查询词典 medium computer

Methods Mononuclear cells of peripheral blood were isolated by Ficoll Hypaque centrifugation from 32 breast cancer patients and plated on 6-well culture plates(106 /ml, 2 ml/well) in RPMI1640 medium supplemented with 10％ heat-inactivated fetal bovine serum, GM-CSF, IL-4, and/or TNF-α. Two h later, nonadherent cells were gently removed and fresh medium was added.

方法采用密度梯度离心的方法，分离32例乳腺癌患者外周血中的单个核细胞，在6孔培养板上(106/ml，2ml/孔)，用含10％热灭火的小牛血清和GM-CSF、IL-4、TNF-α细胞因子的RPMI 1640培养基培养。

Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法：穿刺抽取15 mL脐带血，密度梯度法分离吸取分层界面的白色环形絮状物，其中富含单个核细胞，离心后沉积细胞用含15％胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮，调整细胞密度为1×109 L-1接种于25 T培养瓶内，放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养，72 h后更换培养基，去除红细胞及其他未贴壁细胞，至70％~80％融合时传代。

Results Cells cultured in serum-free medium AIMV could proliferate,kill target cellsand express CD11 c well enough to substitute the cells cultured in standard serum-containing medium.Proliferation,cytotoxicity and expression of CD11 c of A-NK were better than nonadherent natural killer cells.Death patterns of tumor target cells were necrosis and apoptosis.

结果 与完全培养基培养细胞相比，AIMV培养细胞增殖能力、杀伤肿瘤细胞的能力、表达CD11 c 的能力与之相当；在同一培养条件下A-NK细胞的增殖能力、杀伤活性及表达CD11 c 的能力明显强于NA-NK细胞；被A-NK细胞杀伤的肿瘤细胞死亡形式是溶解坏死和凋亡。

Results Cells cultured in serum-free medium AIMV could proliferate,kill target cellsand express CD11 c well enough to substitute the cells cultured in standard serum-containing medium.Proliferation,cytotoxicity and expression of CD11 c of A-NK were better than nonadherent natural killer cells.Death patterns of tumor target cells were necrosis and apoptosis.

结果 与完全培养基培养细胞相比，AIMV培养细胞增殖能力、杀伤肿瘤细胞的能力、表达CD11 c 的能力与之相当；在同培养条件下A-NK细胞的增殖能力、杀伤活性及表达CD11 c 的能力明显强于NA-NK细胞；被A-NK细胞杀伤的肿瘤细胞死亡形式是溶解坏死和凋亡。

CD44~+CD24~ cells in MCF-7 increased after cultured in serum-free medium. The cancer stem-like cells in MCF-7 grew as clonal nonadherent spherical clusters. The percentage of CD44~+CD24~ cells reached up to 29.23% after cultured 3 weeks in serum-free medium.

结论SP检测与CD44~+CD24~细胞检测是目前乳腺癌干细胞检测的两种常用方法，但两者用于同一种乳腺癌细胞系中的检测结果并不一致，均有一定的局限性。

Both of conventional continuous medium mechanics and noncontiguous medium mechanics, having strong pertinence, can abstract and simplify the rock model in research procedure, in which the rock body lying over the coal face can be considered as a static beam or board to leave out the push changes and the changes of property and load during the stability of overlying rock deformation has lost. Hence the derived profile equation is only the approximate expression of final subsidence of surface after the mining finished, and it is difficult to obtain the dynamic subsidence process of rock body.

传统的连续介质力学和非连续介质力学均具有针对性，在研究过程中将岩体模型抽象简化，将工作面上方的岩体看成是一静止的梁、板，省略了工作面的推进变化及覆岩在变形失稳时的性质和载荷的变化，得出的剖面方程仅是开采结束后地表的最终沉陷近似表达式，难以得到岩体的动态沉陷的过程。

Analog or nonregenerative: an amplifier inserted in a transmission medium to compensate for the attenuation and distortion introduced by the medium.

模拟的或非再生的：一种插在传输媒质中的放大器，用以补偿由媒质产生的衰减或失真。

During compilation of the , this bureau proposed plan suggestions in the respects of development idea, medium and long term goals, industrial key points, enterprise cultivation, project planning and creation of foreign environment etc., which policies and means were pertinent and operatable and displayed significant guidance to escalate non-state-owned economy in our city for medium and long term development.

我局在《重庆市非公有制经济中长期发展规划》编制过程中，提出了发展思路，中长期目标，产业重点，企业培育，项目策划和创造外部环境等方面的规划建议，对策措施具有针对性和可操作性，对进一步推动和促进我市非公有制经济中长期发展具有重要指导作用。

Adding fresh medium to cultures, when they had been cultured for 5-8 days, could promote cell sustained division, after which, adding a medium with lower concentration osmatic substance or without osmatic one could futher stimulate the cell division and ensure the active growth of the cell masses.

采用液体浅层培养方法，对埃斯基和雷蒙特红豆草的原生质体进行了培养。埃斯基花茎愈伤组织的原生质体在VK和Kao＇s培养基中2-6天分裂，2周内形成细胞团，19天时的分裂率和克隆率分别为71.41-76.19％、64.78-67.03％。

Therefore, it has the important scientific research value in studying the relationship between the changing of the parameters (including the temperature, the humidity, the grain radius and so on) and the electricity and radioactivity of the medium. Applied the count statistics and the numeral value analysis, the paper analyzes, concludes and contrasts the data sufficiently through the experimentation, and acquires the following results that the resistivity and the polarizability both have the negative relationship with the temperature variety of medium from 0℃ to 200 ℃. The parallactic poalarizability is consistent with the temperature variety in the plan as well as the section, but their trends are exactly opposite. Power supply voltage has no considerable influence to the resistivity and polarizability by using duality variance analysis, however, the detecting depth and the plan position influence them greatly. Different AB/2 interval that increases by degree does not influence variance and the average value of the resistivity and the polarizability remarkably. By using the least double multiplication, the relationship of temperature-time-the influence radius of the fire source is carried up by the mathematic drawing up. After discussing their correl ation, I educe the influence radius of the fire source and carry out the 'trend analysis and forecast to the spacial changing of the temperature and the parallactic polarizability in the end.

本文通过试验并应用数理统计和数值分析等相关理论对所得到的试验数据进行了分析、归纳和对比，得出了介质温度变化在0-200℃内视电阻率和视极化率与温度均表现为负相关性；视极化率与温度在剖面和平面上的变化形态具有很好的一致性，但变化趋势相反；由二元方差分析可得供电电压对视电阻率和视极化率无显著影响，但探测深度和平面位置对视电阻率和视极化率有显著影响；通过假设检验可证不同AB／2递增间隔，对视电阻率和视极化率的方差和平均数影响都无显著性差异；应用最小二乘法对温度——时间——火源影响半径之间的关系进行了数学拟合，讨论了它们之间的相互关系，得出了火源影响的有效半径，太原理工大学硕士研究生学位论文并对温度和视极化率的空间变化作了趋势分析和预测。

The role of the environment is very important.

的作用，环境是非常重要的。

At that time, the High Court of England and Wales Court of Queen's Bench president of William Murray, Lord Mansfield in June 22, 1772 ruling:"No matter then there is the inconvenience, but there must be a decision, I can not say that this case under the law in England are permitted or authorized; are so black should be released."

当时的英格兰及威尔士高等法院王座法庭院长William Murray， Lord Mansfield于1772年6月22日宣判：「无论有那麽不便，但总要有个决定，我不能说这件案在英格兰法律之下是准许或认可；所以黑人是应该被释放。