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The results showed that, using transient GUS expression, the frequency of resistant calli and shoot differentiation as a criteria, wheat mature embryos were cultured on the preculture medium of CM4C1P for 14 days, and immersed in inoculation suspension Ⅰ for 3 hours, then the explants were co-cultured under desiccation conditions for 3 days, which was optimal conditions for Agrobacterium-mediated transformation of wheat mature embryos.

为了进一步完善根癌农杆菌介导的小麦遗传转化体系，以小麦成熟胚为转化受体，研究了根癌农杆菌介导法转化小麦的主要影响因素，结果表明，以gus基因瞬时表达率、抗性愈伤组织获得率和分化率为转化指标，小麦成熟胚在预培养基CM4C1P上预培养14d后，用侵染培养液Ⅰ侵染3h，再经干燥共培养方式处理3d，是根癌农杆菌介导成熟胚转化的合适条件。

GCJ8 activate medium, preculture 2 days, 5 X Ca concentration in preculture medium and coculture medium, inoculation I mineture,4mg li1 AgN0 and 0.7% P\P, delay selection 5 days will obtain the highest transformation frequenc Using 'pingyitiancha'shoot apex, mediated by A.tum as above system, the HVAIgene, was transformed into pingyitiancha and obtained Kam resistance rerzeneration plants, it is confirmed that 6 plants of them is transformation plants by identified by PCR and dot blot

按照上述体系，以平邑甜茶茎尖分生组织为受体，将HVA1基因导入平邑甜茶中，获得了具有Kam抗性的再生植株，经PCR与点杂交鉴定，证实有6株为转化植株。

More provocatively, to write is to translate memory into art, an effort to re-member pieces of the past in a mediated form.

更刺激的是，写作就是把回忆翻译成艺术，用间接的形式记录过去片段的努力。

Objective To investigate adenoviral vector mediated exogenous gene expression in mouse lun gs and the effect of mIFN-γ transgene expression on allergen-induced pulmonar y eosinophil infiltration in a murine asthmatic model.

目的研究重组复制缺陷腺病毒在小鼠肺内气道上皮细胞中的转基因表达水平及其介导小鼠γ-干扰素的转基因表达对过敏原诱导的小鼠哮喘模型的预防性治疗作用。

Abstract］ Objective: To provide a suitable fungal material for protoplast-mediated genetic transformation and mutation induction of Pythium guiyangense Su.

目的：建立贵阳腐霉原生质体的制备和再生系统，为该菌的基因工程和细胞工程改良提供适宜的真菌状态。

Object In this study we framed the refusion gene antibacterial peptide[ Cecropin A(l ~ 8)- Magainin (1 - 12),CA(1 ~ 8)-MA(l ~12),CAM] vetor and expressed it in the Escherichia.coli. Thenpurified the recombinded peptide in the Intein Mediated Purificationwith an Affinity Chitin-binding Tag-IMPACT system.

目的 构建重组基因抗菌肽[Cecropin A(1～8)-Magainin(1～12)，CA(1～8)-MA(1～12)，CAM]质粒载体并在大肠杆菌(Escherichia.coli)中表达，应用几丁质自切割分离纯化系统(InteinMediated Purification with an Affinity Chitin-binding Tag，IMPACT)分离纯化抗菌肽，并测定其抗菌活性。

This DNA synthesis was aphidicolin-sensitive, indicating it was mediated by replicative DNA polymerase.

这个DNA合成是aphidicolin敏感的，证明它是通过有复制能力的DNA聚合酶介导的。

We show using in vitro and in vivo models that active TGF-beta1 released during bone resorption coordinates bone formation by inducing migration of bone marrow stromal cells, also known as bone mesenchymal stem cells, to the bone resorptive sites and that this process is mediated through a SMAD signaling pathway.

我们利用体外和体内模型，发现在骨吸收过程中释放的有活性的TGF-β1通过诱导骨髓基质细胞的迁移至骨重吸收位置来调节骨形成，这一过程通过SMAD信号途径调节。

Retinoic acid and its receptors play a very important role in the development of the embryo.RA signal transduction is mainly mediated through it\'s two kinds of receptors, retinoic acid receptor and retinoid X receptor, each kind of receptor has 3 isoforms, a, 6,?.

维甲酸(Retinonic acids，RAs)和其受体在胚胎的发育过程中起着很重要的作用，RA信号的传导主要通过它的两种受体即维甲酸受体和维甲酸类X受体，每种受体又有3种亚型，α，β，γ。

Retinoic acid and its receptors play a very important role in the development of the embryo.RA signal transduction is mainly mediated through its two kinds of receptors, retinoic acid receptor and retinoid X receptor, each kind of receptor has 3 isoforms, a, 6,?.

维甲酸(Retinonic acids，RAs)和其受体在胚胎的发育过程中起着很重要的作用，RA信号的传导主要通过它的两种受体即维甲酸受体和维甲酸类X受体，每种受体又有3种亚型，α，β，γ。

Panorama Many people are dancing in a large sitting room.

在一个大客厅里，许多人在跳舞。

We fully understand how a microwave oven cooks food, it is first necessary to talk ...

我们充分理解一个微波炉煮的食物，这是首先要谈。。。