查询词典 lacking

He must teach himself that the basest of all things is to be afraid,and teach himself that,forget it forever,leaving no room in his workshop for anything but the old verities and truths of the heart,the universal truths lacking which any story is ephemeral and doomed---love and honor and pity and pride and compassion and sacrifice…"

要教会自己在工作中忘记这一切，只在心灵的深处留下恒久的真理与真相，如果这些恒久的、普遍的真理缺失了，那么任何故事都会在瞬间消失----这些真理就是爱、荣誉、怜悯、自豪、同情和牺牲。。。

He must teach himself that the basest of all things is to be afraid: and, teaching himself that, forget it forever, leaving no room in his workshop for anything but the old verities and truths of the heart, the universal truths lacking which any story is ephemeral and doomed love and honor and pity and pride and compassion and sacrifice.

他们必须使自己懂得：一切事物中最基本的东西就是恐惧。而且，要教会自己永远地将恐惧忘却，不要在自己的工作室给它留下任何空间，只留下内心古老的真诚，留下普遍存在的真理——缺少了这种真理，任何故事都是短命的，必遭厄运的——这种普遍的真理即爱情、荣誉、怜悯、自豪、激情和牺牲。

He must teach himself that the basest of all things is to be afraid; and, teaching himself that, forget it forever, leaving no room in his workshop for anything but the old verities and truths of the heart, the universal truths lacking which any story is ephemeral and doomed -- love and honor and pity and pride and compassion and sacrifice.

他必须使自己明白世间最可鄙的事情莫过于恐惧。他必须使自己永远忘却恐惧，在他的工作室里除了心底古老的真理之外，任何东西都没有容身之地。没有这古老的普遍真理，任何小说都只能昙花一观，不会成功；这些真理就是爱、荣誉、怜悯、自尊、同情与牺牲等感情。

When we first met the children who were sitting quietly on the bedsides, we were shocked by their inactivity and lacking of the liveliness of a typical youngster.

见到孩子们的第一印像是每个孩子都安静的坐在床边，没有同年龄孩子应有的活泼。

The positioning of the launcher also precludes the use of a pintle-mounted weapon, and this means, lacking the heavy bolters as well, that the Helios suffers from having no antiinfanry or point defence weaponry.

发射器也挤占了顶置武器站的位置，这意味着缺乏重型爆矢枪的支援，即赫利厄斯的乘员必须要忍受没有反步兵和点防御武器的缺陷。

Herbs without tuberous roots; leaf axils usually lacking woody bulbils; female perianth with the dorsal lobe largest.

没有块茎状的根的草本；叶腋通常缺乏木质的珠芽；雌花花被有大的背侧的裂片 2 L 。

In a post-modern society lacking faith, thousands of people have become Christians after hearing Bach's Cantatas, especially St Matthew Passion.

那里，在一个缺少信仰的后现代(Post-Modern)社会中，有上千群众在听过巴哈的大合唱，尤其是马太福音基督受难记之后成为基督徒。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

In view of lacking effective estimate method and estimate method during the Implement of software company , this paper use the experien ce of CECA National Informatization Evaluation Center for referen ce , establish the model of the Planning Manage System Implement , propose the evaluation index of the Planning Manage System Implement .

针对软件公司在实施过程中缺乏系统有效的评估方法和手段的状况，本文借鉴了国家信息化测评中心的企业信息化指标方案，从软件公司的角度出发，建立了计划管理实施项目绩效评估模型，提出了计划管理绩效评估的指标。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。