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osteoblasts at 1×105/ml,2×105/ml and 5×105 /ml cell density were cultured in the DMEM medium and in the DMEM medium supplemented with β-glycerophosphate sodium and ascorbic acid.

实验选用了1×105个/ml、2×105个/ml、5×105个/ml三个不同的细胞接种浓度，并且每个浓度组分别给予含β-甘油磷酸钠和维生素C的条件培养液以及常规培养液进行细胞培养。

Objective To investigate whether osteoblasts cultured in vitro can form mineralization nodes. Method The osteoblasts at 1×105/ml,2×105/ml and 5×105 /ml cell density were cultured in the DMEM medium and in the DMEM medium supplemented with β-glycerophosphate sodium and ascorbic acid.

目的 了解体外培养成骨细胞的接量对钙化结节形成的影响方法实验选用了1×105 /ml、2×105个/ml、5×105个/ml三个不同的细胞接种浓度，并且每个浓度组分别给予含β-甘油磷酸钠和维生素C的条件培养液以及常规培养液进行细胞培养。

Methods The osteoblasts at 1×105/ml,2×105/ml and 5×105 /ml cell density were cultured in the DMEM medium and in the DMEM medium supplemented with β-glycerophosphate sodium and ascorbic acid.

实验选用了1×105个/ml、2×105个/ml、5×105个/ml三个不同的细胞接种浓度，并且每个浓度组分别给予含β-甘油磷酸钠和维生素C的条件培养液以及常规培养液进行细胞培养。

Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group compared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early compared with the DMEM medium.

结果 在培养30天内，条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成；而2×105个/ml组和1×105个/ml组都有钙化结节形成，而且有细胞越少形成结节越早，数量越的趋势；给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group cnpared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early cnpared with the DMEM medium.

结果 在培养30天内，条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成；而2×105个/ml组和1×105个/ml组都有钙化结节形成，而且有细胞越少形成结节越早，数量越多的趋势；给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

Methods: Osteogenic medium (expansion medium with 10 mmol·L -1 dexamethasone,50 μg·L -1 ascorbic acid and 10 mmol·L -1 β glycerophosphate ) was used to stimulate MSCs into osteoblast like cells.

选用生长状态良好的兔骨髓间质干细胞，在体外进行成骨活性诱导，并进行形态学观察和碱性磷酸酶、骨钙素的测定。

Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group compared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early compared with the DMEM medium.

结果 在培养30天内，条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成；而2×105个/ml组和1×105个/ml组都有钙化结节形成，而且有细胞越少形成结节越早，数量越多的趋势；给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

METHODS: The third passage of BMSCs and AMSCs at a density of 5×10^7 L^(-1) were divided into two groups. Cells in the induction group were incubated in 1.5 mL of osteoinductive medium, supplemented with 10^8 mol/L dexamethasone, 10 mol/L β-glycerophosphoric acid, and 50 mg/L vitamin C. Cells in the non-induction group were not treated with osteoinductive medium.

选取第3代骨髓间充质干细胞和脂肪间充质干细胞，按5×10^7L^(-1)接种后各分为2组：诱导组加入含10^8mol/L地塞米松、10mol/L β-甘油磷酸、50mg/L维生素C的成骨诱导培养基1.5mL，未诱导组不加入成骨诱导培养基。

Methods Peripheral blood neutrophils were islolated from health men, and then cultured in DMEM medium containing 10% fetal calf serum for 3 h. Blank medium or gonococcal inocula was added into group A or group B. NO concentration and iNOS mRNA expression at 0, 3, 8 and 12 h were determined by cadmium reduction method and realtime quantitative fluorescent PCR, respectively.

分离正常人外周血中性粒细胞，在10%胎牛血清DMEM培养液上培养3 h，A组加入空白培养基，B组加入淋球菌菌液，随后在0、3、8、12 h分别以实时定量荧光PCR测定iNOS mRNA表达，用镉还原法检测NO浓度。

We investigated the receiver functions in a horizontally-layered medium with arbitrary oriented hexagonally symmetric anisotropy, and discussed the phenomenon of shear-wave splitting and energy deviation in an anisotropic medium. The anisotropic structure yields patterns of receiver function amplitudes and arrival times on both the radial and transverse components that are a systematic function of source-receiver back azimuth. We also investigated the influence of varying anisotropy parameters, e.g.

采用具有任意方向对称轴的横向各向同性介质系统，探讨了各向异性介质中接收函数表现出的横波分裂、能量偏离等特征，并系统剖析了各向异性介质对称轴方位角、倾角以及各向异性层位分布对接收函数方位变化花样的影响。

My father is over 60 years old, and he lifts weights every day.

我爸爸六十多岁了，他每天都练习举重。

Then in a serious tone she declared:"It doesn't pay, dear boy, to look like a ninny with one's wife the first night."

两眼湿润了，她把身子蜷得很小，这样似乎可以更好地闻闻自己。