典 microscope base

Shanghai Lingang New City, deputy director of the CMC, the Shanghai Lingang Economic Development Co., Ltd., chairman of Liu Ping, the current passenger vehicle Lingang Industrial area, the key pieces of the ship, power generation and transmission and distribution equipment, marine engineering equipment such as self-innovation-based equipment manufacturing base of the development framework has been formed basically, in the passenger vehicle manufacturing base, the SAIC independent innovation and self-brand vehicle and engine production bases have been built; The key pieces of the ship manufacturing base, the China Shipbuilding Mitsui projects, China Shipbuilding Heavy Industry Wartsila projects, Volvo times Tatsu project covers the low, middle and high-speed full range of marine engines, marine equipment, various types of enterprises started to gather support; in power generation and power transmission equipment manufacturing base, the production level of one million kilowatts nuclear power primary equipment, heavy-duty gas turbines, is extremely heavy equipment manufacturing capacity heavy electrical joint plant, as well as 1400 tons lifting capacity of 5000 tons berth conditions electrical Heavy Cargo Wharf has been completed and put into production; in the marine engineering equipment manufacturing base, the China Shipbuilding Group has started building a world-class large marine engineering and shipbuilding professionals supporting base; in the civil aviation industry support base, the China Aviation Industrial Group has signed an agreement with the city government , in the Lingang Industrial Area in Shanghai to set up an air base supporting industries.

上海临港新城管委会副主任、上海临港经济发展有限公司董事长刘家平介绍，目前临港产业区内乘用车整车、船舶关键件、发电及输配电设备、海洋工程设备等自主创新型装备制造业基地的发展框架已基本形成，在乘用车整车制造基地方面，上汽集团自主创新和自主品牌整车及发动机基地已建成投产；在船舶关键件制造基地方面，中船三井项目、中船重工瓦锡兰项目、沃尔沃遍达项目覆盖了低、中、高速全系列船用发动机，各类船用设备配套企业开始集聚；在发电及输变电设备制造基地方面，生产百万千瓦等级核电主设备、重型燃气轮机、具有极端重型装备制造能力的电气重装联合厂房，以及具有1400吨吊装能力、5000吨泊位条件的电气重件码头已建成投产；在海洋工程设备制造基地方面，中船集团已开工建设具有世界一流的大型海洋工程与船舶制造专业配套基地；在民用航空产业配套基地方面，中航工业集团已与市政府签署协议，在临港产业区设立上海民用航空产业配套基地。

Using maximum flooding surface and local explosure erosion surface as the correlation framework surface in the 4-th hierarchical base-level cycles, sand-body in the 3-th hierarchical base-level cycles formed in the 4-th hierarchical base-level cycles may be correlated layer by layer. The law of the sand-body and the characters of reservoir heterogeneity formed in the 4-th hierarchical base-level cycles are discussed. The study shows:①During early period of the 4-th hierarchical base-level cycle rising, subaqueous distributary channel formed, continuously stacked vertically, and migrated in limit range laterally, and channel sand-body stretches farther longitudinally, and lacks interbeds of mud rock and siltsand in sand rock, reservoir heterogeneity is relatively weak.②During mid-period of the 4-th hierarchical base-level cycle rising, subsaqueous distributary channel migrated actively, channel divided and converged strongly to form netted sand-body and sand sheet mainly, but interbeds of mud rock and siltsand increases gradually in sand-body, reservoir heterogeneity increased.③During later period of the 4-th hierarchical base-level rising and early period of the 4-th hierarchical base-level falling, reservoir sand-body was poor, and only form thin sand-body of subsaqueous distributary channel, subsaqeuous creveas and distal mouth bar, sand-bodys are surrounded by mud and silt rock.

进一步采用以第4层次基准面旋回中相当最大湖泛面的相转换面和具有区域性暴露侵蚀作用的层序边界面为等时地层对比框架的边界，对发育于MSC2—MSC4三个第4层次基准面旋回的第3层次基准面旋回内砂体进行了逐层等时对比，探讨第4层次等时地层对比格架内储集砂体发育分布规律，及储集砂体的非均质性特点，研究发现：①第4层次基准面上升初期发育的水下分流河道，砂体呈连续叠置、侧向迁移受限，纵向上呈长距离延伸的带状产出，砂体间泥、粉砂岩夹层少，储层非均质性相对较弱；②在第4层次基准面上升中期发育的水下分流河道侧向迁移活跃，纵向延伸过程中分流汇合作用强烈，以形成网状或席状连片砂体为主，但砂体间泥、粉砂岩夹层逐渐增多，储层非均质性增强；③第4层次基准面上升晚期和下降早期，不利于储集砂体发育，仅出现少量薄的水下分流河道、水下决口扇、远—河口砂坝砂体。

The four models were analyzed and normalized. We studied the procedure and method of building forest fire model base. Model base was build by object-oriented method based on category of model. Forest fire spreading models algorithm were implemented. We designed model dictionary base, model file base, and model knowledge base. Model base administration and maintenance functions were developed and implemented model file management, parameter management and knowledge management. The problems of interface between of model base and database were solved by means of model data control files. Model base ensured the availability of supporting for forest fire model suitability selection technology and forest fire model self-adapting updating technology.(3) Based on BP artificial neural network, we designed a frame construction of forest fire model selection of suitability. Forest fire model selection knowledge was produced through BP artificial neural network.

2建立了林火模型库建设的林火蔓延模型评价标准，根据模型评价标准和试验区实际情况，在42个林火蔓延模型中选择了目前最具有影响力和最具有典型代表性的Rothermel模型、王正非模型、加拿大模型和澳大利亚模型进行了解析和归一化处理；对林火模型库的建库过程和方法进行了研究，在林火模型分类基础上，采用面向对象的模型库表示方法建立了林火模型库；实现了林火蔓延模型算法，设计了模型字典库、模型文件库和模型知识库；开发了模型库管理与维护功能，实现了模型的文件管理、参数管理和知识管理；通过模型数据管理文件解决了模型库与数据库之间接口问题；实现了林火模型库对林火模型适宜性选择和林火模型自适应修正技术的有效支持。

The base is equipped with an X-axis workbench, a Y-axis workbench and a microscope, a bonding head is arranged above the workbench, a liftable support is arranged on the base, the microscope is mounted on the liftable support and provided with a Z-axis stepping motor, and an automatic magnification regulating mechanism is arranged on the microscope.

在底座上设置有X轴工作台、Y轴工作台和显微镜，在工作台的上方设置有键合头，在底座上还设置有可升降支架，显微镜安装在可升降支架上并且在显微镜上设置有Z轴步进电机，显微镜上安装有放大倍数自动调节机构。

Results:Whith the time and doses of ART incubation extended, ART significantly inhibited the proliferation of SGC-7901 cells and the inhibited effect shows dose-and -time-dependent. Obvious changes of apoptotic morphology were observed by invert microscope, fluorescence microscope, scanning electron microscope and transmission electron microscope, they showed that cells had marked nuclear condensation or the fragmentation of chromation as well as apoptotic , mitochondrial edema and vesicle , with the time of incubation extended , the proliferation rate become slow and the volume became small and transformed. FCM assay indicated that most of the cells were arrested in Go/Gi and the apoptotic peak appeared. During the prolong of incubation time, the apoptosis rate was increased. At the same time, the number of S and G2/M phase cells were decreased. The result of TUNEL indicant that there are apoptosis and necrosis.

结果：随着药物浓度的增加和作用时间的延长，蒿甲醚对胃癌SGC-7901细胞的抑制作用呈时间和浓度依赖关系：在倒置显微镜、荧光显微镜、扫描电镜和透射电镜下可观察到典型的凋亡细胞的形态改变，表现为：核固缩或染色质边聚或凝聚成大块状，可见凋亡小体，线粒体肿胀增殖，严重的空泡化，随作用时间的延长，细胞增殖速度减慢，细胞体积缩小变形；流式细胞术显示胃癌SGC-7901细胞出现明显的凋亡峰，随着作用时间的延长，其凋亡率逐渐升高，细胞周期阻滞在G_0/G_1期，S及G_2/M期细胞数大量减少；流式细胞术TUNEL检测结果显示细胞凋亡和坏死同时存在。

Results (1) CCD fluorescent microscope was superior to laser scanning confocal microscope in collecting the fluorescent image of DCF. CCD fluorescent microscope was chosen to be the system applied in this research with the consideration of operation, cost and et al. The mercury-arc lamp of fluorescent microscope was fit for the requirement of our study. The wavelength range of the light output was 460-490 nm and the power density was about 100mW/cm in the condition of our study. Organelle-cell fluorescence intensity ratio analysis was more suitable than other methods.

结果：(1)CCD荧光显微镜采集到的DCF荧光图像的质量优于共聚焦显微镜，同时综合考虑包括采图质量、光照射剂量的可控性、实验成本和操作简便性等诸多因素，最终确定了CCD荧光显微镜作为实验研究的工具；荧光军医进修学院硕士学位论文中文摘要显微镜的汞灯输出功率稳定，光斑均匀，经本实验所选取的光路系统后输出波长范围为460一490nm，功率密度约为1 00 mw/cm；细胞器一细胞荧光强度比值法对于荧光分布位置的确定优于其他方法。

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可程式恒温恒湿箱、高低温试验箱、盐水喷雾试验机、干燥箱、热老化试验机、耐寒/耐黄变试验箱、冷热冲击试验机、灼热丝试验机、垂直+水平燃烧试验机、漏电起痕试验机、按键寿命试验机、插头插座负载寿命试验机、弹簧试验机、90度剥离强度试验机、破裂强度试验机、环压强度试验机、纸管/纸箱抗压试验机、耐磨擦试验机、插拔力试验机、转轴寿命耐久试验机、手机翻盖寿命试验机、鼠标滚轴寿命试验机、振动试验机、模拟汽车运输振动台、手机跌落试验机、包装跌落试验机、电线曲挠试验机、插头线突拉试验机、静态拉力试验机、线材弯折摇摆试验机、线材伸长率试验机、万能材料试验机、拉力试验机、落球冲击试验机、耳机疲劳寿命试验机、耳机拉伸寿命试验机、推拉力计、布、洛、维、肖、里、邵氏硬度计、影像测量仪、精密测绘显微镜、数字投影仪、体视显微镜、金相显微镜、读数显微镜、连续变倍显微镜、放大镜、卡尺、千分尺、标准砝码、高度计、厚度计、岩石平板、电子计重称、电子计数称、电子天平、分析天平、标准光源箱等。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Results The according perception of Normocytic, Microcytic and Non-classified RBC between phase contrast microscope and UF-100 urine sediment analytic instrument RBC-info are 91.4%, 94.4%, 83.3% respectively, the according perception between phase contrast microscope and RBC-P70Fsc are 94.9%, 95.7%, 94.7% respectively, and the according perception between phase contrast microscope and RBC Fsc-DW are 84.4%, 86.8%, 90.5% respectively, the specificity of UF-100 and phase contrast microscope in glomerular hematuria and non-glomerular hematuria are 84.3%, 88.1% and 83.3%, 87.9% respectively.

结果 观察相差显微镜与流式细胞分析仪RBC-info尿红细胞均一型、变异型、混合型形态符合率分别为91.4%、94.4%、83.3%，与RBC-70Fsc符合率分别是94.9%、95.7%、94.7%，与RBC-Fsc-DW符合率分别是84.4%、86.8%、90.5%，流式细胞分析仪与相差显微镜的特异性在肾小球性血尿和非肾小球性血尿分别是84.3%、88.1%和83.3%、87.9%。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

The second is to cut a mere 100 calories from your diet , which is the equivalent of a tablespoon of butter.

第二个就是从你的饮食中减少一百卡路里，只是相当于一大汤匙的黄油。

This is where the Rorschach test comes in.

现在，罗氏测试就出现了。